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Aplanochytrium sp PBS07

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Principle Investigator(s) Jackie Collier
External sample IDAplanochytrium PBS07 horse serum
NCGR Sample IDMMETSP0955
Sample accession numberCAM_SMPL_002681
Assembly accession numberCAM_ASM_000499
Combined Assembly NameAplanochytrium-sp-PBS07
GenusAplanochytrium
Speciessp
StrainPBS07
ClonalYes
AxenicYes
Prelim. NCBI Taxon ID627963
18S rRNA
Importance of organism and transcriptomesThe labyrinthulomycetes (including labyrinthulids, oblongichytrids, thraustochytrids, and aplanochytrids) are ubiquitous, diverse, and abundant marine protists (e.g. Collado Mercado et al. 2010 and references therein). They are thought to live mainly as saprobes, obtaining their nutrition from non-living particulate organic matter (POM) of algal, higher plant, or animal origin. Thus, while labyrinthulomycetes are not fungi in a taxonomic sense, they function as fungi in an ecological sense, likely playing important roles in the decomposition of marine POM. It has recently become apparent that the most abundant labyrinthulomycetes in habitats ranging from coastal temperate sediments to the equatorial open ocean water column belong to the genus Aplanochytrium (Collado Mercado et al. 2010, Damare and Raghukumar 2010 and references therein).
Additional citations and referencesCollado Mercado, E., Radway, J. C. & Collier, J. L. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58:215-28. Damare, V. & Raghukumar, S. 2010. Association of the stramenopilan protists, the aplanochytrids, with zooplankton of the equatorial Indian Ocean. Mar. Ecol. Prog. Ser. 399:53-68. Yokoyama, R. & Honda, D. 2007. Taxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomic characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov. Mycoscience 48:199-211. Leander CA, Porter D (2000) Redefining the genus Aplanochytrium (Phylum Labyrinthulomycota). Mycotaxon 76:439-444. Leander CA, Porter D (2001) The Labyrinthulomycota is comprised of three distinct lineages. Mycologia 93:459-464.
Environmental Data
Primary citation for organism's characterization, if availableLeander CA, Porter D, Leander BS (2004) Comparative morphology and molecular phylogeny of aplanochytrids (Labyrinthulomycota). Europ. J. Protistol. 40:317-328.
Latitude40.93
Longitude-72.5
Depth (m)7
Temperature (ºC)13
Collection date29-OCT-07
Sample collection siteOTHER
Sample material (e.g. "seawater," "sediment," etc.)sediment (surface 0 to 2 cm)
ENVO term for habitat - primary termAcquatic: marine
ENVO term for habitat - secondary termOther
Habitat descriptionsurface sediment in Peconic Bay, Long Island, New York, USA
Other environmental metadata availablenone available
Other environmental metadata availablenone available
Habitatmarine habitat
CountryUNITED STATES
Experimental Data
Date of experiment26-MAR-12
Growth mediumartificial seawater with 1% horse serum (0.8% agar plates)
Modifications to growth mediumartificial seawater (25 grams per liter Instant Ocean) plus 0.8% agar autoclaved, then filter sterilized horse serum (1% vol:vol) added just before pouring plates
Temperature (ºC)23
Salinty (psu)25
pH8
Pressure (atm)1
Investigation typeEukaryotes
Other experimental metadata availableHarvested 275 mg (wet weight) biomass from 40 plates after 19 to 20 days of growth