Pessonella sp. PRA-29

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Environmental Data
Principle Investigator(s)	Laura Katz
External sample ID	PRA-29
NCGR Sample ID	MMETSP0420
Sample accession number	CAM_SMPL_002560
Assembly accession number	CAM_ASM_000378
Combined Assembly Name	N/A
Genus	Pessonella
Species	sp.
Strain	PRA-29
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	498008
18S rRNA	taatcatacgcttgtctcaaagattaaaccatgcaagtctaagtttaacgaaatttaaacacgaagacagcgaatatctccttaaatcagcaatcgtttacttgattatttctactttttgaattattaaaagttgtttctcggataactgtggtaattctagagctaatacgtattaaaatgggcgctgtgagttttcgaacgatcggcgtcttgtatttattagattttcaaaaaccaatatagatcattccaaactcgcgtttcggcgcggggatctattccttatttctattggtgaatcataataactgatcgaaccgtgtgtcgcaagacgacggtgtttcattcaaatttctgccctatcagctttcgttggtagtgtagtggactaccaaggcttttacgggtacggggaattagagtttgattccggagagggagcctgagaaacggctaccacttccaaggaaggcagcaggtgcgaaaattacccaattccatctcggagaggtagtgacaaaaaataacaatactggacctgataaaggtcttgtaattgcaatgagtacaatttaaaccaattaacaaggatccaatagagggcaagcctggtgccagcagccgcggtaattccagctctgtgagtgtatattagatttgttgtagttaaaaagctcgtagtcggagatgagactatttttttgaaatttgaaagcgggattcgagtggaaaattctgctcgggttcacttttaatttcgatagtcttttaatgagcaatcaagttagcctcacggcgctcgtttgctctgtttactttgaaaaaattagagtgttcaaagcaaggaaaagattgcacctcacggtgcgtctttgatttaaaccttttgaatattttagcatgggataatagaacaagacctttttttgattttattggttttccatttttaattattaggtaatgattaacagggacagttgggggtattagtattgcgttgtcagaggtgaaattcttggattaacgcaggactaacttttgcgaaggcatttaccaaggatgttttcattgatcaagaacgatagtttggggatcgaagatgattagacaccgttgtagtccaaactgtaaactatgccggccctggatgtgcaaacgtcactttaacatagtcgcggttttcgattagcagcaatgcgtcgttaaaactcgactacctagactttgtgcgcacagtaagagaaattacaagcgtttgggttctagggggagtatggtcgcaagactggaacttaaaggaattgacggaaggccaccacaagttgtggaacgtgcggcttaatttgagtcaacacgggaaaactttccagggcaagacggaatgaggattgacagattgagagctctttcttgatttttcggttagtggtgcatggccgctcttagttggtgctttgaagtgtctggttaattccgataacgagcgagacctcaacttactaaatagttgaacaaattttcgaaaacctcgttgatatgaagcggagttttaatgaaatatttaatcgtttttcattcgcgccgtggattattgtttttaaatttgttttctttgtgcgggcgtttcggcgcgcgcatgaaatacttcttagaaggactttttgtattgaaacaaaaggaagtttgaggaaatagcaggtctgtaatssccttagatgttctggaatgcacgcgcgttactattgaaaaagaaaaaagtatataaaaagttgccggacttgtttcggtgcttttgaaacctatctcgagagagaatgccagctttagtgcgcttcggtgtatcggctggcgatgggtaatctttgaaatctttttatcaattgggatagatgattgcaattcttcatcttgaacgaggaatatctagtaaacgcgactcatcaagtcgcgttgactacgtcactggcctttgtacacaccgcccgtcgcttctaccgattgatggttgaggtgaggttttgggacgacctatggaaagtattgcttcctttttgagttgcaaagctgtttgggcractgaacagttcctttcctgtcgaaacttgcccgaatctttatcattagaggaagaagaagtcgtaacaaggtttncctg
Importance of organism and transcriptomes	Pessonella has been placed in the Dactylopodida (Amoebozoa) based on morphology (Smirnov et al. 2005) and molecular data place this taxon close to the Dactylopodids, but without support (Tekle et al. 2008; Lahr et al, submitted). Pessonella has a distinctive outer membrane covering like many of the Thecamoebida, but members of the Thecamoebida do not seem to be closely related to Pessonella sp. in molecular reconstructions (Smirnov et al. 2005; Tekle et al. 2008; Lahr et al, submitted). However, only a few molecular data (ssu-rDNA and actin) exist for this lineage. The isolate at ATCC was taken from a lobster, though it can also be grown as a bacterivore in standard media.
Additional citations and references	Tekle, Y. I., J. R. Grant, O. R. Anderson, J. C. Cole, T. A. Nerad, D. J. Patterson, and L. A. Katz. 2008. Phylogenetic placement of diverse amoebae inferred from multigene analyses and assessment of clade stability within `Amoebozoa? upon removal of varying rate classes of SSU-rDNA. Mol. Phylogenet. Evol. 47:339-352.; Smirnov, A., E. Nassonova, C. Berney, J. Fahrni, I. Bolivar, and J. Pawlowski. 2005. Molecular phylogeny and classification of the lobose amoebae. Protist 156:129-142.; Lahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. Submitted. Comprehensive phylogenetic reconstruction of Amoebozoa based on concatenated analyses of SSU-rDNA and actin genes.
Sample collection site	Atlantic_Ocean
Other collection site info	isolated from a lobster
Sample material (e.g. "seawater," "sediment," etc.)	marine invertebrate
ENVO term for habitat - primary term	Organism-Associated
ENVO term for habitat - secondary term	Other
Habitat description	isolated from a lobster
Habitat	organism-associated habitat
Country	UNITED STATES
Experimental Data
Date of experiment	29-APR-11
Growth medium	ATCC medium 1525 (Seawater 802 medium)
Temperature (ºC)	20
pH	8
Day portion of day:night cycle in hours	16
Night portion of day:night cycle in hours	8
Prey organism, if applicable (genus and species)	Klebsiella pneumoniae subsp. pneumoniae ATCC 700831
Investigation type	Eukaryotes
Other experimental metadata available	Entries above were left blank as medium 1525 is an undefined medium