Marine Microbial Eukaryote Transcriptome Sequencing Project
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Pseudo-nitzschia fraudulenta WWA7
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| Principle Investigator(s) | Bethany Jenkins |
|---|---|
| External sample ID | Si-limited 390 ppm |
| NCGR Sample ID | MMETSP0851 |
| Sample accession number | CAM_SMPL_002813 |
| Assembly accession number | CAM_ASM_000631 |
| Combined Assembly Name | Pseudo_nitzschia-fradulenta-WWA7 |
| Genus | Pseudo-nitzschia |
| Species | fraudulenta |
| Strain | WWA7 |
| Clonal | Yes |
| Axenic | No |
| Prelim. NCBI Taxon ID | 183588 |
| 18S rRNA | |
| Importance of organism and transcriptomes | Many pennate diatoms in the genus Pseudo-nitzschia have the potential to produce a potent neurotoxin, domoic acid (DA), which bioaccumulates through trophic transfer and can consequently devastate marine mammal and bird populations. To date, nothing is known regarding the genetic basis for DA production, although there are many physiological triggers of DA production including limitation by macronutrients (e.g. silicate (Si)). Recent studies have shown that CO2 levels likely to be encountered at the end of this century further exacerbate DA production in Si-limited cultures of Pseudo-nitzschia fraudulenta, suggesting an interesting co-regulation of DA synthesis by both Si limitation and increasing CO2 levels. Comparison of the gene complement expressed in Pseudo-nitzschia fraudulenta in DA producing conditions with the draft genome of Pseudo-nitzschia multiseries, a species that has also been shown to produce DA, should help elucidate genes unique to the Pseudo-nitzschia genus and those important for DA production. Data from these transcriptomes will also be relevant for interpreting how diatoms are likely to respond to both environmental fluctuations of nutrients (in this case Si) and CO2 levels corresponding to present and future climates. |
| Additional citations and references | |
| Environmental Data | |
| Latitude | 34.0847 |
| Longitude | -119.05 |
| Depth (m) | 1 |
| Salinity (psu) | 35 |
| Temperature (ºC) | 14 |
| Collection date | 27-MAR-10 |
| Collection time | 06:30 AM |
| Sample collection site | Pacific_Ocean |
| Sample material (e.g. "seawater," "sediment," etc.) | seawater |
| ENVO term for habitat - primary term | Acquatic: marine |
| ENVO term for habitat - secondary term | Other |
| Habitat description | small cove with cobblestone/sand bottom |
| Other environmental metadata available | no other types of environmental metadata available |
| Other environmental metadata available | no other types of environmental metadata available |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 09-AUG-06 |
| Growth medium | f/2 enriched seawater |
| Modifications to growth medium | f/2 for all nutirents except the final concentration of sodium metasilicate was 10.6 uM |
| Temperature (ºC) | 16 |
| Salinty (psu) | 35 |
| pH | 8.2 |
| Light (µmol photons / m2 / sec) | 90 |
| Day portion of day:night cycle in hours | 12 |
| Night portion of day:night cycle in hours | 12 |
| Nitrate (μmol/L) | 643 |
| Phosphate (μmol/L) | 32 |
| Silicate (μmol/L) | 10.6 |
| CO2 (ppm) | 390 |
| Investigation type | Eukaryotes |