Heterosigma akashiwo CCMP3107

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Environmental Data
Principle Investigator(s)	Susanne Menden-Deuer, Leo Tolstoy
External sample ID	3107 High Salinity - Dark
NCGR Sample ID	MMETSP0411
Sample accession number	CAM_SMPL_002558
Assembly accession number	CAM_ASM_000376
Combined Assembly Name	Heterosigma-akashiwo-CCMP3107
Genus	Heterosigma
Species	akashiwo
Strain	CCMP3107
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	536047
18S rRNA	TAACTTCCCTCGGCAAGTCACAATAAAGTTCCATGCCAAAGTCCGAGATTTTC ACCGAATCATTCAATCGGTAGGTGCGACGGGCGGTGTGTACAAAGGGCAG GGACGTAATCAATGCGAGCTGATGACTCGCGTTTACTAGGAATTCCTCGT TCAAGATTAATAATTGCAATAATCTATCCCTATCACGATGCACGTTCAAA AGATTACCCGTGCCTTCCGGCAAAGGTCGTTACTCGTTGCATGCATCAGT GTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTGTTATT GCCCCCAACTTCCTTCGGTTAGTCACCGAAAGTCCCTCTAAGAAGTAGAA ACGGCAATGCAGAAGCATTACCGACACTATTTAGCAGGCGGGGGTCTCGT TCGTTAACGGAATTAACCAGACAAATCACTCCACCAACTAAGAACGGCCA TGCACCACCACCCATAGAATCAAGAAAGAGCTATCAATCTGTCAATCCTC ACTATGTCTGGACCTGGTAAGTTTCCCCGTGTTGAGTCAAATTAAGCCGC AGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCC TTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCGGAAGGT GCTGACGGAGTCTAGGTAACGACCGCCAATCCCTAGTCGGCATAGTTTAT GGTTAAGACTACGATGGTATCTAATCATCTTCGATCCCCTAACTTTCGTT CTTGATTAATGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATCCAAGAATTTCACCTCTGACAATTGAATACGAATACCCCCAACTATCCCTATTAATCATTACCTTGGCGTGCAAACCAACAAAATAGAAAACCACCAAGGCCCTATCTCATTATTCCATGCTAATGTATTCAACGGCCTAAGCNTGCTTTGAACACTCTAATTTTTTCACAGTAAAAGATCAGGAGTTCGCAACCCGTAAGTTAATACAAGAAGCGCCTTCCTGAAGGATGGCAAAGGACGATACAGGTGCAGACCCCTTGCGAGGCCGACCGATCACCTCCACCAGAAATCCAACTACGAGCTTTTTAACTGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGTTCCTCGATAAGGGATTTAAATTGTTCTCATTCCAATTGCCGGACTTTAAAAGCCTAGCATTGTTATTTATTGTCACTACCTCCCTATGTCAGGATTGGGTAATTTACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCCAATTCTCCGTTACCCGTTAAAGCCATGGTAGGCCAATACCCTACCATCCGAAGCTGATAGGGCAGAAACTTGAATGAACCATCGACCGAAGTCGATTCGCACAGTTACTATGATTCACCACAAAAACCGAGAAAACTCGGTATTGGTTTCCATCTAATAAATACATCCCGTCCGCCGAAGCAGTTGGGAGTTGATGCATGTATTAGCTCTAGAATTACTACGGTTATCCAAGTAGTAGGGACTATCAAATAAACTATAACTGATATAATGAGCCATTCGCAGTTTCACAGTATAGAGTCGTTTATACTTAGA
Importance of organism and transcriptomes	Blooms of toxic phytoplankton can negatively impact coastal ecosystems and economies through poisoning events that induce high mortality rates in fish (Landsberg 2002), birds (Shumway et al. 2003; Jessup et al. 2009), and illness in humans (Van Dolah et al. 2001). Heterosigma akashiwo is a common, estuarine HAB species that induces mortality in wild and aquaculture fish. We have been investigating the physiological and behavioral basis for H. akashiwo success and found that an interplay between a broad halotolerance and toxicity to some heterotrophic protists is key to predicting this HABs population dynamics (Fredrickson et al. 2009, Menden-Deuer et al. 2010, Harvey & Menden-Deuer 2011, Strom et al. in prep). This transcriptome will provide us with the opportunity to investigate the genetic basis for the halotolerance and toxicity of this strain, compare it to another strain and investigate toxicity and ecology in-situ to support predictive modeling of HAB occurrences.
Additional citations and references	Smayda et al 1993 Toxic Phytoplankton Blooms in the Sea
Primary citation for organism's characterization, if available	doi:10.4319/lo.2011.56.1.0371
Latitude	52.5758
Longitude	-128.5003
Collection date	15-SEP-08
Sample collection site	Pacific_Ocean
Other collection site info	Nowish Inlet, Queen Charlotte Sound
ENVO term for habitat - primary term	Acquatic: marine
Habitat	marine habitat
Country	CANADA
Experimental Data
Date of experiment	16-AUG-11
Growth medium	f/2-Si amended seawater
Modifications to growth medium	f/2 for all nutrients except eliminate Si
Temperature (ºC)	15
Salinty (psu)	29.4
Light (µmol photons / m2 / sec)	75
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	106
Investigation type	Eukaryotes
Other experimental metadata available	not axenic