Thalassiosira gravida GMp14c1

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Environmental Data
Principle Investigator(s)	Tatiana Rynearson
External sample ID	GMp14c1-Nlim
NCGR Sample ID	MMETSP0493
Sample accession number	CAM_SMPL_003120
Assembly accession number	CAM_ASM_000838
Combined Assembly Name	Thalassiosira-gravida-GMp14c1
Genus	Thalassiosira
Species	gravida
Strain	GMp14c1
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	420259
18S rRNA	AACCTGGTTGATCCTGCCAGTAGTCATACGCTCGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAACTAATTTTACTTTGAAAACTGCGAACGGCTCATTATATCAGTTATAGTTTATTTGATAGTCCCTTACTACTTGGATACCCGTAGTAATTCTAGAGCTAATACATGCATCAATACCCAACTGTTCGCGGAAGGGTAGTATTTATTAGGTATAGACCAACCCTCTTCGGAGGTGCTTTGGTGATTCATAATAACTTTTCGAATCGCATGGCTCCATGCCGGCGATGGATCATTCAAGTTTCTGCCCTATCAGCTTTGGATGGTAGTGTATTGGACTACCATGGCTTTAACGGGTAACGAATTGTTAGGGCAAGATTTCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTACAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCAGGAGTGACCGGTCACGCACTCTGTGCGTGAACTTGTGTTGTCTCTGGCCATCCTTGGGGAGATCCTGTTTGGCATTAAGTTGTCGGGCAGGGGATACCCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACTTCGGAACTATTTTGTTGGTTTGCGTTACGAAGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTTCGTTGTCAGAGGTGAAATTCTTGGATTTCCGAAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTCGGGATCGGAGGTTGTTTTTTGACTCCTTCGGCACCGTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAACCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGTTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGCCGCCTGCTAAATAGTTCTACTAATCGTTTTTGATTGGTTAAGAGCTTCTTAGAGGGACGTTCATTCTACAAGATGAAGGAAGATGGCGGCAATAACAGGTCTGTGATGCCCTTTGATGTCCTGGGCCGCACGCGCGTTACACTGATGCACTCAACGAGCATATAACCTTGGCCGAGAGGCCTGGGTAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAATCGCGGTTCATCAAACCGCAATGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTTCATTGGGGCCTGACCGCGAGAACTTGTCCGAACCTTATCATTTAGAGGAAGGTGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCA
Importance of organism and transcriptomes	The diatom Thalassiosira gravida is an important primary producer in polar and subpolar planktonic ecosystems in both the northern and southern hemispheres. Beyond its distribution and abundance very little is known about its physiology. Transcript data will provide new opportunities to examine the physiology and ecology of T. gravida.
Additional citations and references
Latitude	41.92732
Longitude	-67.85788
Depth (m)	10
Collection date	10-APR-11
Sample collection site	Atlantic_Ocean
Other collection site info	Gulf of Maine
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
Habitat description	Coastal waters
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	02-NOV-12
Growth medium	F/2 amended seawater
Modifications to growth medium	f/2 for all nutrients except nitrate, which was added at 10 uM
Temperature (ºC)	14
Salinty (psu)	35
pH	8.3
Light (µmol photons / m2 / sec)	178
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	10
Ammonium (μmol/L)	100
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	106
Trace elements (total) (nmol/L)	24.5
Investigation type	Eukaryotes