Marine Microbial Eukaryote Transcriptome Sequencing Project
Home :: List of Publicly Available Datasets :: Sexangularia sp. ATCC50979
Sexangularia sp. ATCC50979
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| Principle Investigator(s) | Laura Katz |
|---|---|
| External sample ID | 50979 |
| NCGR Sample ID | MMETSP0437 |
| Sample accession number | CAM_SMPL_002573 |
| Assembly accession number | CAM_ASM_000391 |
| Combined Assembly Name | N/A |
| Genus | Sexangularia |
| Species | sp. |
| Strain | ATCC50979 |
| Clonal | No |
| Axenic | No |
| Prelim. NCBI Taxon ID | 0 |
| 18S rRNA | N/A |
| Importance of organism and transcriptomes | The isolate deposited as ATCC 50979 with the identification Sexangularia sp. is in fact not a testate amoeba but has morphology consistent with the Dactylopodida. We are currently describing this taxon as a new genus. Preliminary transmission electron microscopy has shown that this organism is covered in oval shaped scales, providing an interesting link between the Dactylopodida and the Vannelida. An actin analysis including this taxon shows grouping with another enigmatic genus, Trichosphaerium (Lahr, Katz, et al. In prep). We characterized actin and beta-tubulin from this taxon as part of NSF funded ATOL project, though we were unable to amplify ssu-rDNA (Lahr, Katz, et al. In prep). The strain available at ATCC is marine and was isolated from a mud flat and deposited by Tom Nerad, former director of the protistology collections. Molecular data will inform not only studies on the ecology and physiology of this lineage, but will also be essential for elucidating the phylogenetic details of Dactylopodida and Vannelida lineages. |
| Additional citations and references | |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Lahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. Submitted. Comprehensive phylogenetic reconstruction of Amoebozoa based on concatenated analyses of SSU-rDNA and actin genes. |
| Latitude | 37.96053 |
| Longitude | -75.3281 |
| Sample collection site | Atlantic_Ocean |
| Other collection site info | isolated from a mud flat |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Growth medium | ATCC medium 1525 (Seawater 802 medium) |
| Modifications to growth medium | 0.02% FBS plus antibiotics to prevent bacterial overgrowth. |
| Temperature (ºC) | 20 |
| pH | 8 |
| Day portion of day:night cycle in hours | 16 |
| Night portion of day:night cycle in hours | 8 |
| Prey organism, if applicable (genus and species) | Enterobacter aerogenes (ATCC 13048) |
| Investigation type | Eukaryotes |
| Other experimental metadata available | Entries above were left blank as medium 1525 is an undefined medium. |