Vaucheria litorea CCMP2940

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Environmental Data
Principle Investigator(s)	Mary Rumpho
External sample ID	Vlit_pooled
NCGR Sample ID	MMETSP0946
Sample accession number	CAM_SMPL_002656
Assembly accession number	CAM_ASM_000474
Combined Assembly Name	Vaucheria-litorea-CCMP2940
Genus	Vaucheria
Species	litorea
Strain	CCMP2940
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	109269
18S rRNA	TGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCAAATCCAAAACAAAAACAAAACATTGTGAACATTTTTTATTCTTGTCGGTGATGCGTGCGTATGTTTTACATACCCGCCTTACCCCCAAGGATATTTTTTCAAAAAAAACCCCTTTTTCACCCCTTCTTTTTTTTTTCTCTCAGAGAAAAAAGAAAAATTAAAAAAGTTTGAAAGTAAAAAAAATATTTTGTTGTTCGCAACAGAATAAATAAAAATACATGACAACTTTTAACGGTGGATCTCTTGGCTCGTGCATCGATGAAGAACGCAGCAAAATGCGATACGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCTCCTTGGCTATCCAGGGAGCATGCCTGTTTGAGTGTCGCCAACATATTCATCCTCAAAGAAAGGAAAAAAAAGTCTAACTTTTTTTCTTCTTGCTCTGTTGTGATGGTGTTGAAGTTCCGAGAAATTCAGTTTTCCTGGTTCTTTTAAAGGTGAGCTGAGAGCAATTTGTTTTCAGCCCTGGTCAAGCGACCCAATCCTTTGACAAGATTGTTTTTACCTTTGTGCCAGCAATGGTGCAATAACTTCTTTTGATGGAAATTCTTTTTCTTTCAACCACAAAAAAAAAATGAAATCGAGTGGTATTTCTCCATTCTTTTTCTTTTTTCCTCTTCCTTTTTTCTTCTTTTCCTCTCACGAGGCAAAAGGGAAAACTGTGATAGTTTAGAGTCTCTGTACTCTGATTTACAAATGAGGATCTTTTCTTTTTGTGTGAACAGGAAAAAAAAAAAGTCAGGAGAAAGGCTAAGATCTGTCAGGTTGTAAAGCACAAAAATATTCGGGCCAGAGCATCCGGTAACACGGGCGCTGCTGACGAAACATTTAACCTGCGACCTCAAATCAGACAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGA
Importance of organism and transcriptomes	The chloroplasts of Vaucheria litorea are used by certain sacoglossan molluscs in kleptoplastic symbiosis. Plastid function and regulation in the alga and the animal is poorly understood. Characterizing the transcriptome of this algal will aid in underst
Additional citations and references
Primary citation for organism's characterization, if available	Agardh, C.A. (1823). Species algarum rite congitae, com synonymis, differentiis specificis et descriptionibus succinctis. Volumen primum pars posterior.pp. [vii-viii],[300]-531. Luncae [Lund]: ex officina Berlingiana.
Depth (m)	.1
Collection date	01-JUL-07
Sample collection site	Atlantic_Ocean
Habitat description	salt marsh
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	30-JUN-11
Growth medium	modified f/2 media
Modifications to growth medium	Final molar concentration of vitamins as follows: Thiamine - HCl =1.184E-07M; Biotin=1.60E-07M; Vitamin B12=2.95E-08M
Temperature (ºC)	18
Salinty (psu)	32
pH	7.5
Nitrate (μmol/L)	.352
Phosphate (μmol/L)	.0144
Total Fe (nmol/L)	5.54
Trace elements (total) (nmol/L)	.4377
Investigation type	Eukaryotes
Other experimental metadata available	RNA will be pooled from algae grown under high, ambient and low light and collected from time points spanning the diurnal cycle.