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Heterosigma akashiwo NB


Principle Investigator(s) Susanne Menden-Deuer
External sample IDHA NB High Salinity - Light
Sample accession numberCAM_SMPL_002550
Assembly accession numberCAM_ASM_000368
Combined Assembly NameHeterosigma-akashiwo-NB
Prelim. NCBI Taxon ID536047
18S rRNA
Importance of organism and transcriptomesBlooms of toxic phytoplankton can negatively impact coastal ecosystems and economies through poisoning events that induce high mortality rates in fish (Landsberg 2002), birds (Shumway et al. 2003; Jessup et al. 2009), and illness in humans (Van Dolah et al. 2001). Heterosigma akashiwo is a common, estuarine HAB species that induces mortality in wild and aquaculture fish. We have been investigating the physiological and behavioral basis for H. akashiwo success and found that an interplay between a broad halotolerance and toxicity to some heterotrophic protists is key to predicting this HABs population dynamics (Fredrickson et al. 2009, Menden-Deuer et al. 2010, Harvey & Menden-Deuer 2011, Strom et al. in prep). This transcriptome will provide us with the opportunity to investigate the genetic basis for the halotolerance and toxicity of this strain, compare it to another strain and investigate toxicity and ecology in-situ to support predictive modeling of HAB occurrences.
Additional citations and referencesFredrickson, K.A., S.L. Strom, R. Crim and K. Coyne. Inter-strain variability in physiology and genetics of Heterosigma akashiwo (Rahpidophyceae) from the west coast of North America. In press, Journal Phycology.Harvey E.L. and S. Menden-Deuer. 2011. Avoidance, movement, and mortality: The interactions between a protistan grazer and Heterosigma akashiwo, a harmful algal bloom species. L&O. 56(1): 371-378
Environmental Data
Primary citation for organism's characterization, if availableSmayda et al 1993 Toxic Phytoplankton Blooms in the Sea
Salinity (psu)25.6
Temperature (ºC)21.2
Collection date18-JUN-10
Collection time07:05 AM
Sample collection siteAtlantic_Ocean
Other collection site infoGreenwich Cove, Narragansett Bay
Sample material (e.g. "seawater," "sediment," etc.)Seawater
ENVO term for habitat - primary termAcquatic: marine
Habitat descriptionEstuary
Habitatmarine habitat
Experimental Data
Date of experiment07-SEP-11
Growth mediumf/2-Si amended seawater
Modifications to growth mediumf/2 for all nutrients except eliminate Si
Temperature (ºC)15
Salinty (psu)29.4
Light (µmol photons / m2 / sec)75
Day portion of day:night cycle in hours12
Night portion of day:night cycle in hours12
Nitrate (μmol/L)882
Phosphate (μmol/L)36.2
Silicate (μmol/L)106
Investigation typeEukaryotes
Other experimental metadata availablenot axenic