Heterocapsa triquestra CCMP 448

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Environmental Data
Principle Investigator(s)	Susanne Menden-Deuer
External sample ID	Heterocapsa Alone
NCGR Sample ID	MMETSP0448
Sample accession number	CAM_SMPL_002577
Assembly accession number	CAM_ASM_000395
Combined Assembly Name	N/A
Genus	Heterocapsa
Species	triquestra
Strain	CCMP 448
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	66468
18S rRNA	GTTCACTAAA CTTTCCGCTGCAACGTTCGTGACCGAACACTGCTGCAGTCCGAATTATTC ACCGGATCACTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAG GGACGTAATCAGCACGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGT TGAAGATTAATAATTGCAATAATCTATCCCCATCACGATGCGTTTTAAGA AGATTACCCAACCCTTCCGGGCAAGGTCATAAACTCGTTGAGCGCATCAG TGTAGCGCGCGTGCAGCCCAGAACATCTAAGGGCATCACAGACCTGTTAT TGCCTCAAACTTCCTTGCGTTAGACACGCAAAGTCCCTCTAAGAAGTAGC CCACGTAACCGAGGTTACGTGTTACTATTTAGCAGGTTAAGGTCTCGTTC GTTAACGGAATTAACCAGACAAATCACTCCACCAACTAAGAACGGCCATG CACCACCACCCATAGAATCAAGAAAGAGCTATCAATCTGTCAATCCTTAC TATGTCTGGACCTGGTAAGTTTCCCCGTGTTGAGTCAAATTAAGCCGCAG GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTT GCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGC TGAAGGAGTCGTAATGATAACGACCTCCAATCTCTAGTCGGCATGGTTTA TGGTTAAGACTAGGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGT TCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTCCGTCT TTAACAAATCCAAGAATTTCACCTCTGACAGTTAAATACGAATGCCCCCA ACTATCCCTATTAACCATTACCTCAGATCTAGAAACCAACAAAATAGAAC TTGGCAAATGCTTTCGCAGTAGTCCGTCTTTAACAAATCCAAGAATTTCACNTCTGACAGTTAAATACGAATGCCCCCAACTATCCCTATTAACCATTACCTCAGATCTAGAAACCAACAAAATAGAACCGAGGTCCTATCTTATTATTCCATGCTAATGTATTCAAGGCGTGTGCCTGCTTGAAACACTCTAATTTCCTCAAAGTAAAAGTCCTGAATACCGCACCACACAGTCAAGTGCAGTTACGTTCTCCAAGAAGATGCCCAGGCCGAGCCAGATACTCACCCAGAGGGCGGACCGGTCGTCCTCGGCAGAAATCCAACTACGAGCTTTTTAACCGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATCGATACTCGTAAAGGGATTTAAATTCTACTCATTCCAATTACAAGACATGGATGCCCTGTATTGTTATTTCTTGTCACTACCTCCCTGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTTACCCGTCATTGCCACGGTAGGCCAATACCCTACCGTCGGAAGCTGATAGGTCAGAAACTTGAATGATTCATCGCCAGCGGATGCCATGCGATTCGTTCGGTTATTATGAATCACCAGAAGACCAGGCGGAGCCTGGGTTGGTTTTGTAACTAATAAACACAACCCTTCCAAGAAGTCGGGTTTTGGCGCATGTATTAGCTCTAGAATTACCACGGTTATCCATGTAAGAATGACCATCAAATAAACTATAACTGTTTTAATGAGCCATTCGCAGTTTCGCCGTATAAAAGCTTATATAAC
Importance of organism and transcriptomes	###############################################################################################################################################################################################################################################################
Additional citations and references
Primary citation for organism's characterization, if available	doi:10.4319/lo.2011.56.1.0371
Latitude	41.54
Longitude	-70.61
Collection date	11-MAY-79
Sample collection site	Atlantic_Ocean
Other collection site info	Perch Pond, Falmouth, MA Nantucket Sound
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	19-DEC-11
Growth medium	f/2-Si amended seawater
Modifications to growth medium	f/2 for all nutrients except eliminate Si
Temperature (ºC)	15
Salinty (psu)	29.4
Light (µmol photons / m2 / sec)	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	106
Investigation type	Eukaryotes
Other experimental metadata available	not axenic; grown at 100uE/m2/s until peak exponential and moved to 8uE/m2/s