Home :: List of Publicly Available Datasets :: Sexangularia sp. ATCC50979

Sexangularia sp. ATCC50979


Principle Investigator(s) Laura Katz
External sample ID50979
Sample accession numberCAM_SMPL_002573
Assembly accession numberCAM_ASM_000391
Combined Assembly NameN/A
Prelim. NCBI Taxon ID0
Importance of organism and transcriptomesThe isolate deposited as ATCC 50979 with the identification Sexangularia sp. is in fact not a testate amoeba but has morphology consistent with the Dactylopodida. We are currently describing this taxon as a new genus. Preliminary transmission electron microscopy has shown that this organism is covered in oval shaped scales, providing an interesting link between the Dactylopodida and the Vannelida. An actin analysis including this taxon shows grouping with another enigmatic genus, Trichosphaerium (Lahr, Katz, et al. In prep). We characterized actin and beta-tubulin from this taxon as part of NSF funded ATOL project, though we were unable to amplify ssu-rDNA (Lahr, Katz, et al. In prep). The strain available at ATCC is marine and was isolated from a mud flat and deposited by Tom Nerad, former director of the protistology collections. Molecular data will inform not only studies on the ecology and physiology of this lineage, but will also be essential for elucidating the phylogenetic details of Dactylopodida and Vannelida lineages.
Additional citations and references
Environmental Data
Primary citation for organism's characterization, if availableLahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. Submitted. Comprehensive phylogenetic reconstruction of Amoebozoa based on concatenated analyses of SSU-rDNA and actin genes.
Sample collection siteAtlantic_Ocean
Other collection site infoisolated from a mud flat
Habitatmarine habitat
Experimental Data
Growth mediumATCC medium 1525 (Seawater 802 medium)
Modifications to growth medium0.02% FBS plus antibiotics to prevent bacterial overgrowth.
Temperature (ÂșC)20
Day portion of day:night cycle in hours16
Night portion of day:night cycle in hours8
Prey organism, if applicable (genus and species)Enterobacter aerogenes (ATCC 13048)
Investigation typeEukaryotes
Other experimental metadata availableEntries above were left blank as medium 1525 is an undefined medium.