Mesodinium pulex SPMC105

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Environmental Data
Principle Investigator(s)	George McManus
External sample ID	MESPULEX
NCGR Sample ID	MMETSP0467
Sample accession number	CAM_SMPL_002570
Assembly accession number	CAM_ASM_000388
Combined Assembly Name	N/A
Genus	Mesodinium
Species	pulex
Strain	SPMC105
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	283647
18S rRNA	TCAACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACAA ACTAAATTATGGTGAAACTGCAAATGGCTCATTACAACGGTTATGACGTACCCGGGATTTCCGATGGATA TTTCCAGTAACACTAGAACTAATACATGTAACTGCGTTTATTAGTTTCAAACAGATCTGTCGCTGAGTCA TGGTAAACGGTTGATCGACTCGACGTCCCGGAACGGTTTCCGCCCCATCAGCTTCGATGGTTGGGTATTG GCCTACCATTGCAACAACGGGTAACGAGGAATTAGGGTTTGATTTCGGAGAGGGAGCCTGAGAAACGGCT ACCACATCTACGGATGGCAGCAGGCGCGCAAATTACCCAATGCAGACACTGTGAGGTAGTGACGAGAAAT AATAAAGCGGTGCTTTTGTATCGCTATTATAATGAGGAATACTTAAATGTCATTCCTATTAGCAACTGAT GGGAAAGTCTGGTGCCAGCAGCCGCGGTAATACCAGCTTCAGTAGCATATATTAAAATTGTTGCAGTTAA AACGTCCGTAGTCTGTACGTCGTCGTAGACCTTACTACAACTCTACATGTTCTTAACCGGGCGTATAGAA GTTTGTAGTTACTCCCTTGATCAAAATAGATTGCTCAAGGTAGACCAAGTCTTCATATCTTCACATGGAA TTGGGTGAGGGAACTTAAGAGTTTAAGTTTCTCGTCTTAGGATCATCTGGGGTCATTCGTACTCGTCCGC GAGAGGTGAAATTCTTGGACCGGACGAAGACGATCAGCAGCGAAAGCATCTGTCAGGGATGTTTTCTGCG ACAAAGGACGAAGGTCAGGGTATCGAAGACGATTAGATACCGTCGTAGTCCTGACTGTAAACTATGCCGA CTTGGGTCTTGCAGAGTAATTACTTTGTGAGACCCACACAGAAATGAAAGTGTATGGGTTCTGGGGGTAG TATGGACACAAGTCTGAAACTTGAAGGAATTGACGGAAGAGCACCATAAGACGTGGAGCTTGCGGCTTAA TTTGACTCAACGCGGGAAAACTTACCAGGTCAAGACATGGGCGTGATTGACAGGCTAATGACCTTTCTCG ATTCCTTGGATGGTGGTGCATGGTCGCACTTAGTTGGTGCAGTGATTTGTCAGGTTTATTCCGGTAACGA ACGAGACCTCGGCCTTGTAACTAGCTACGCTGCTTACAGGGACAATGACGGCTACGTCAAGGAAGTTCGA GGCAATTGCAGATCTGGAATGCCCTTAGATGTCCTGGGTCGCACGCGTGCTACAATGACGCCACGAACGA GTCCACGTGATGAAAGTCACGTGAAATCATCAATCGTCGTCTTAAGGGACAGTCTCTTGGAACTGTGGGA CTCTAACTAGGAATGTCTCGTAAGCACAAGTCAACATCTTGTGTTGATTACGTCCCTGCTCTTTGTACAC ACCGCCCGTCGCTCCTACCGATCGAGTGTTTAGATGAACTTGACGGACTCACGGAAGTTAAGTAAATCTG ATCACTTAGAGGAAGGAGAAGTCCTAACAAGGTTTCC
Importance of organism and transcriptomes	This heterotroph is a congener of Mesodinium rubrum, a ubiquitous and ecologically significant mixotroph. Transcriptome differences between M. pulex and M. rubrum may reveal key genes in the evolution of acquired phototrophy, an important mixotrophic nutritional mode in the sea.
Additional citations and references	Jakobsen, H. H, L. M. Everett, and S. L. Strom. ?Hydromechanical signaling between the ciliate Mesodinium pulex and motile protist prey.? Aquatic microbial ecology 44, no. 2 (2006): 197?206.
Primary citation for organism's characterization, if available	Tamar H (1992) Four marine species of Mesodinium (Ciliophora: Mesodiniidae). II. Mesodinium pulex CLAPH. & LACHM., 1858. Arch Protistenkd 141:284?303
Latitude	48.5
Longitude	-122.6
Collection date	01-JUN-03
Country	UNITED STATES
Experimental Data
Date of experiment	22-JUN-11
Growth medium	F/2 amended seawater
Modifications to growth medium	none
Temperature (ºC)	15
Salinty (psu)	30
pH	8
Light (µmol photons / m2 / sec)	100
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	900
Phosphate (μmol/L)	36
Trace elements (total) (nmol/L)	10000
Prey organism, if applicable (genus and species)	Heterocapsa rotundata MMETSP0503