Filamoeba nolandi NC-AS-23-1

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Environmental Data
Principle Investigator(s)	Laura Katz
External sample ID	50430
NCGR Sample ID	MMETSP0413
Sample accession number	CAM_SMPL_002547
Assembly accession number	CAM_ASM_000365
Combined Assembly Name	N/A
Genus	Filamoeba
Species	nolandi
Strain	NC-AS-23-1
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	136452
18S rRNA	atcctgccagtagtcatatgcttgtctcaaagattaagccatgcatgtctaagaataaccccagtaaggggtagtctgcgaatggctcattaaatcagttatagtttatttggatgtgggcttgctacacggataactgtggcaattctagagctaatacgtgctaagtccccaacttcgcggaaggggtgtatgtgttaggtactcaactggctcgctggttgattcataacagccttaactgaccgcatagccttgcgctggcggcgtcgcattcaaatttctgatctatcaacttgaaggtagggtagaggcctaccttggttgtaacggataacggggaattagggttcggttccggagagggcgcctgagagatggcgaccacttccaaggaaggcagcaggcgcgcaaattacccaatgctaatacagcgaggtagtgacaataaataacaatgtcggggtcttaaagcctcggcaattggaatgagcacaatttaaatcccttrgcgaggaacaactagaggacaagtccggtgccagcacccgcggtaattccggctctagtagcgtatattaaagttgttgcagttaaaaagctcgtagtctgacttcgggctggactgtgaaggctcaccgcaaggtgggacggatagtctggccttatcctctgatgtgctctctgctcttaactgagcggatctgtcgcacaggggcgtttaccttgagcaaattgaggtgttcaaggcaggcatatatgctcgaacaatgtagcatgggataatggaatacggcctcggacttgcttttgctggttggtgagtccgggcaatgatcaacaggggcggtcggggtgcttcatatgcaggcgctagaggtgaaattcttggaccgtctgcagatgcccagatgcgaaagcgttgcaccagtacgtccccattgatcaagaacgaaagttaagggagcgaagacgatcagataccgtcgtagtcttaaccctaaacgataccgaccagggatcgaggacagtcccatggttgactccttcggaaccttgtgggaaaccacgagtctttgggttctggggggaatatggtcgcaaggctgaaacttaaaggaattgacggaagggcacaccaggagtggarcctgcggcttaatttgactcaacacggggaaactcactcggtcaggacatagtaaggattgacaggttgacggtcctttcttgattctatgggtggtggtgcatggtcgttcttagttcgtggagtgatctgtctggttaattccgctaacggacgagacctctccttattaactagtgcggcccgcggccgtaagggtgaagcggtagagctggttggtgcttcggtgctgactgcttagccggggaacctggcgtgcgggctgtttacttcttaggggtacaatctgcgttttagcagaggggagctagaggcaataacaggtctgtgatgcccttagatgttcgaggctgcacgcgcgctacaatggtatggtcagcgagttcctggctcgaaagggttggggaatcacatgaatccatatcgtgctggggatagatgattgcaattattcgtcttcaacgaggaattcctagtaagtgcacttcatcagagtgcgctgattgcgtccctgccctttgtacacaccgcccgtcgctcctaccgattgaacggtccggtgaaatctacggatattgggacctaaggggcaaccctaagtctcgatagaagttgagtaaaccttactgtttagaggaaggagaagtcgtaacaaggtttccgtaggtgaacctgcagaaggatca
Importance of organism and transcriptomes	The genus Filamoeba has been placed within the Lobosea, closely related to the classic lobose amoebae Amoeba and Chaos (Amoebida Page 1967). Molecular data place Filamoeba nolandi in the newly described group Flammellidae, along with other filopodia producing Amoebozoa (Kudryavtsev et al. 2009; Lahr et al., submitted). Filamoeba spp. can be free living and/or can be parasites of fish (Dykova and Lom 2004). Lineages grouping with Filamoeba nolandi have been found in habitats as diverse as coastal waters and the naturally polluted Rio Tinto, a river with a pH of 2 and high levels of metals attributed to its waters draining from iron-rich deposits (Amaral Zettler et al. 2002). There are only two described species, but given variable ssu-rDNA sequences and its remarkable environmental range, it is likely that there is considerable undescribed diversity here that will benefit from a transcriptome dataset.
Additional citations and references	Amaral Zettler, L. A. A., F. Gomez, E. Zettler, B. G. Keenan, R. Amils, and M. L. Sogin. 2002. Eukaryotic diversity in Spain's River of Fire - This ancient and hostile ecosystem hosts a surprising variety of microbial organisms. Nature 417:137-137.; Lahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. Submitted. Comprehensive phylogenetic reconstruction of Amoebozoa based on concatenated analyses of SSU-rDNA and actin genes.;Dykova, I., and J. Lom. 2004. Advances in the knowledge of amphizoic amoebae infecting fish. Folia Parasitol. (Praha). 51:81-97.; Kudryavtsev, A., C. Wylezich, M. Schlegel, J. Walochnik, and R. Michel. 2009. Ultrastructure, SSU rRNA gene sequences and phylogenetic relationships of Flamella Schaeffer, 1926 (Amoebozoa), with description of three new species. Protist 160:21-40.
Primary citation for organism's characterization, if available	Amaral Zettler, L. A., T. A. Nerad, C. J. O'Kelly, M. T. Peglar, P. M. Gillevet, J. D. Silberman, and M. L. Sogin. 2000. A molecular reassessment of the leptomyxid amoebae. Protist 151:275-282.
Latitude	34.22573
Longitude	-77.94471
Sample collection site	Atlantic_Ocean
Sample material (e.g. "seawater," "sediment," etc.)	sediment core 23 nautical miles off coast of Wilmington, NC
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Sediment
Habitat description	sediment core 23 nautical miles off coast of Wilmington, NC
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	14-MAY-11
Growth medium	Sonneborn's Paramecium medium (ATCC medium 802)
Temperature (ºC)	20
pH	7
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Prey organism, if applicable (genus and species)	Enterobacter aerogenes ATCC 13048 and mixed bacteria
Investigation type	Eukaryotes
Other experimental metadata available	Entries above were left blank as medium 802 is an undefined medium