Trichosphaerium sp Am-I-7 wt

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Environmental Data
Principle Investigator(s)	Laura Katz
External sample ID	40318
NCGR Sample ID	MMETSP0405
Sample accession number	CAM_SMPL_002555
Assembly accession number	CAM_ASM_000373
Combined Assembly Name	N/A
Genus	Trichosphaerium
Species	sp
Strain	Am-I-7 wt
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	498012
18S rRNA	AGTGATAAGCTTTCTCGAAGGATTAAGCCATGCAAGCTCAGCAAAGAGGTATCCTCGGAGCCGCAAACGGCTCCTTAAAACGGTCGTAACCCACCCGACAAGACTAATAATGGTCACGTCATTTTGCACACAGGAACTTGTTTTCCTAAACGCACTATAACCAAAACTCGTGGCCTCCCCCGCGGATAACCGGGATTAAAGGAAACCTGCGGCTAATACGTAATGCGCCTAGCGCTGCCGATGGGCCCCTCTGCTGCAAGGACCCATCGGTCGCATCCAACGCTCAGGAGCGGACGATCACCCGATCCCATCTTGGTTCGCCAAGGGAAGCAGCCTCCCCTTAGGAAGCGGCGTCCGTACTCCGTCGGGAAGAGCGGCGTTGGGGCATGCGATGGAGTTACGAGGAGTTCTCTCTGACTAGACATGTTTCTAAAAAATAATGTAAAGTTACTTAAGGTGAAACTAGGTGAAAGAAAAATGAACAACTTTGTTCAATCTCGATCCTTCGCCCAAATGTGCTTTTTCATAAAAAAAGATCATATCGATATAAAGTTGAGAGTTCTCCCCCTCCACGAGTCGGAAGGGCGGCCGACCTATCAGCCAGGCGGTAGGGTAACGGCCTACCGCGGCAGTGACGGGTAACGGGGAACTAGGGTTCGGTTCCCGGAGAAGGGGCATTAGATACGGCCCCTGTACTTACGGGTAACAGCAGGCGCGTAACTTGGCCATTGATTGCAAATCGAGCCAGCGACTGAGTGTAACGAGGCCGGCATTTAAACCGGTCGTCGCAATGAGGGGCAACTGAATGTCGCCCTGAGAAACTATTGGAGGACAAGTTGGGTGCCAGCACCCGCGGTAACACCCACTCCGAAAGCTTATACTTTTGCTGTTGCTGTTAAAGCGCTCGCAGTCCAAACAACGACACAGCTGAGAGGGACAAGGTTTATATAGGAGTTTACTCCTTGACAAAACCCCCCGACTCTTTTTCATCGCCCGACCAGGCATAAATGGCTTCGCTCAAACGCAAGGTCAGTTTAGGCTTCGGCCGCTATGCCACCCACCAGCATCCTGGAATTGGGCTTTAAAAAAACAAAAAAACAACACTTTACCAAGTGCGACGCTCAAGTCTCATTTTTAACCAAGTATAAACAATATCCTGATATGCAAGTCTGAGTGAAAACTTATGTTCTTCATTAAGGGAATAAAAACATTGTGAATTACAAAAGTACTTTGAGAAAAAACCTTGAGTTAGTCTTCACACTAATAAAGAACACACGATTACTAGGTGCGTACGGGGTAGAGAGTGTGGTATCAGCGAGGAGTGAAATCTTTTGACCTGATCCAAACTACCCGAAGCGAAGGCGCTCTGCAAGGGCGTGGCCGGTGATCTAGAACGATGGTGGAGGGAGCGAAGCGGATTAGAGACCCGTGTAGTCTCCACAGTAAACGATGCCGACTGAATGGGCGCCGGGCAAACCAAGGCTCGGGGCCCGTTCCCACGGGAAACCGAAGTAATCGGGCCCAGGGGGGAAGTATGGTCGCAAGGCTGAAACTTAAAGGCATTGACGGAGCAACACCACCAGGCTCTGGAGGCTGCGGCTCAATTTGACTCAACGCGCGACAACTTACCGGGATCGGACGCTCTAAGGGGACGAACAGACTGAAGGGTCTTTTCCGAGTTAGCTAACGGTGGTGCATGGTCGTTTTAGGCTCGTGGACTTATCTGTCAGGTTTATTCCGATAACGGGCGAGATCGCTACTGTCACATTGATTCCCCCGCCTCGTGCGGGACCCCTGTGCAGGACTGACGGCGAAAAGTCGTAAGAAGCGGCGGCTATAACAGATCCGGGATGCCCCCAGATATTCCGGGCCGCACGCGGCCTACACTGCTTAGTTCAACGGGTTCCTGGGCCGAACAGGCATCCGGGCAAGCCCTGAAACCGCCAGAACACTTCTTCACGACAGCGAGTTAAACCTCAGATTTTATTATCTAAATACGACTACACTTAAACGCACCTTCGGAAACTACTACCTTCAAAACAAAGTCTGTCCTGAACCTAATATAAAGAGTTAACCCCCTCGTCGAGGCTTTTTAGGAAGTTAAATTCCTTTAATGTTAAGATGAGAAGCGCTGTTAGATGAAAACTTACATCTTTGGACTTGTTCCTTAGTGTAAACATTGAAAGAGCTGTGGATCGTTTCAAATTAACAAACAAGGTTTCTTCTTCACTTAAATGCTTTGACTTGGAAGATTATATCTTTACCAAGGCAAACTTTATTATGAAGCAAGTTTCTGCAATGTTCATAAAAAAATACATGAAAGCGCACCGTGTGGTCGATTTGGATGTTAAGTGTGCTCAATGCTGCTTCTTCGAAAAGACACATGCGTGCTTAGGGACTGGTTGCTGTAACTGTAGCCACGAACGCGGAATGCCTCGTACAGGTGTGTCATCAACGCACCTGGAATCTGTCCCTGTTGCTTGTACACACCGCCCGTCGCATCGACCGATGTGGGTGCGAGTGAGACCAGGGGGGACGACCGGCTTGCCGG
