Skeletonema japonicum CCMP2506

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Environmental Data
Principle Investigator(s)	Tatiana Rynearson
External sample ID	CCMP2506-replete- day
NCGR Sample ID	MMETSP0593
Sample accession number	CAM_SMPL_002854
Assembly accession number	CAM_ASM_000672
Combined Assembly Name	N/A
Genus	Skeletonema
Species	japonicum
Strain	CCMP2506
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	267983
18S rRNA	TGTGTAAGTATAAGATACTTTATCGAAACTGCGGACGGCTCATTATATCAGTTATAGTTTATTTGATAATCCCTCACTACTTGGATAACCGTAGTAATTCTAGAGCTAATATATGCATCAAAGCGGAACTCTCGGGAACCGCCGTGTTTATTAGTATTAAACCTTCACTCTTCGGAGTTGATTTGGTGAGTCATAATAACCTTTCGAATTGCATGCACATGTTGCGGCAATGGATCATTCAAGTTTCTGCCCTATCAGCTTGGGATGGTAGTGTATTGGACTACCATGGCTTTAACGGGTAACGAATTGTTAGGGCAAGATTTCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTACAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCAGGAGTGACCGGCCACACACACTGTGCGTGAGTTGCGTCATTCTGGCCATCCTTGGTGAGATCCTGTTTGGCATTAAGTTGTCGGGCAGGGGATAACCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACTTTGAGTCTATTTTGTTGGTTTGCGAGTCAAAGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTCTGAAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTCAGGATTGGCGGTTGTTTTTTGACTCCGTCAGCACTGTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAACCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGTTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGCCGCCTGCTAAATAGACCCGCGAATAGCTTTTTATTGGCGAGGTCTTCTTAGAGGGACGTTCATTCTACAAGATGAAGGAAGATGGCGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCCGCACGCGCGTTACACTGATGCACTCAACGAGCATATAACCTTGGCCGAGAGGCCTGGGTAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAATCGCAGTTCATCAAACTGCAATGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGAGTCGAGATTGTGGATTAGCTCCTTTATTGGGGCTTATCCGCGAGAACCTCTCCAAACCT
Importance of organism and transcriptomes	Species in the genus Skeletonema form a group of important bloom-forming diatoms widely distributed along coasts and in estuaries
Additional citations and references	###############################################################################################################################################################################################################################################################
Primary citation for organism's characterization, if available	Sarno, D., Kooistra, W.H.C.F., Medlin, L.K., Percopo, I. & Zingone, A. (2005). Diversity in the genus Skeletonema (Bacillariophyceae): II. An assessment of the taxonomy of S. costatum-like species, with the description of four new species. Journal of Phycology 41: 151-176.
Latitude	34.3
Longitude	132.4
Depth (m)	.5
Collection date	01-OCT-02
Sample collection site	Seto Inland Sea
Other collection site info	Hiroshima Bay
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Not Applicable
Habitat description	Coastal Waters
Habitat	marine habitat
Country	JAPAN
Experimental Data
Date of experiment	09-DEC-11
Growth medium	f/2 amended seawater
Temperature (ºC)	14
Salinty (psu)	36
pH	7.9
Light (µmol photons / m2 / sec)	180
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	883
Phosphate (μmol/L)	36.3
Silicate (μmol/L)	107
Investigation type	Eukaryotes
Other experimental metadata available	Axenic