Marine Microbial Eukaryote Transcriptome Sequencing Project
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Heterosigma akashiwo NB
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| Principle Investigator(s) | Susanne Menden-Deuer |
|---|---|
| External sample ID | HA NB Low Salinity |
| NCGR Sample ID | MMETSP0415 |
| Sample accession number | CAM_SMPL_002551 |
| Assembly accession number | CAM_ASM_000369 |
| Combined Assembly Name | Heterosigma-akashiwo-NB |
| Genus | Heterosigma |
| Species | akashiwo |
| Strain | NB |
| Clonal | Yes |
| Axenic | No |
| Prelim. NCBI Taxon ID | 536047 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Blooms of toxic phytoplankton can negatively impact coastal ecosystems and economies through poisoning events that induce high mortality rates in fish (Landsberg 2002), birds (Shumway et al. 2003; Jessup et al. 2009), and illness in humans (Van Dolah et al. 2001). Heterosigma akashiwo is a common, estuarine HAB species that induces mortality in wild and aquaculture fish. We have been investigating the physiological and behavioral basis for H. akashiwo success and found that an interplay between a broad halotolerance and toxicity to some heterotrophic protists is key to predicting this HABs population dynamics (Fredrickson et al. 2009, Menden-Deuer et al. 2010, Harvey & Menden-Deuer 2011, Strom et al. in prep). This transcriptome will provide us with the opportunity to investigate the genetic basis for the halotolerance and toxicity of this strain, compare it to another strain and investigate toxicity and ecology in-situ to support predictive modeling of HAB occurrences. |
| Additional citations and references | Fredrickson, K.A., S.L. Strom, R. Crim and K. Coyne. Inter-strain variability in physiology and genetics of Heterosigma akashiwo (Rahpidophyceae) from the west coast of North America. In press, Journal Phycology.Harvey E.L. and S. Menden-Deuer. 2011. Avoidance, movement, and mortality: The interactions between a protistan grazer and Heterosigma akashiwo, a harmful algal bloom species. L&O. 56(1): 371-378 |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Smayda et al 1993 Toxic Phytoplankton Blooms in the Sea |
| Latitude | 41.65583 |
| Longitude | -71.44778 |
| Depth (m) | 1 |
| Salinity (psu) | 25.6 |
| Temperature (ºC) | 21.2 |
| Collection date | 18-JUN-10 |
| Collection time | 07:05 AM |
| Sample collection site | Atlantic_Ocean |
| Other collection site info | Greenwich Cove, Narragansett Bay |
| Sample material (e.g. "seawater," "sediment," etc.) | Seawater |
| ENVO term for habitat - primary term | Acquatic: marine |
| Habitat description | Estuary |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 07-SEP-11 |
| Growth medium | f/2-Si amended seawater |
| Modifications to growth medium | f/2 for all nutrients except eliminate Si |
| Temperature (ºC) | 15 |
| Salinty (psu) | 15 |
| Day portion of day:night cycle in hours | 12 |
| Night portion of day:night cycle in hours | 12 |
| Nitrate (μmol/L) | 882 |
| Phosphate (μmol/L) | 36.2 |
| Silicate (μmol/L) | 106 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | not axenic |