Marine Microbial Eukaryote Transcriptome Sequencing Project
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Extubocellulus spinifer CCMP396
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| Principle Investigator(s) | Sarah Smith |
|---|---|
| External sample ID | E.spinifer_Nocodazole |
| NCGR Sample ID | MMETSP0699 |
| Sample accession number | CAM_SMPL_002827 |
| Assembly accession number | CAM_ASM_000645 |
| Combined Assembly Name | Extubocellulus-spinifer-CCMP396 |
| Genus | Extubocellulus |
| Species | spinifer |
| Strain | CCMP396 |
| Clonal | Yes |
| Axenic | Yes |
| Prelim. NCBI Taxon ID | 265572 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Extubocellulus spinifer is an unusual tiny benthic centric diatom that is common in the marine coastal environment. Little is known about the evolution, biology, or ecological significance of this diatom. |
| Additional citations and references | |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Hasle, G.R., Stosch, H.A. von & Syvertsen, E.E. (1983). Cymatosiraceae, a new diatom family. Bacillaria 6: 9-156. |
| Latitude | 31.3172 |
| Longitude | 113.56 |
| Collection date | 01-JAN-68 |
| Sample collection site | Gulf of California (Sea of Cortes) |
| ENVO term for habitat - primary term | Acquatic: marine |
| Habitat | marine habitat |
| Country | MEXICO |
| Experimental Data | |
| Date of experiment | 10-SEP-11 |
| Growth medium | ASW |
| Modifications to growth medium | Cells grown exponentially in ASW were harvested by centrifugation and re-suspended in fresh ASW with 2.5mg L-1 nocodazole |
| Temperature (ºC) | 20 |
| pH | 8 |
| Light (µmol photons / m2 / sec) | 200 |
| Day portion of day:night cycle in hours | 24 |
| Nitrate (μmol/L) | 2.97 |
| Phosphate (μmol/L) | .2 |
| Silicate (μmol/L) | .42 |
| Trace elements (total) (nmol/L) | 19.73 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | cell concentration, neutral lipid content (fluorometrically determined with Bodipy) |