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Alexandrium tamarense CCMP1771

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Principle Investigator(s) Mary Ann Moran
External sample ID1771 bacterized initial
NCGR Sample IDMMETSP0380
Sample accession numberCAM_SMPL_002543
Assembly accession numberCAM_ASM_000361
Combined Assembly NameAlexandrium-temarense-CCMP1771
GenusAlexandrium
Speciestamarense
StrainCCMP1771
ClonalYes
AxenicYes
Prelim. NCBI Taxon ID2926
18S rRNA
Importance of organism and transcriptomesAlexandrium tamarense is a model organism for dinoflagellate systems biology because of its relatively small genome (among dinoflagellates), toxin production, and high rates of production of dimethylsulfoniopropionate (DMSP), the precursor to the climate-active gas DMS, which has been implicated in the formation of climate cooling aerosols and clouds. Transcriptomes from axenic versus bacterized A. tamarense blooms will be used to determine the impact of bacterial communities on phytoplankter physiology as well as the production, cycling, and transformation of carbon and DMSP.
Additional citations and referencesMoustafa, A., A. N. Evans, D. M. Kulis, J. D. Hackett, D. L. Erdner, D. M. Anderson, and D. Bhattacharya. 2010. Transcriptome profiling of a toxic dinoflagellate reveals a gene-rich protist and a potential impact on gene expression due to bacterial presence. PLoS ONE 5:e9688. ii. Reference 2 Jasti, S., M. E. Sieracki, N. J. Poulton, M. W. Giewat, and J. N. Rooney-Varga. 2005. Phylogenetic diversity and specificity of bacteria closely associated with Alexandrium spp. and other phytoplankton. Appl. Environ. Microbiol. 71:3483-3494. iii. Reference 3 Howard, E. C., S. Sun, C. R. Reisch, D. A. del Valle, H. Burgmann, R. P. Kiene, and M. A. Moran. 2011. Changes in DMSP demethylase gene assemblages in response to an induced phytoplankton bloom. Appl. Environ. Microbiol. In press.
Environmental Data
Primary citation for organism's characterization, if availableBalech, E. (1995). The genus Alexandrium Halim (Dinoflagellata). pp. 1-151. Sherkin Island: Sherkin Island Marine Station.
Latitude50.6
Longitude4.25
Temperature (ºC)16
pH8.1
Collection date01-JAN-57
Sample collection siteEnglish Channel
Other collection site infoTamar Estuary
Sample material (e.g. "seawater," "sediment," etc.)seawater
ENVO term for habitat - primary termAcquatic: marine
ENVO term for habitat - secondary termNot Applicable
Habitat descriptionTamar Estuary, Plymouth, England, United Kingdom
Habitatmarine habitat
CountryUNITED KINGDOM
Experimental Data
Date of experiment17-MAY-11
Growth mediumF/20 amended seawater
Modifications to growth mediumminus Si
Temperature (ºC)18
Salinty (psu)30
pH6.8
Light (µmol photons / m2 / sec)150
Day portion of day:night cycle in hours16
Night portion of day:night cycle in hours8
Nitrate (μmol/L)88.2
Phosphate (μmol/L)3.62
Trace elements (total) (nmol/L)2450
Investigation typeEukaryotes
Other experimental metadata availablechlorophyll, bacterial production, TOC, inorganic nutrients, particulate and dissolved DMSP, DMS