Percolomonas cosmopolitus AE-1 (ATCC 50343)

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Environmental Data
Principle Investigator(s)	Alastair Simpson
External sample ID	Percolomonas50343
NCGR Sample ID	MMETSP0758
Sample accession number	CAM_SMPL_002771
Assembly accession number	CAM_ASM_000589
Combined Assembly Name	N/A
Genus	Percolomonas
Species	cosmopolitus
Strain	AE-1 (ATCC 50343)
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	63605
18S rRNA	CCATGCAATTGTCAGAAAATCATGTTCAGTCGCAAGATTGAAAATTGTGA ATCTGAGACTCGCTCCTTAAACAAATCCTAGTGCATCCTTTAGTTTGAAT ATGTTGTATAACATGTTTATTAAATGGATACCTGACCTAACTGGTCAGTT AATACATGATAACTTTAGTAGTCATCTATTTTTAAATTGAATTTTGGTGA CTAATATTGGAAACTTTTTCGGAAGTGGAAGATAYGCARACCTACAGAAC AGGGTTTACCTGATATGAGTYTTTTGATTCTTTGGAATCAAGAAATTTAA CTGAAGGCCTTAAGGAACAAGGATTTAAGGATTAGTTAGTTGGTACGGTA GTGTCCTATCAAGACTATAATCCTTACAGAGAAATTATCGTTTGATTTCG GAGAGAGAGCCTTAAAAACCGCTATCACRTCTAAGGAAGGCAGCAGGCGC GCAAATTACCCAATATCAATTCGATGAGGTAGTGACAAGACGTAATGACC TCCCACTTTTATGAGGGTTATCCGCAAGGATATTACAATGGTCTGAAATT AAAATTCTCARAGAGAAACGACTAGAGGAAAAGTCTGGTGCCAGCACCCG CGGTAATTCCAGCTCTAGTAGTATATATTAATGTTGTTGTATTTAAAACG TTCGTAGTTTACTTAAGTTATTTTTTRTTATTGTCGGTTTATTAACTCTA CTTTTATTTTATGTGAATAAARTAAAGGGTTCACCTTCGGGTCGATTTAA GGGAAGATAACTCTTTATTGGTGAGAAACAATGTAGAGCTATTTATCAAA GCATAAATAGTATCTTATTGTGGATCATGGCAATAATTGAAAAGTAACGT TTAGAAGTGAGAAAAAATCTATATGCTTCATGTAAGCTAAACCCGTTCTA ATCTTCGGAAAAGAACAATTTTTTGCTTGAAAGTTATAGCTTGCTATCCT TTAAAATTGTATGGAAGTTATTATCCTTAGAGGTAATAAAAGAAATACAG TGTTTATAATGGAACTCATGTCAAGTTAACAGTATTACTAAGATAGAGGT GAAATTCAATAACCCTAGTAAGACTACCTAATGCTAACGCAGTTAACAAG ACTGGCTTCATCAATCAGGAACTAAAGTTAGAGGATCGAAGATGATTAGA TACCGTTGTAGTTCTAACCGTAAACTATTTCGACAGAAAATTGGTRTTAA ATATTTTTAAAGATAAYATCAGAGTTCAAGGGAAACCTTAAGTTTTTGGA ATATAGGGGGAATACGAACGCAAGTTTGAAACTTAAAAGAATTGACGGAA AAGCACCACCAGGTGTGGATTCTGCGGCTTAATTCGACTCAACGCGGGGA AACTTGCCAAGTTAGGATACAAGTAGGATTGACAGAGTAAAGCTCTTTCT TGATTTTGTAGTTGGTGGTGCATGGCCATTCTTAGTTCGTGGAGTGATTT GTCTTGTTAATTCAGATAACGAGCGAGACCTAAGCCAATATTTTTTATGC TTTTTATTTTCTTGTAAAATAGAATGACAAAAACTTCTATTCAAAAATCT AGAAATAGTSGAATAYGAAGGTTAGTTATTGGGATACCTTATTTTTTATT ACCGTTCAAAGGAAGGTGAAACGTTAGGCAATAATAGGTCTGTGATGCCC TTAGATATCTTGGAATGCACGCGGGATACAATAATTAAGATAATAAGTTT ATTTTAATTTACATTGTGTTTTTTGCATTAATTGTAAGTAAAACCTGTTT YGAAAGGAAWGGGAAATCTCTCCAAATYTTAATTTTGACAAGGATTGTCT CKTGTAACAGAGACATGAAAGAGGAATACMTAGTAGGCGTGAGTCAACAC CTCGCACCGATTAAGTCCCTGCTTTTTGTACACACCGCCCGTCGCGATCA AGGAACACGATTGAAGTCATGAAGCTTACGGATAGATTACATTAGTGATT TAAAAGTAAACTGAAT
Importance of organism and transcriptomes	The small free-living flagellate Percolomonas cosmopolitus (Excavata: Heterolobosea) is one of most diverse morphospecies known, according to rRNA sequences. Transcriptome data from two disparate isolates of P. cosmopolitus will give a first view of the differences at the functional level within this morphospecies. Furthermore, Percolomonas is an aerobic mesophile that only has a flagellate stage, and as such is an important comparator organism for studies of anaerobic, thermophilic and halophilic Heterolobosea, as well as well-studied amoeboflagellate forms in which the genomic underpinnings of motility are studied (Naegleria).
Additional citations and references	PMID: 19120795
Primary citation for organism's characterization, if available	PMID: 15537093
ENVO term for habitat - primary term	Other
Habitat description	Isolated from a Marine Aquarium in Rockville, MD, USA
Habitat	other
Country	UNITED STATES
Experimental Data
Date of experiment	02-JUN-11
Growth medium	artificial seawater: NaCl (24.72 g/L), KCl (6.7 g/L), CaCl2 (1.03 g/L), MgCl2 (2.18 g/L), MgSO4 (3.07 g/L), NaHCO3 (0.18 g/L)
Temperature (ºC)	22
Salinty (psu)	32
pH	8.4
Prey organism, if applicable (genus and species)	Enterobacter aerogenes
Investigation type	Eukaryotes