Skeletonema grethea CCMP 1804

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Environmental Data
Principle Investigator(s)	Tatiana Rynearson
External sample ID	CCMP1804-replete- day
NCGR Sample ID	MMETSP0578
Sample accession number	CAM_SMPL_002845
Assembly accession number	CAM_ASM_000663
Combined Assembly Name	N/A
Genus	Skeletonema
Species	grethea
Strain	CCMP 1804
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	267565
18S rRNA	ACCTGGTTGATCCTGCCAGTAGTCATACGCTCGTCTCAAAGATTAAGCCATGCATGTGTAATTATAAGATACTTTATCGAAACTGCGGACGGCTCATTATATCAGTTATAGTTTATTTGATAATCCCTCACTACTTGGATAACCGTAGTAATTCTAGAGCTAATATATGCATCAAAGCGGAACTCTCGGGAACCGCCGTGTTTATTAGTATTAAACCTTCACTCTTCGGAGTTGATTTGGTGAGTCATAATAACCTTTCGAATTGCATGCACATGTTGCGGCAATGGATCATTCAAGTTTCTGCCCTATCAGCTTGGGATGGTAGTGTATTGGACTACCATGGCTTTAACGGGTAACGAATTGTTAGGGCAAGATTTCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTACAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCAGGAGTGACCGGCCACACACACTGTGTGTGAGTTGTGTCATTCTGGCCATCCTTGGTGAGATCCTGTTTGGCATTAAGTTGTCGGGCAGGGGATAACCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACTTTGAGTCTATTTTGTTGGTTTGCGAGTCAAAGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTCTGAAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTCAGGATTGGCGGTTGTTTTTTGACTCCGTCAGCACTGTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAACCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGTTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGCCGCCTGCTAAATAGACCCGCGAATAGCTTTTTATTGGCGAGGTCTTCTTAGAGGGACGTTCATTCTACAAGATGAAGGAAGATGGCGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCCGCACGCGCGTTACACTGATGCACTCAACGAGCATATAACCTTGGCCGAGAGGCCTGGGTAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAATCGCAGTTCATCAAACTGCAATGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGAGTCGAGATTGTGGATTAGCTCCTTTATTGGGGCTTTTCCGCGAGAACCTCTCCAAACCTTATCATTTAGAGGAAGGTGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATCAA
Importance of organism and transcriptomes	Species in the genus Skeletonema form a group of important bloom-forming diatoms widely distributed along coasts and in estuaries
Additional citations and references	###############################################################################################################################################################################################################################################################
Primary citation for organism's characterization, if available	Sarno, D., Kooistra, W.H.C.F., Medlin, L.K., Percopo, I. & Zingone, A. (2005). Diversity in the genus Skeletonema (Bacillariophyceae): II. An assessment of the taxonomy of S. costatum-like species, with the description of four new species. Journal of Phycology 41: 151-176.
Latitude	41.4331
Longitude	-71.455
Depth (m)	.5
Collection date	01-AUG-95
Sample collection site	Narragansett, RI
Other collection site info	URI-GSO Dock
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Not Applicable
Habitat description	Coastal Waters
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	14-JAN-12
Growth medium	f/2 amended seawater
Temperature (ºC)	14
Salinty (psu)	36
pH	7.9
Light (µmol photons / m2 / sec)	180
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	883
Phosphate (μmol/L)	36.3
Silicate (μmol/L)	107
Investigation type	Eukaryotes
Other experimental metadata available	Axenic