Thalassiosira rotula GSO102

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Environmental Data
Principle Investigator(s)	Tatiana Rynearson
External sample ID	GSO102-replete
NCGR Sample ID	MMETSP0913
Sample accession number	CAM_SMPL_003109
Assembly accession number	CAM_ASM_000827
Combined Assembly Name	Thalassiosira-rotula-GSO102
Genus	Thalassiosira
Species	rotula
Strain	GSO102
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	49265
18S rRNA	TGTCTAAGTATAACTCTTTTACTTTGAAAACTGCGAACGGCTCATTATATCAGTTATAGTTTATTTGATAGTCCCTTACTACTTGGATACCCGTAGTAATTCTAGAGCTAATACATGCATCAATACCCAACTGTTCGCGGAAGGGTAGTATTTATTAGGTATAGACCAACCCTCTTCGGAGGTGCTTTGGTGATTCATAATAACTTTTCGAATCGCATGGCTCCATGCCGGCGATGGATCATTCAAGTTTCTGCCCTATCAGCTTTGGATGGTAGTGTATTGGACTACCATGGCTTTAACGGGTAACGAATTGTTAGGGCAAGATTTCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTACAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCAGGAGTGACCGGTCACGCACTCTGTGCGTGAACTTGTGTTGTCTCTGGCCATCCTTGGGGAGATCCTGTTTGGCATTAAGTTGTCGGGCAGGGGATACCCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACTTCGGAACTATTTTGTTGGTTTGCGTTACGAAGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTTCGTTGTCAGAGGTGAAATTCTTGGATTTCCGAAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTCGGGATCGGAGGTTGTTTTTTGACTCCTTCGGCACCGTATGAGAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAACCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGTTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGCCGCCTGCTAAATAGTTCTACTAATCGTTTTTGATTGGTTAAGAGCTTCTTAGAGGGACGTTCATTCTACAAGATGAAGGAAGATGGCGGCAATAACAGGTCTGTGATGCCCTTTGATGTCCTGGGCCGCACGCGCGTTACACTGATGCACTCAACGAGCATATAACCTTGGCCGAGAGGCCTGGGTAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAATCGCGGTTCATCAAACCGCAATGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGCTTGGCTCCTTCATTGGGGCCTGACCGCGAGAACTTGTCCGAA
Importance of organism and transcriptomes	Thalassiosira rotula is a globally important, bloom forming species found in diverse ecosystems throughout the world. This species is comprised of at least three lineages that are differentially distributed throughout the globe. Whittaker, KA, Rignanese DR, Olson RJ, Rynearson TA. 2012. Molecular subdivision of the marine diatom Thalassiosira rotula in relation to geographic distribution, genome size, and physiology. BMC Evolutionary Biology. 12:209.
Additional citations and references
Primary citation for organism's characterization, if available	Meunier A: Microplancton des Mers de Berents et de Kara. Duc d'Orleans, Champagne Artique de 1907 1910(Atlas of pls I-XXXVII):1-355
Latitude	48.629353
Longitude	-122.957542
Depth (m)	.5
Collection date	20-AUG-11
Sample collection site	Pacific_Ocean
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
Habitat description	Coastal waters
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	01-OCT-12
Growth medium	F/2 amended seawater
Modifications to growth medium	f/2 amended seawater, replete condition
Temperature (ºC)	20
Salinty (psu)	35
pH	8.3
Light (µmol photons / m2 / sec)	112
Day portion of day:night cycle in hours	24
Nitrate (μmol/L)	882
Ammonium (μmol/L)	100
Phosphate (μmol/L)	38.6
Silicate (μmol/L)	106
Investigation type	Eukaryotes
Other experimental metadata available	12.5um EDTA