Marine Microbial Eukaryote Transcriptome Sequencing Project
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Alexandrium tamarense CCMP1771
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| Principle Investigator(s) | Mary Ann Moran |
|---|---|
| External sample ID | 1771 axenic early bloom |
| NCGR Sample ID | MMETSP0382 |
| Sample accession number | CAM_SMPL_002536 |
| Assembly accession number | CAM_ASM_000354 |
| Combined Assembly Name | Alexandrium-temarense-CCMP1771 |
| Genus | Alexandrium |
| Species | tamarense |
| Strain | CCMP1771 |
| Clonal | Yes |
| Axenic | Yes |
| Prelim. NCBI Taxon ID | 2926 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Alexandrium tamarense is a model organism for dinoflagellate systems biology because of its relatively small genome (among dinoflagellates), toxin production, and high rates of production of dimethylsulfoniopropionate (DMSP), the precursor to the climate-active gas DMS, which has been implicated in the formation of climate cooling aerosols and clouds. Transcriptomes from axenic versus bacterized A. tamarense blooms will be used to determine the impact of bacterial communities on phytoplankter physiology as well as the production, cycling, and transformation of carbon and DMSP. |
| Additional citations and references | Moustafa, A., A. N. Evans, D. M. Kulis, J. D. Hackett, D. L. Erdner, D. M. Anderson, and D. Bhattacharya. 2010. Transcriptome profiling of a toxic dinoflagellate reveals a gene-rich protist and a potential impact on gene expression due to bacterial presence. PLoS ONE 5:e9688. ii. Reference 2 Jasti, S., M. E. Sieracki, N. J. Poulton, M. W. Giewat, and J. N. Rooney-Varga. 2005. Phylogenetic diversity and specificity of bacteria closely associated with Alexandrium spp. and other phytoplankton. Appl. Environ. Microbiol. 71:3483-3494. iii. Reference 3 Howard, E. C., S. Sun, C. R. Reisch, D. A. del Valle, H. Burgmann, R. P. Kiene, and M. A. Moran. 2011. Changes in DMSP demethylase gene assemblages in response to an induced phytoplankton bloom. Appl. Environ. Microbiol. In press. |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Balech, E. (1995). The genus Alexandrium Halim (Dinoflagellata). pp. 1-151. Sherkin Island: Sherkin Island Marine Station. |
| Latitude | 50.6 |
| Longitude | 4.25 |
| Temperature (ºC) | 16 |
| pH | 8.1 |
| Collection date | 01-JAN-57 |
| Sample collection site | English Channel |
| Other collection site info | Tamar Estuary |
| Sample material (e.g. "seawater," "sediment," etc.) | seawater |
| ENVO term for habitat - primary term | Acquatic: marine |
| ENVO term for habitat - secondary term | Not Applicable |
| Habitat description | Tamar Estuary, Plymouth, England, United Kingdom |
| Habitat | marine habitat |
| Country | UNITED KINGDOM |
| Experimental Data | |
| Date of experiment | 17-MAY-11 |
| Growth medium | F/20 amended seawater |
| Modifications to growth medium | minus Si |
| Temperature (ºC) | 18 |
| Salinty (psu) | 30 |
| pH | 6.8 |
| Light (µmol photons / m2 / sec) | 150 |
| Day portion of day:night cycle in hours | 16 |
| Night portion of day:night cycle in hours | 8 |
| Nitrate (μmol/L) | 88.2 |
| Phosphate (μmol/L) | 3.62 |
| Trace elements (total) (nmol/L) | 2450 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | chlorophyll, bacterial production, TOC, inorganic nutrients, particulate and dissolved DMSP, DMS |