Schizochytrium aggregatum ATCC28209

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Environmental Data
Principle Investigator(s)	Jackie Collier
External sample ID	Schiz. aggregatum ATCC28209 Artemia detritus
NCGR Sample ID	MMETSP0964
Sample accession number	CAM_SMPL_002682
Assembly accession number	CAM_ASM_000500
Combined Assembly Name	Schizochytrium-aggregatum-ATCC28209
Genus	Schizochytrium
Species	aggregatum
Strain	ATCC28209
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	4773
18S rRNA	GAGATaAGCCATGCATGCGATAGTATTAGTTGGTAATACAGCGAAACTGCGAACGGCTCATTATATCTGTTATAATTCCCATGATTGTGCGTTGTGTAGACGGATACATGTGGCAAATCTAGAATCAATACGTG~CTTGGAGG~CCCGA~CTTTTGTGGGA~GGGCCGCACTTATTAGACCTAAACCAGTAGGCTCTCGGGCCTTGTTTCGGTGAGTCATAGTAATTGAGCGAATCGCATAGCCCTCGGGCGGCGATGAATCATTCAAGTTTCAGCCCCATCAGTTGTCGACGGGAGGGTATTGGCCTCCCGTGACCGTTACGGGTGACGGGGAATTAGGGTTCGAGTCCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACTGCAAGTTTACAGAACGAAGTAGTGACGAGAAATAGCAGTGAGGTGCGCTGTGCGTA~TCTCGACTGAAATGAGAGCGATTTAAAACCCCCATCGAGGATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCGAACGTGTGGCGTGCGCGCGGGTCCGTCGGGGGGCGAATGCCCGTCGAGCAGCGAsyCGCGCGsTGCCAGTCTGGCACTtt-tttGAGGTGCCTCTTTCACTGTAAAAAAATTAGGGTGTTTCAAGCAAAGGTTGATATTGGAATATGATAGTATGGGATGATGAGATAGGCCGTTGGCGGTATTTTGTTGGTTTGCGCGTTGGCGGAATGATGAAGAGGGACAGTTGGGGGTATTCGAATTCTAGTGTCAGAGGTGAAATTCTTGGATTACTAGAAGGCGAACGACTGCGAAAGCATTTACCAAGGATGTTTTCATTGATCAAGAACGAAAGTATGGGGATCGAAGATGATTAGATACCATCGTAGTCCATACCGTAAACGATGCCGACTCGCGATGGTCCCCTgGCTCTAGTGGCGGGGGGCGGCAGCGCATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGGAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTGATTCCGTTAACGAACGAGACCACAGCCTrCTAAATAGTGCGCGTTGTGGCGACACAGTGAGrGGCACTTCTTAGAGGGACACTTTGGGTTTACCAGAGGGAAGTTTGTGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGACTGGGGGAGCACGTksykkkGkCGTmyGwCmwyGTCCTGGGCCGGAAGGTTTGGGyAATCGTGTGAATACCAGTCGTGATGGGGCTAGATCTTTGCAATTATTGATCTCCAACGAGGAATTCCTAGTAAACGCAAGTCATCAGCTTGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAACGTTCCGGTGAGACCTTCGGAGGGCACGATGTTCCTGGCGACAGGACGTGGTGGCCAAAGTTGAGCAAACCTTAACGTTtAGAgGAAGG
Importance of organism and transcriptomes	The labyrinthulomycetes (including labyrinthulids, oblongichytrids, thraustochytrids, and aplanochytrids) are ubiquitous, diverse, and abundant marine protists (e.g. Collado Mercado et al. 2010 and references therein). They are thought to live mainly as saprobes, obtaining their nutrition from non-living particulate organic matter (POM) of algal, higher plant, or animal origin. Thus, while labyrinthulomycetes are not fungi in a taxonomic sense, they function as fungi in an ecological sense, likely playing important roles in the decomposition of marine POM. Among the labyrinthulomycetes, Schizochytrium aggregatum ATCC28209 was among the first described in detail, is thought to be cosmopolitan, and is probably the best studied. Schizochytrium aggregatum has specifically been shown to degrade cellulose (Bremer and Talbot 1995) and so may be particularly well-suited to growth on refractory material of terrestrial origin, and provide a conduit for this material into marine food webs.
Additional citations and references	Collado Mercado, E., Radway, J. C. & Collier, J. L. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58:215-28. Bremer, G. B. & Talbot, G. 1995. Cellulolytic enzyme activity in the marine protist Schizochytrium aggregatum. Botanica Marina 38:37-41.
Primary citation for organism's characterization, if available	Goldstein, S. & Belsky, M. 1964. Axenic culture studies of a new marine phycomycete possessing an unusual type of asexual reproduction. American Journal of Botany 51:72-78
Latitude	8
Longitude	53
Collection date	15-JUL-63
Sample collection site	North Sea
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Other
Habitat description	Seawater off West Germany
Other environmental metadata available	none available
Other environmental metadata available	none available
Habitat	marine habitat
Country	GERMANY
Experimental Data
Date of experiment	11-JUN-12
Growth medium	artificial seawater with 0.125% Artemia detritus (0.8% agar plates)
Modifications to growth medium	artificial seawater (25 grams per liter Instant Ocean), 0.125% freeze dried and ground Artemia (wt:vol), solidified with 0.8% agar
Temperature (ºC)	23
Salinty (psu)	25
pH	8
Pressure (atm)	1
Investigation type	Eukaryotes
Other experimental metadata available	Harvested 491 mg (wet weight) biomass from 10 plates after 10 days of growth