Importance of organism and transcriptomes	The taxonomic position of Trichosphaerium sp. has been subject to debate. The limited molecular data to date put this taxon on a long branch within the Amoebozoa, perhaps sister to other amoebae with scaled coverings such as Pessonella sp. (ATCC 50979) and Vexillifera minutissima (Lahr et al., submitted). Trichosphaerium sp. has a remarkable life cycle with an alternation between haploid and diploid multinucleate phases (Schaudinn 1899; Angell 1976). Little is known about the ecology of this bizarre organism except that it can feed on seaweeds and other marine algae (Polne-Fuller et al. 1990) and that it can be extremely abundant in some marine systems (Angell 1976).
Additional citations and references	Angell, R. W. 1976. Observations on Trichosphaerium platyxyrum sp n. J. Protozool. 23:357-364; Schaudinn, F. 1899. Untersuchungen uber den Generationswechsel von Trichosphaerium sieboldi Schn. . Abh. Konigl. Presuss. Suppl.:1-93.; Lahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. Submitted. Comprehensive phylogenetic reconstruction of Amoebozoa based on concatenated analyses of SSU-rDNA and actin genes.
Primary citation for organism's characterization, if available	Polne-Fuller, M., A. Rogerson, H. Amano, and A. Gibor. 1990. Digestion of Seaweeds by the Marine Ameba Trichosphaerium. Hydrobiologia 204:409-413.
Latitude	34.46809
Longitude	-120.27233
Sample collection site	Atlantic_Ocean
Other collection site info	stipes of Sargassum muticum
Sample material (e.g. "seawater," "sediment," etc.)	from stipes of Sargassum muticum
ENVO term for habitat - primary term	Organism-Associated
ENVO term for habitat - secondary term	Other
Habitat description	stipes of Sargassum muticum
Habitat	organism-associated habitat
Country	UNITED STATES
Experimental Data
Date of experiment	11-MAY-11
Growth medium	ATCC medium 1405 (HESNW medium)
Temperature (ºC)	20
pH	8
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Nitrate (μmol/L)	549
Ammonium (μmol/L)	6
Phosphate (μmol/L)	31
Silicate (μmol/L)	106
Trace elements (total) (nmol/L)	81
Prey organism, if applicable (genus and species)	Chlamydomonas reinhardtii (ATCC PRA-142; heat killed)
Investigation type	Eukaryotes