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Karenia brevis SP1

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Principle Investigator(s) Lisa Campbell
External sample IDSP1_27
NCGR Sample IDMMETSP0574
Sample accession numberCAM_SMPL_002844
Assembly accession numberCAM_ASM_000662
Combined Assembly NameKarenia-brevis-SP1
GenusKarenia
Speciesbrevis
StrainSP1
ClonalYes
AxenicNo
Prelim. NCBI Taxon ID156230
18S rRNA
Importance of organism and transcriptomesKarenia brevis is a planktonic dinoflagellate that produces harmful algal blooms almost every year in the Gulf of Mexico. Most strains of K. brevis produce a suite of toxic compounds including brevetoxins (2 main backbone types, PbTx-1 and -2) that are potent neurotoxins. The dominant life history stage of dinoflagellates is haploid, and the sexual stages and resting cysts (if any) are not well known for K. brevis. Most clonal cultures of K. brevis produce ~10-20 pg brevetoxin per cell and this is mostly PbTx-2 (90% of total toxin). The SP1 clone produces extremely low levels of brevetoxin (or, none). The toxin synthesis pathway and the function of brevetoxin in K. brevis is not known, therefore transcriptome data for SP1 clone in comparison with data from other clones (such as SP3, which produces mostly PbTx-1 and Wilson, which produces consistly high levels) will be informative. The comparative approach is valuable as no experimental genetic system (e.g. knock-out mutants) is available. Karenia brevis is a planktonic dinoflagellate that produces harmful algal blooms almost every year in the Gulf of Mexico. Most strains of K. brevis produce a suite of toxic compounds including brevetoxins (2 main backbone types, PbTx-1 and -2) that are potent neurotoxins. The dominant life history stage of dinoflagellates is haploid, and the sexual stages and resting cysts (if any) are not well known for K. brevis. Most clonal cultures of K. brevis produce ~10-20 pg brevetoxin per cell and this is mostly PbTx-2 (90% of total toxin). The SP1 clone produces extremely low levels of brevetoxin (or, none). The toxin synthesis pathway and the function of brevetoxin in K. brevis is not known, therefore transcriptome data for SP1 clone in comparison with data from other clones (such as SP3, which produces mostly PbTx-1 and Wilson, which produces consistly high levels) will be informative. The comparative approach is valuable as no experimental genetic system (e.g. knock-out mutants) is available.
Additional citations and referencesErrera, R. M., Bourdelais, A., Drennan, M. A., Dodd, E. B., Henrichs, D. W. & Campbell, L. 2010. Variation in brevetoxin and brevenal content among clonal cultures of Karenia brevis may influence bloom toxicity. Toxicon 55:195-203; Daugbjerg, N. G. Hansen, J. Larsen, and O. Moestrup. 2000. Phylogeny of some of the major genera of dinoflagellates based on ultrstructure and partial LSU rDNA sequence data, including the erection of three new general of unarmoured dinoflagellates. Phycologia 39(4): 302-317.
Environmental Data
Primary citation for organism's characterization, if availableHenrichs, D. W., Renshaw, M. A., Santamaria, C. A., Richardson, B., Gold, J. R. & Campbell, L. 2008. PCR amplification of microsatellites from single cells of Karenia brevis preserved in Lugol's iodine solution. Marine Biotechnology 10:122-27.
Collection date01-OCT-99
Sample collection siteGulf of Mexico
Other collection site infoSouth Padre Island
Sample material (e.g. "seawater," "sediment," etc.)Seawater
ENVO term for habitat - primary termAcquatic: marine
Habitat descriptioncoastal harmful algal bloom
Other environmental metadata availableHaplotype based on 18 microsatellite loci at http://oceanz.tamu.edu/~campbell/gmx_msat/
Other environmental metadata availableHaplotype based on 18 microsatellite loci at http://oceanz.tamu.edu/~campbell/gmx_msat/
Habitatmarine habitat
CountryUNITED STATES
Experimental Data
Date of experiment10-APR-11
Growth mediumL1 amended seawater
Modifications to growth mediumNA
Temperature (ºC)25
Salinty (psu)27
pH8.3
Light (µmol photons / m2 / sec)70
Day portion of day:night cycle in hours12
Night portion of day:night cycle in hours12
Nitrate (μmol/L)882
Phosphate (μmol/L)36.2
Trace elements (total) (nmol/L)25
Prey organism, if applicable (genus and species)NA
Investigation typeEukaryotes
Other experimental metadata availablerapid shift to 27 psu