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Heterosigma akashiwo CCMP3107

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Principle Investigator(s) Susanne Menden-Deuer, Leo Tolstoy
External sample ID3107 High Salinity - Dark
NCGR Sample IDMMETSP0411
Sample accession numberCAM_SMPL_002558
Assembly accession numberCAM_ASM_000376
Combined Assembly NameHeterosigma-akashiwo-CCMP3107
GenusHeterosigma
Speciesakashiwo
StrainCCMP3107
ClonalYes
AxenicNo
Prelim. NCBI Taxon ID536047
18S rRNA
Importance of organism and transcriptomesBlooms of toxic phytoplankton can negatively impact coastal ecosystems and economies through poisoning events that induce high mortality rates in fish (Landsberg 2002), birds (Shumway et al. 2003; Jessup et al. 2009), and illness in humans (Van Dolah et al. 2001). Heterosigma akashiwo is a common, estuarine HAB species that induces mortality in wild and aquaculture fish. We have been investigating the physiological and behavioral basis for H. akashiwo success and found that an interplay between a broad halotolerance and toxicity to some heterotrophic protists is key to predicting this HABs population dynamics (Fredrickson et al. 2009, Menden-Deuer et al. 2010, Harvey & Menden-Deuer 2011, Strom et al. in prep). This transcriptome will provide us with the opportunity to investigate the genetic basis for the halotolerance and toxicity of this strain, compare it to another strain and investigate toxicity and ecology in-situ to support predictive modeling of HAB occurrences.
Additional citations and referencesSmayda et al 1993 Toxic Phytoplankton Blooms in the Sea
Environmental Data
Primary citation for organism's characterization, if availabledoi:10.4319/lo.2011.56.1.0371
Latitude52.5758
Longitude-128.5003
Collection date15-SEP-08
Sample collection sitePacific_Ocean
Other collection site infoNowish Inlet, Queen Charlotte Sound
ENVO term for habitat - primary termAcquatic: marine
Habitatmarine habitat
CountryCANADA
Experimental Data
Date of experiment16-AUG-11
Growth mediumf/2-Si amended seawater
Modifications to growth mediumf/2 for all nutrients except eliminate Si
Temperature (ºC)15
Salinty (psu)29.4
Light (µmol photons / m2 / sec)75
Day portion of day:night cycle in hours12
Night portion of day:night cycle in hours12
Nitrate (μmol/L)882
Phosphate (μmol/L)36.2
Silicate (μmol/L)106
Investigation typeEukaryotes
Other experimental metadata availablenot axenic