Skeletonema dohrnii SkelB

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Environmental Data
Principle Investigator(s)	Tatiana Rynearson
External sample ID	SkelB-N-limit
NCGR Sample ID	MMETSP0563
Sample accession number	CAM_SMPL_002834
Assembly accession number	CAM_ASM_000652
Combined Assembly Name	Skeletonema-dohrnii-SkelB
Genus	Skeletonema
Species	dohrnii
Strain	SkelB
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	267566
18S rRNA	CTAATGATAAGGTTTAGACAGGTTCTCGCGGTCAAGCCCCAATAAAGGAGCTAGACCAAAATCTCGAGTCTTCACCGGACCATTCAATCGGTAGGTGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCATTGCAGTTTGATGAACTGCGATTACTAGGAATTCCTCGTTCAAGATTAATAATTGCAATAATCTATCCCTATCACGATGCAAGTTAACAAGATTACCCAGGCCTCTCGGCCAAGGTTATATACTCGTTGAGTGCATCAGTGTAACGCGCGTGCGGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCCGCCATCTTCCTTCATCTTGTAGAATGAACGTCCCTCTAAGAAGACCTCGCCAATGAAAAGCCATTCGCGGGTCTATTTAGCAGGCGGCGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAACTCTCAATCTGTCAATCCTCACTATGTCTGGACCTGGTAAGTTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGTTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCATACAGTGCTGACGGAGTCAAAAAACAACCGCCAATCCTGAGTCGGCATAGTTTATGGTTAAGACTACGATGGTATCTAATCATCTTCGATCCCCTAACTTTCGTTCTTGATTAATGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTTCAGAAATCCAAGAATTTCACCTCTGACAATGAAATACGAATACCCCCAACTGTCCCTATTAATCATTACTTTGACTCGCAAACCAACAAAATAGACTCAAAGTCCTATCTTATTATTCCATGCTAATATATTCAACGGCATAAGCCTGCTTTAAACACTCTAATTTTTTCACAGTAAACGATGGTCATCCCCTTCCCGACAACTTAATGCCAAAAAGGATCTTACCAAGGATGGCCAGAATGACACAACTCACAAACAGAGTTTGTGGTCAGTCACTCCTGCCAGAAATCCAACTACGAGCTTTTTAACTGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGATACTCGATAAGGGATTTAAATTGTTCTCATTCCAATTGCCAGACCTGTAAAGGCCCGGCATTGTTATTTATTGTCACTACCTCCCTGTGTCAGGATTGGGTAATTTACGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTCTCTCAGGCTCCCTCTCCGAAATCTTGCCCTAACAATTCGTTACCCGTTAAAGCCATGGTAGTCCAATACACTACCATCCCAAGCTGATAGGGCAGAAACTTGAATGATCCATTGCCGACACGTGGTCATGCAATTCGAAAGGTTATTATGACTCACCAAATCAACTCCGAAGAGTGAAGGTTTAATACTAATAAACACGGCGGTTCCCGAGAGTTCCGCTTTGATGCATATATTAGCTCTAGAATTACTACGGTTATCCAAGTAGTGAGGGATTATCAAATAAACTATAACTGATATAATGAGCCGTCCGCAGTTTCGATAAAGTATCTTATACTTACACA
Importance of organism and transcriptomes	###############################################################################################################################################################################################################################################################
Additional citations and references	Borkman, D. G. and T. Smayda (2009). "Multidecadal (1959-1997) changes in Skeletonema abundance and seasonal bloom patterns in Narragansett Bay, Rhode Island, USA." Journal of Sea Research 61(1-2): 84-94; Kooistra, W. H. C. F., D. Sarno, S. Balzano, H. Gu, R. A. Andersen and A. Zingone (2008). "Global Diversity and Biogeography of Skeletonema Species (Bacillariophyta)." Protist 159(2): 177-193.
Latitude	41.57361
Longitude	-71.40861
Depth (m)	.5
Salinity (psu)	31.38
Temperature (ºC)	6.1
Collection date	22-NOV-10
Collection time	10:00 AM
Sample collection site	Atlantic_Ocean
Other collection site info	Narragansett Bay Long Term Plankton Times Series Station
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Not Applicable
Habitat description	Well mixed estuary
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	06-SEP-11
Growth medium	f/2 amended seawater
Modifications to growth medium	f/2 for all nutrients except nitrate
Temperature (ºC)	14
Salinty (psu)	36
pH	7.9
Light (µmol photons / m2 / sec)	180
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	10
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	106
Investigation type	Eukaryotes
Other experimental metadata available	Axenic