Marine Microbial Eukaryote Transcriptome Sequencing Project
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Amphiprora sp. CCMP467
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| Principle Investigator(s) | Sarah Smith |
|---|---|
| External sample ID | A.sp_Nocodazole |
| NCGR Sample ID | MMETSP0727 |
| Sample accession number | CAM_SMPL_002709 |
| Assembly accession number | CAM_ASM_000527 |
| Combined Assembly Name | Amphiprora-sp |
| Genus | Amphiprora |
| Species | sp. |
| Strain | CCMP467 |
| Clonal | Yes |
| Axenic | No |
| Prelim. NCBI Taxon ID | 265536 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Amphiprora sp. is an unusual benthic pennate diatom that is common in the marine environment but has not been well-represented in the published literature. |
| Additional citations and references | |
| Environmental Data | |
| ENVO term for habitat - primary term | Acquatic: marine |
| Habitat | marine habitat |
| Experimental Data | |
| Date of experiment | 01-DEC-11 |
| Growth medium | NEPC |
| Modifications to growth medium | NEPC for all nutrients + 2.5mg L-1 of the microtubule inhibitor nocodazole |
| Temperature (ºC) | 20 |
| Salinty (psu) | 30 |
| pH | 8 |
| Light (µmol photons / m2 / sec) | 200 |
| Day portion of day:night cycle in hours | 12 |
| Night portion of day:night cycle in hours | 12 |
| Nitrate (μmol/L) | .55 |
| Phosphate (μmol/L) | .01 |
| Silicate (μmol/L) | .11 |
| Trace elements (total) (nmol/L) | 16.33 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | growth, neutral lipid content (fluorometrically determined with Nile Red or Bodipy) |