Marine Microbial Eukaryote Transcriptome Sequencing Project
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Asterionellopsis glacialis CCMP134
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| Principle Investigator(s) | Sarah Smith |
|---|---|
| External sample ID | A.glacialis_Nocodazole |
| NCGR Sample ID | MMETSP0708 |
| Sample accession number | CAM_SMPL_002838 |
| Assembly accession number | CAM_ASM_000656 |
| Combined Assembly Name | Asterionellopsis-glacialis-CCMP134 |
| Genus | Asterionellopsis |
| Species | glacialis |
| Strain | CCMP134 |
| Clonal | Yes |
| Axenic | No |
| Prelim. NCBI Taxon ID | 33640 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Asterionellopsis glacialis is a chain-forming pennate diatom commonly found in the cold to temperate ocean waters globally. |
| Additional citations and references | |
| Environmental Data | |
| Latitude | 32.655 |
| Longitude | 117.14 |
| Collection date | 01-MAY-63 |
| Sample collection site | Pacific_Ocean |
| Other collection site info | San Diego Bay |
| ENVO term for habitat - primary term | Acquatic: marine |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 24-AUG-11 |
| Growth medium | NEPC |
| Modifications to growth medium | Cells grown exponentially in NEPC were harvested by centrifugation and re-suspended in fresh NEPC with 2.5mg L-1 nocodazole |
| Temperature (ºC) | 20 |
| pH | 8 |
| Light (µmol photons / m2 / sec) | 200 |
| Day portion of day:night cycle in hours | 24 |
| Nitrate (μmol/L) | .55 |
| Phosphate (μmol/L) | .01 |
| Silicate (μmol/L) | .11 |
| Trace elements (total) (nmol/L) | 16.33 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | cell concentration, neutral lipid content (fluorometrically determined with Nile Red) |