Heterosigma akashiwo CCMP 452

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Environmental Data
Principle Investigator(s)	Rose Ann Cattolico
External sample ID	Cyst formation: vegetative phase hr 6 day 0
NCGR Sample ID	MMETSP0894_2
Sample accession number	CAM_SMPL_002722
Assembly accession number	CAM_ASM_000540
Combined Assembly Name	Heterosigma-akashiwo-CCMP452
Genus	Heterosigma
Species	akashiwo
Strain	CCMP 452
Clonal	No
Axenic	Yes
Prelim. NCBI Taxon ID	536046
18S rRNA	CATTATATCAGTTATAGTTTATTTGATAGTCCCTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCATCAACTCCCAACTGCTTCGGCGGACGGGATGTATTTATTAGATGGAAACCAATACCGAGTTTTCTCGGTTTTTGTGGTGAATCATAGTAACTGTGCGAATCGACTTCGGTCGATGGTTCATTCAAGTTTCTGCCCTATCAGCTTCGGATGGTAGGGTATTGGCCTACCATGGCTTTAACGGGTAACGGAGAATTGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACATAGGGAGGTAGTGACAATAAATAACAATGCTAGGCTTTTAAAGTCCGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGGAACCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGTGGAGGTGATCGGTCGGCCTCGCAAGGGGTCTGCACCTGTATTGTCCTTTGCCATCCTTCAGGAAGGCGCTTCTTGTATTAACTTACGGGTTGCGAACTCCTGATCTTTTACTGTGAAAAAATTAGAGTGTTCAAAGCAGGCTTAGGCTGTTGAATACATTAGCATGGAATAATGAGATAGGGCCTTGGTGGTTTTCTATTTTGTTGGTTTGCACGCCAAGGTAATGATTAATAGGGATAGTTGGGGGTATTCGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATGGAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTAGGGATTGGCGGTCGTTACCTAGACTCCGTCAGCACCTTCCGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCCCCGCCTGCTAAATAGTGTCGGTAATGCTTCTGCATTGCCGTTTCTACTTCTTAGAGGGACTTTCGGTGACTAACCGAAGGAAGTTGGGGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCATGCAACGAGTAACGACCTTTGCCGGAAGGCACGGGTAATCTTTTGAACGTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAAACGCGAGTCATCAGCTCGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTTATTGTGACTTGCCGAGGGAAGTTATTTAAACCTCAT
Importance of organism and transcriptomes	The Heterosigma akashiwo strain CCMP 452 was initially isolated in Conover Maine (1952). This ~10 um alga rapidly divides as a vegetative cell and can form overwintering cysts that initiate massive, destructive blooms that impact eco-cohort survival at every trophic level. H. akashiwo is found in temperate and sub-tropical waters world-wide and often serves as the primary producer in coastal ecosystems. Intense studies of H. akashiwo for at least 40 years have generated a huge literature concerning the physiology, biochemistry and molecular biology of this organism.
Additional citations and references	DOI: 10.1021/bi00527a023, DOI: 10.1127/1438-9134/2010/0136-0261, PMID: PMC1185438, PMID: PMC2410131, DOI: 10.1046/j.1529-8817.2002.0187.x, PMID: PMC297543, PMID: PMC1074857, PMID: PMC386978, PMID: PMC1056607
Primary citation for organism's characterization, if available	PMID: PMC542060
Latitude	41
Longitude	-73
Collection date	01-JAN-52
Sample collection site	Long Island Sound
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	31-MAY-11
Growth medium	O-3 Medium doi:10.1016/0003-2697(78)90546-8
Temperature (ºC)	20
Salinty (psu)	30
pH	8.3
Light (µmol photons / m2 / sec)	40
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	7520
Ammonium (μmol/L)	179
Phosphate (μmol/L)	365
Total Fe (nmol/L)	7240000
Trace elements (total) (nmol/L)	12800000
Investigation type	Eukaryotes
Other experimental metadata available	60 rpm agitation