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Emiliania huxleyi 374


Principle Investigator(s) Kay Bidle
External sample IDEhux374C24
Sample accession numberCAM_SMPL_002667
Assembly accession numberCAM_ASM_000485
Combined Assembly NameEmiliania-huxleyi-374
Prelim. NCBI Taxon ID2903
18S rRNA
Importance of organism and transcriptomesThe coccolithophore Emiliania huxleyi forms massive annual spring blooms in the North Atlantic that are routinely infected and terminated by lytic, double-stranded DNA containing Coccolithoviruses. Given the wide array of sensitive and resistant host strains in culture, along with a number of genetically diverse E. huxleyi-infecting viruses (EhVs), the E. huxleyi-EhV host-virus system has emerged as one of the best model systems to investigate host-virus interactions and the cellular processes mediating infection dynamics. However, little is known about the molecular mechanisms mediating phytoplankton host-virus interactions. This project aims to sequence transcriptomes for both uninfected (control) and EhV86-infected E. huxleyi strains that span a range of viral susceptibility in order to elucidate the comparative global, subcellular transcriptional response associated with either resistance or sensitivity to infection. Transcriptome datasets will consist of control and EhV86-infected cells for the hyper-resistant strain CCMP379 (Ehux379) and the hypersensitive strain CCMP374 (Ehux374) at 2 h and 24 h of lytic infection. This information will shed novel insight into the subcellular mechanisms of resistance and sensitivity, thereby allowing us to better elucidate the evolutionary relationship between E. huxleyi and its viruses. It may also reveal unprecedented molecular insight into a possible innate immune response to protect against infection and ultimately influence which strains escape viral demise. Aside from a few isolated genes in GenBank [putative calcium binding protein (gpa), cytochrome oxidase (coi) and actin], no transcritomic information is currently available for Ehux379 or Ehux374 strains. Hence, these transcriptomic datasets will not only provide novel inter-strain comparisons for global gene expression response to virus challenge, but they will also provide data for an extensive dataset for comparison with the available ESTs (which include EhV-infected cells) and the reference genome information for E. huxleyi CCMP1516 (http://genome.jgi-psf.org/Emihu1/Emihu1.home.html), which is also sensitive to EhV infection.
Additional citations and references
Environmental Data
Depth (m)5
Collection date23-JUN-89
Sample collection siteOTHER
Other collection site infoGulf of Maine
Sample material (e.g. "seawater," "sediment," etc.)seawater
ENVO term for habitat - primary termAcquatic: marine
Habitatmarine habitat
Experimental Data
Date of experiment01-AUG-11
Growth mediumf/2 - Si amended seawater
Modifications to growth mediumf/2 for all nutrients except silica, which was not added
Temperature (ºC)18
Salinty (psu)20
Light (µmol photons / m2 / sec)200
Day portion of day:night cycle in hours14
Night portion of day:night cycle in hours10
Nitrate (μmol/L)883
Phosphate (μmol/L)36.2
Trace elements (total) (nmol/L)1200
Investigation typeEukaryotes