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Pessonella sp. PRA-29


Principle Investigator(s) Laura Katz
External sample IDPRA-29
Sample accession numberCAM_SMPL_002560
Assembly accession numberCAM_ASM_000378
Combined Assembly NameN/A
Prelim. NCBI Taxon ID498008
18S rRNA
Importance of organism and transcriptomesPessonella has been placed in the Dactylopodida (Amoebozoa) based on morphology (Smirnov et al. 2005) and molecular data place this taxon close to the Dactylopodids, but without support (Tekle et al. 2008; Lahr et al, submitted). Pessonella has a distinctive outer membrane covering like many of the Thecamoebida, but members of the Thecamoebida do not seem to be closely related to Pessonella sp. in molecular reconstructions (Smirnov et al. 2005; Tekle et al. 2008; Lahr et al, submitted). However, only a few molecular data (ssu-rDNA and actin) exist for this lineage. The isolate at ATCC was taken from a lobster, though it can also be grown as a bacterivore in standard media.
Additional citations and referencesTekle, Y. I., J. R. Grant, O. R. Anderson, J. C. Cole, T. A. Nerad, D. J. Patterson, and L. A. Katz. 2008. Phylogenetic placement of diverse amoebae inferred from multigene analyses and assessment of clade stability within `Amoebozoa? upon removal of varying rate classes of SSU-rDNA. Mol. Phylogenet. Evol. 47:339-352.; Smirnov, A., E. Nassonova, C. Berney, J. Fahrni, I. Bolivar, and J. Pawlowski. 2005. Molecular phylogeny and classification of the lobose amoebae. Protist 156:129-142.; Lahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. Submitted. Comprehensive phylogenetic reconstruction of Amoebozoa based on concatenated analyses of SSU-rDNA and actin genes.
Environmental Data
Sample collection siteAtlantic_Ocean
Other collection site infoisolated from a lobster
Sample material (e.g. "seawater," "sediment," etc.)marine invertebrate
ENVO term for habitat - primary termOrganism-Associated
ENVO term for habitat - secondary termOther
Habitat descriptionisolated from a lobster
Habitatorganism-associated habitat
Experimental Data
Date of experiment29-APR-11
Growth mediumATCC medium 1525 (Seawater 802 medium)
Temperature (ÂșC)20
Day portion of day:night cycle in hours16
Night portion of day:night cycle in hours8
Prey organism, if applicable (genus and species)Klebsiella pneumoniae subsp. pneumoniae ATCC 700831
Investigation typeEukaryotes
Other experimental metadata availableEntries above were left blank as medium 1525 is an undefined medium