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Trichosphaerium sp Am-I-7 wt


Principle Investigator(s) Laura Katz
External sample ID40318
Sample accession numberCAM_SMPL_002555
Assembly accession numberCAM_ASM_000373
Combined Assembly NameN/A
StrainAm-I-7 wt
Prelim. NCBI Taxon ID498012
18S rRNA
Importance of organism and transcriptomesThe taxonomic position of Trichosphaerium sp. has been subject to debate. The limited molecular data to date put this taxon on a long branch within the Amoebozoa, perhaps sister to other amoebae with scaled coverings such as Pessonella sp. (ATCC 50979) and Vexillifera minutissima (Lahr et al., submitted). Trichosphaerium sp. has a remarkable life cycle with an alternation between haploid and diploid multinucleate phases (Schaudinn 1899; Angell 1976). Little is known about the ecology of this bizarre organism except that it can feed on seaweeds and other marine algae (Polne-Fuller et al. 1990) and that it can be extremely abundant in some marine systems (Angell 1976).
Additional citations and referencesAngell, R. W. 1976. Observations on Trichosphaerium platyxyrum sp n. J. Protozool. 23:357-364; Schaudinn, F. 1899. Untersuchungen uber den Generationswechsel von Trichosphaerium sieboldi Schn. . Abh. Konigl. Presuss. Suppl.:1-93.; Lahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. Submitted. Comprehensive phylogenetic reconstruction of Amoebozoa based on concatenated analyses of SSU-rDNA and actin genes.
Environmental Data
Primary citation for organism's characterization, if availablePolne-Fuller, M., A. Rogerson, H. Amano, and A. Gibor. 1990. Digestion of Seaweeds by the Marine Ameba Trichosphaerium. Hydrobiologia 204:409-413.
Sample collection siteAtlantic_Ocean
Other collection site infostipes of Sargassum muticum
Sample material (e.g. "seawater," "sediment," etc.)from stipes of Sargassum muticum
ENVO term for habitat - primary termOrganism-Associated
ENVO term for habitat - secondary termOther
Habitat descriptionstipes of Sargassum muticum
Habitatorganism-associated habitat
Experimental Data
Date of experiment11-MAY-11
Growth mediumATCC medium 1405 (HESNW medium)
Temperature (ºC)20
Day portion of day:night cycle in hours16
Night portion of day:night cycle in hours8
Nitrate (μmol/L)549
Ammonium (μmol/L)6
Phosphate (μmol/L)31
Silicate (μmol/L)106
Trace elements (total) (nmol/L)81
Prey organism, if applicable (genus and species)Chlamydomonas reinhardtii (ATCC PRA-142; heat killed)
Investigation typeEukaryotes