Skeletonema menzelii CCMP793

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Environmental Data
Principle Investigator(s)	Tatiana Rynearson
External sample ID	CCMP793-replete
NCGR Sample ID	MMETSP0603
Sample accession number	CAM_SMPL_002864
Assembly accession number	CAM_ASM_000682
Combined Assembly Name	Skeletonema-menzelii-CCMP793
Genus	Skeletonema
Species	menzelii
Strain	CCMP793
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	216823
18S rRNA	CATGCATGTGTAAGTATAAGATACTTTATCGAAACTGCGGACGGCTCATTATATCAGTTATAGTTTATTTGATAATCCCTCACTACTTGGATAACCGTAGTAATTCTAGAGCTAATATATGCATCAAAGCGGAACTCTCGGGAACCGCCGTGTTTATTAGTATTAAACCTTCACTCTTCGGAGTTGATTTGGTGAGTCATAATAACCTTTCGAATTGCATGCACATGTTGCGGCAATGGATCATTCAAGTTTCTGCCCTATCAGCTTGGGATGGTAGTGTATTGGACTACCATGGCTTTAACGGGTAACGAATTGTTAGGGCAAGATTTCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTACAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCAGGAGTGACCGGCCACACACACCGTGTGTGAGTTGTGCATTCTGGCCATCCTTGGTGAGATCCTGTTTGGCATTAAGTTGTCGGGCAGGGGATAACCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACTTTGAGTCTATTTTGTTGGTTTGCGAGTCAAAGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTCTGAAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTCAGGATTGGCGGTTGTTTTTTGACTCCGTCAGCACTGTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAACCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGTTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCCCCGCCTGCTAAATAAATGGGTGAATGAATTTTCATTGACTACATTTTCTTAGAGGGACGTTCGCTCTACAAGGCGAAGGAAGATGGGGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCCGCACGCGCGTTACACTGATGCACTCAACGAGCATATAACCTTGGCCGAGAGGCCTGGGTAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAATCGCAGTTCATCAAACTGCAGTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGACTCGAGATTGTGGTCTAGCTCCTTCATTGGGGCTTGACCGCGAGAACCTGTCTAAAC
Importance of organism and transcriptomes	The diatom genus Skeletonema is an abundant component of many estuarine and coastal environments around the globe. Along the Northeast coast of the US, this planktonic genus is a major contributor to primary productivity. Transcriptomes of S. menzelii generated here will be used to better understand the bloom dynamics of species in the genus Skeletonema.
Additional citations and references	Kooistra, W. H. C. F., D. Sarno, S. Balzano, H. Gu, R. A. Andersen and A. Zingone (2008). "Global Diversity and Biogeography of Skeletonema Species (Bacillariophyta)." Protist 159(2): 177-193.
Latitude	41.566
Longitude	-70.5842
Collection date	18-JUL-82
Sample collection site	Atlantic_Ocean
Other collection site info	Perch Pond, Falmouth, Massachusetts USA
Sample material (e.g. "seawater," "sediment," etc.)	seawater
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Not Applicable
Habitat description	Coastal salt pond
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	26-SEP-11
Growth medium	f/2 amended seawater
Modifications to growth medium	f/2 for all nutrients
Temperature (ºC)	14
Salinty (psu)	36
pH	7.9
Light (µmol photons / m2 / sec)	180
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	106
Investigation type	Eukaryotes
Other experimental metadata available	Axenic