Marine Microbial Eukaryote Transcriptome Sequencing Project
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Aplanochytrium sp PBS07
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| Principle Investigator(s) | Jackie Collier |
|---|---|
| External sample ID | Aplanochytrium PBS07 horse serum |
| NCGR Sample ID | MMETSP0955 |
| Sample accession number | CAM_SMPL_002681 |
| Assembly accession number | CAM_ASM_000499 |
| Combined Assembly Name | Aplanochytrium-sp-PBS07 |
| Genus | Aplanochytrium |
| Species | sp |
| Strain | PBS07 |
| Clonal | Yes |
| Axenic | Yes |
| Prelim. NCBI Taxon ID | 627963 |
| 18S rRNA | |
| Importance of organism and transcriptomes | The labyrinthulomycetes (including labyrinthulids, oblongichytrids, thraustochytrids, and aplanochytrids) are ubiquitous, diverse, and abundant marine protists (e.g. Collado Mercado et al. 2010 and references therein). They are thought to live mainly as saprobes, obtaining their nutrition from non-living particulate organic matter (POM) of algal, higher plant, or animal origin. Thus, while labyrinthulomycetes are not fungi in a taxonomic sense, they function as fungi in an ecological sense, likely playing important roles in the decomposition of marine POM. It has recently become apparent that the most abundant labyrinthulomycetes in habitats ranging from coastal temperate sediments to the equatorial open ocean water column belong to the genus Aplanochytrium (Collado Mercado et al. 2010, Damare and Raghukumar 2010 and references therein). |
| Additional citations and references | Collado Mercado, E., Radway, J. C. & Collier, J. L. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58:215-28. Damare, V. & Raghukumar, S. 2010. Association of the stramenopilan protists, the aplanochytrids, with zooplankton of the equatorial Indian Ocean. Mar. Ecol. Prog. Ser. 399:53-68. Yokoyama, R. & Honda, D. 2007. Taxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomic characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov. Mycoscience 48:199-211. Leander CA, Porter D (2000) Redefining the genus Aplanochytrium (Phylum Labyrinthulomycota). Mycotaxon 76:439-444. Leander CA, Porter D (2001) The Labyrinthulomycota is comprised of three distinct lineages. Mycologia 93:459-464. |
| Environmental Data | |
| Primary citation for organism's characterization, if available | Leander CA, Porter D, Leander BS (2004) Comparative morphology and molecular phylogeny of aplanochytrids (Labyrinthulomycota). Europ. J. Protistol. 40:317-328. |
| Latitude | 40.93 |
| Longitude | -72.5 |
| Depth (m) | 7 |
| Temperature (ºC) | 13 |
| Collection date | 29-OCT-07 |
| Sample collection site | OTHER |
| Sample material (e.g. "seawater," "sediment," etc.) | sediment (surface 0 to 2 cm) |
| ENVO term for habitat - primary term | Acquatic: marine |
| ENVO term for habitat - secondary term | Other |
| Habitat description | surface sediment in Peconic Bay, Long Island, New York, USA |
| Other environmental metadata available | none available |
| Other environmental metadata available | none available |
| Habitat | marine habitat |
| Country | UNITED STATES |
| Experimental Data | |
| Date of experiment | 26-MAR-12 |
| Growth medium | artificial seawater with 1% horse serum (0.8% agar plates) |
| Modifications to growth medium | artificial seawater (25 grams per liter Instant Ocean) plus 0.8% agar autoclaved, then filter sterilized horse serum (1% vol:vol) added just before pouring plates |
| Temperature (ºC) | 23 |
| Salinty (psu) | 25 |
| pH | 8 |
| Pressure (atm) | 1 |
| Investigation type | Eukaryotes |
| Other experimental metadata available | Harvested 275 mg (wet weight) biomass from 40 plates after 19 to 20 days of growth |