Perkinsus chesapeaki ATCC PRA-65

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Environmental Data
Principle Investigator(s)	Senjie Lin
External sample ID	Perchexp
NCGR Sample ID	MMETSP0924C
Sample accession number	CAM_SMPL_002745
Assembly accession number	CAM_ASM_000563
Combined Assembly Name	Perkinsus-chesapeaki-ATCC_PRA_65
Genus	Perkinsus
Species	chesapeaki
Strain	ATCC PRA-65
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	321610
18S rRNA	ATTCACACCGATTCATTCTCTGAGAAACCAGCGGTCTCTTCTTCGGAAGAGATGGGATCTCCGCTTTGTT TAGATCCCCACACCTGACCGCTTTAACCGGCTGGGTAGGTGCATAACTTCTATGAACCATATTGTACTAG TCTAAAGTATCCAATATCCTTTTGGATTTTGGTATTTCAAAACGAAATTCCAAACTCTCAACGATGGATG CCTCGGCTCGAGAATCGATGAAGGACGCAGCGAAGTGCGATAAGCACTGCGATTTGCAGAATTCCGTGAA CCAGTAGAAATCTCAACGCATACTGCACAAAGGGGATTTATCCTCTTTGTACATACATATCAGTGTCGCT CTTCTTCCCGATACAAACATTTTGTTGGTTTTTAATCAACTCTATGCTTTGTATCCCGCTTGAATTCCGA TTTATTGGAATTCGCTGTGTGCGCTTGCTAACACAGGCGCATTAATTTGCAAGGCTATAATACTACTTTA AGTAGTACTGTAGCCCCTTCGCAAGAAGGACTGCGCTAGTGAGTATCTTTGGATACTCGCGAACTCGACT GTGTTGTGGTTGATTCCGTGTTCCTTGATCACGTGATTCATCGCTTCAACGCATTATGTCAATCTTGATG AATGCAGAGAAGTTGTTTATGAGTCTCGCGGTCGCTTTGGTCTCAGAATCGTTACTATAACACGCTTGTC GGTTTGCACCATTGGCGATATGTCATCATTAGAAGGCCTGATGTGTGTGAGATT
Importance of organism and transcriptomes	Perkinsus chesapeaki is a pathogenic protist that infects at least 6 species of clams in both Chesapeake Bay and Delaware Bay, at prevalences that commonly reach 100% among clam samples (Reece et al. 2008, Dis. Aquat. Org. 82:237-248). Members of the genus Perkinsus are closely related to, but distinct from, dinoflagellates. It has two spliced leaders.
Additional citations and references	Burreson et al. 2005, J. Eukaryot. Microbiol. 52:258-270.
Primary citation for organism's characterization, if available	Zhang, H., Campbell, D. A, Strum, N. R., Dungan, C. F. and Lin, S. 2011. Spliced leader RNA, mitochondrial gene frameshifts, and multi-protein phylogeny expand support for Perkinsus as a unique alveolate. PLoS ONE 6(5): e19933. Doi:10.1371/journal.pone.0019933.
Latitude	38.9944
Longitude	76.2141
Depth (m)	4
Salinity (psu)	13.5
Temperature (ºC)	27.8
Collection date	27-AUG-02
Sample collection site	Chesapeake Bay
Other collection site info	Chester River, Buoy Rock, Maryland
Sample material (e.g. "seawater," "sediment," etc.)	Mya arenaria host clam
Habitat description	The host clam was infaunal within a benthic sand and mud substrate from which it was excavated.
Habitat	organism-associated habitat
Country	UNITED STATES
Experimental Data
Date of experiment	13-MAY-11
Growth medium	850 mOsm/kg DME:Ham's F-12 (Burreson et al. 2005, J. Eukaryot. Microbiol. 52:258-270)
Modifications to growth medium	3% (v/v) FBS, 100 ?g/ml streptomycin, 100 U/ml penicillin
Temperature (ºC)	27
Salinty (psu)	29
pH	7
Pressure (atm)	1
Investigation type	Eukaryotes
Other experimental metadata available	submitted is Perkinsus spliced leader based cDNA prepared when culture was in exponential phase.