Marine Microbial Eukaryote Transcriptome Sequencing Project
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Nitzschia punctata CCMP561
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| Principle Investigator(s) | Sarah Smith |
|---|---|
| External sample ID | N.punctata_Nocodazole |
| NCGR Sample ID | MMETSP0747 |
| Sample accession number | CAM_SMPL_002750 |
| Assembly accession number | CAM_ASM_000568 |
| Combined Assembly Name | Nitzschia-punctata-CCMP561 |
| Genus | Nitzschia |
| Species | punctata |
| Strain | CCMP561 |
| Clonal | Yes |
| Axenic | No |
| Prelim. NCBI Taxon ID | 913974 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Nitzschia punctata is an unusual benthic pennate diatom that tolerates a wide range of salinity regimes. It readily accumulates intracellular droplets of triacylglycerol. |
| Additional citations and references | |
| Environmental Data | |
| ENVO term for habitat - primary term | Acquatic: marine |
| Habitat | marine habitat |
| Experimental Data | |
| Date of experiment | 01-DEC-11 |
| Growth medium | ASW |
| Modifications to growth medium | ASW for all nutrients + 2.5mg L-1 of the microtubule inhibitor nocodazole |
| Temperature (ºC) | 20 |
| Salinty (psu) | 30 |
| pH | 8 |
| Light (µmol photons / m2 / sec) | 200 |
| Day portion of day:night cycle in hours | 24 |
| Nitrate (μmol/L) | 2.97 |
| Phosphate (μmol/L) | .2 |
| Silicate (μmol/L) | .42 |
| Trace elements (total) (nmol/L) | 19.73 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | growth, neutral lipid content (fluorometrically determined with Nile Red or Bodipy) |