Perkinsus marinus ATCC 50439

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Environmental Data
Principle Investigator(s)	Senjie Lin
External sample ID	Permaexp
NCGR Sample ID	MMETSP0922C
Sample accession number	CAM_SMPL_002782
Assembly accession number	CAM_ASM_000600
Combined Assembly Name	Perkinsus-marinus-ATCC50439
Genus	Perkinsus
Species	marinus
Strain	ATCC 50439
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	1032511
18S rRNA	ATGTCTAAGTATAAGCTTTAAACGGCGAAACTGCGAATGGCTCATTAAAACAGTTATAGTTTATTTGGTG ATCGATTACTATTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGTCAAGGCCCGACTTCGGAAGG GCTGCGTTTATTAGATACAGAACCAACCTAGGCTCCGCCTAGTCCTTGTTGGTGATTCATAATAACCCGG CGAATCGCACGGCTTGTCCGGCGATGGACCATTCAAGTTTCTGACCTATCAGCTATGGACGGTAGGGTAT TGGCCTACCGTGGCGTTGACGGGTAACGGGGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG CTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGATACAGGGAGGTAGTGACAAGAA ATAACAATACAGGGCAATTCTGTCTTGTAATTGGAATGAGTAGATTTTAAATCTCTTTACGAGTATCAAT TGGAGGGCAAGTCTGGTGCCAGCAGCCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCG GTTAAAAAGCTCGTAGTTGGATTTCTGCCTTGGGCGACCGATCCACCTTTCCTACGGGATTGGTTGGTAT CAGGTTTGACCTTGGCTTTTTCTTGGGATTCGTGCTCACGTACTTAACTGTGCGTTGACCGTGTTCCAAG ACTTTTACTTTGAGGAAATTAGAGTGTTTCAAGCAGGCTTATGCCATGAATACATTAGCATGGAATAATA GGATATGACTTCGGTCATATTTTGTTGGTTTCTAGGACTGAAGTAATGATTAATAGGGACAGTCGGGGGC ATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGAACTACTGCGAAAGCATTTGCC AAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAA CCATAAACTATGCCGACTAGGGATTGGGGGTCGTTAATTTTAGACGCCCTCAGCACCTCGTGAGAAATCA AAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC CAGGAGTGGAGCCTGCGGCTTAATTTGATTCAACACGGGAAAACTCACCAGGTCCAGACATAGGAAGGAT GACAGATTGATAGTCTTCAGACATAGGAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGG TGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAAC CTGCTAAATAGTTGCGCGAAACTTTATGTTTCGTCGCTACTTCTTAGAGGGACTTTGTGTGTTTAACACA AGGAAGCTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGAC ACGATCAACAAGTATTTCCTTGCCCGGTAGGGTTAGGGTAATCTTTTGAAATCGTGTCGTGCTAGGGATA GACGATTGCAATTATTCGTCTTCAACGAGGAATTCCTAGTAAATGCAAGTCATCAGCTTGCGTTGATTAC GTCCCTG
Importance of organism and transcriptomes	Perkinsus marius is a pathogenic protist causing "dermo" disease among oysters in estuaries, causing dramatic damages to shellfish industries. Members of the genus Perkinsus are closely related to, but distinct from, dinoflagellates. It has two spliced leaders.
Additional citations and references	Dungan and Hamilton 1995, J. Eukaryot. Microbiol. 42:379-388
Primary citation for organism's characterization, if available	Zhang, H., Campbell, D. A, Strum, N. R., Dungan, C. F. and Lin, S. 2011. Spliced leader RNA, mitochondrial gene frameshifts, and multi-protein phylogeny expand support for Perkinsus as a unique alveolate. PLoS ONE 6(5): e19933. Doi:10.1371/journal.pone.0019933.
Latitude	38.71306
Longitude	76.32083
Depth (m)	4
Salinity (psu)	12
Temperature (ºC)	13
Collection date	20-OCT-92
Sample collection site	Chesapeake Bay
Other collection site info	Choptank River, Tilghman Wharf, Maryland
Sample material (e.g. "seawater," "sediment," etc.)	Crassostrea virginica host-oyster
Habitat description	Benthic estuarine oyster reef
Habitat	organism-associated habitat
Country	UNITED STATES
Experimental Data
Date of experiment	13-MAY-11
Growth medium	850 mOsm/kg DME:Ham's F-12 (Burreson et al. 2005, J. Eukaryot. Microbiol. 52:258-270)
Modifications to growth medium	3% (v/v) FBS, 100 ?g/ml streptomycin, 100 U/ml penicillin
Temperature (ºC)	27
Salinty (psu)	29
pH	7
Pressure (atm)	1
Investigation type	Eukaryotes
Other experimental metadata available	submitted is Perkinsus spliced leader based cDNA prepared when culture was in exponential phase.