Marine Microbial Eukaryote Transcriptome Sequencing Project
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Stereomyxa ramosa Chinc5
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| Principle Investigator(s) | Laura Katz |
|---|---|
| External sample ID | 50982 |
| NCGR Sample ID | MMETSP0439 |
| Sample accession number | CAM_SMPL_002575 |
| Assembly accession number | CAM_ASM_000393 |
| Combined Assembly Name | N/A |
| Genus | Stereomyxa |
| Species | ramosa |
| Strain | Chinc5 |
| Clonal | No |
| Axenic | No |
| Prelim. NCBI Taxon ID | 0 |
| 18S rRNA | N/A |
| Importance of organism and transcriptomes | Based on morphology, Stereomyxa has been placed in its own group, the Stereomyxidae (Grell 1966), and remains an Incertae sedis taxon (Smirnov et al. 2005). Stereomyxa has a strange combination of pseudopodial types, such as the formation of large branching nets as in the Leptomyxids and also the production of filose pseudopodia. This taxon also has a unique centriole-like body, a radiating microtubule organizing center (MTOC), located in the cytoplasm, that is reminiscent of that of the Acanthamoebidae. Transcriptome data will aid future studies of the physiology of this amoeba, which is able to digest complex seaweed polysaccharides (Armstrong et al. 2000). |
| Additional citations and references | Grell, K. G. 1966. Amoben der famillie Stereomyxidae. Archiv fur Protistenkunde 109:147-154; Smirnov, A., E. Nassonova, C. Berney, J. Fahrni, I. Bolivar, and J. Pawlowski. 2005. Molecular phylogeny and classification of the lobose amoebae. Protist 156:129-142. |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Lahr, D. J. G., J. R. Grant, T. Nguyen, J. H. Lin, and L. A. Katz. In prep. Phylogenetic reconstruction of Amoebozoa based on concatenated analysis of SSU-rDNA and actin genes, with placement of 13 new isolates. |
| Latitude | 37.93318 |
| Longitude | -75.37881 |
| Sample collection site | Atlantic_Ocean |
| Other collection site info | moist soil from mud flat approximately 800 yards from the ocean |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 15-APR-11 |
| Growth medium | ATCC medium 1525 (Seawater 802 medium) |
| Modifications to growth medium | Diluted 1:4 in ATCC medium 1405 (HSNW medium) |
| Temperature (ºC) | 20 |
| pH | 8 |
| Day portion of day:night cycle in hours | 16 |
| Night portion of day:night cycle in hours | 8 |
| Prey organism, if applicable (genus and species) | Klebsiella pneumoniae (ATCC 700831) |
| Investigation type | Eukaryotes |
| Other experimental metadata available | Entries above were left blank as medium 1525 is an undefined medium |