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Pseudo-nitzschia fraudulenta WWA7


Principle Investigator(s) Bethany Jenkins
External sample IDSi-limited 390 ppm
Sample accession numberCAM_SMPL_002813
Assembly accession numberCAM_ASM_000631
Combined Assembly NamePseudo_nitzschia-fradulenta-WWA7
Prelim. NCBI Taxon ID183588
18S rRNA
Importance of organism and transcriptomesMany pennate diatoms in the genus Pseudo-nitzschia have the potential to produce a potent neurotoxin, domoic acid (DA), which bioaccumulates through trophic transfer and can consequently devastate marine mammal and bird populations. To date, nothing is known regarding the genetic basis for DA production, although there are many physiological triggers of DA production including limitation by macronutrients (e.g. silicate (Si)). Recent studies have shown that CO2 levels likely to be encountered at the end of this century further exacerbate DA production in Si-limited cultures of Pseudo-nitzschia fraudulenta, suggesting an interesting co-regulation of DA synthesis by both Si limitation and increasing CO2 levels. Comparison of the gene complement expressed in Pseudo-nitzschia fraudulenta in DA producing conditions with the draft genome of Pseudo-nitzschia multiseries, a species that has also been shown to produce DA, should help elucidate genes unique to the Pseudo-nitzschia genus and those important for DA production. Data from these transcriptomes will also be relevant for interpreting how diatoms are likely to respond to both environmental fluctuations of nutrients (in this case Si) and CO2 levels corresponding to present and future climates.
Additional citations and references
Environmental Data
Depth (m)1
Salinity (psu)35
Temperature (ºC)14
Collection date27-MAR-10
Collection time06:30 AM
Sample collection sitePacific_Ocean
Sample material (e.g. "seawater," "sediment," etc.)seawater
ENVO term for habitat - primary termAcquatic: marine
ENVO term for habitat - secondary termOther
Habitat descriptionsmall cove with cobblestone/sand bottom
Other environmental metadata availableno other types of environmental metadata available
Other environmental metadata availableno other types of environmental metadata available
Habitatmarine habitat
Experimental Data
Date of experiment09-AUG-06
Growth mediumf/2 enriched seawater
Modifications to growth mediumf/2 for all nutirents except the final concentration of sodium metasilicate was 10.6 uM
Temperature (ºC)16
Salinty (psu)35
Light (µmol photons / m2 / sec)90
Day portion of day:night cycle in hours12
Night portion of day:night cycle in hours12
Nitrate (μmol/L)643
Phosphate (μmol/L)32
Silicate (μmol/L)10.6
CO2 (ppm)390
Investigation typeEukaryotes