Karlodinium micrum CCMP2283

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Environmental Data
Principle Investigator(s)	Geoff Sinclair, Boris Wawrik
External sample ID	K.micrum L1 - P
NCGR Sample ID	MMETSP1017
Sample accession number	CAM_SMPL_002694
Assembly accession number	CAM_ASM_000512
Combined Assembly Name	Karlodinium-micrum-CCMP2283
Genus	Karlodinium
Species	micrum
Strain	CCMP2283
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	342587
18S rRNA	ATAGGTTCACTAAACTTTCCGCTGCAACGTTCAGGAACTAAACACTGCAGCAGTCCGAATTATTCACCGGATCACTCAAT CGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAGCACGAGCTGATGACTCGCGCTTACTAGGAATTC CTCGTTGAAGATTAATAATTGCAATAATCTATCCCCATCACGATGCGTTTTAAAAAGATTACCCAATCTTTCCAGACAAG GTCATAAACTCGTTGAGCGCATCAGTGTAGCGCGCGTGCAGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCT CAAACTTCCTTGCGTTAGACACGCAAAGTCCCTCTAAGAAGTTGCCCACGTAACAGAAGTTACGTGTAACTATTTAGCAG GTTAAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCACTCCACCAACTAARAACGGCCATGCACCACCACCCATAG AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTATGTCTGGACCTGGTAAGTTTCCCCGTGTTGAGTCAAATTAAGC CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA AAGACTTTGATTTCTCATAAGGTGCTGAAGGAGTCGTATGAGTAACGACCTCCAATCTCTAGTCGGCATGGTTTATGGTT AAGACTAGGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTT CGCAGTAGTCCGTCTTTAACAAATCCAAGAATTTCACCTCTGACAGTTAAATACGAATGCCCCCAACTATCCCTATTAAT CATTACCTCAGCTCTAGAAACCAACAAAATAGAACCGAGGTCCTATCTTATTATTCCATGCTAATGTATTCAAGGCGTGT GCCTGCTTGAAACACTCTAATTTCCTCAAAGTAAAAGTCCTGGATACCGCACCACACAGTCAAGTGCAGATACGTTCTCC AAGAAGATGCCCAGGCCGAGCCAGATACGCACCCAGAGGGCGGACCGGTCGTCCTCGGCAGAAATCCAACTACGAGCTTT WTAACCGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGAT ACTCGAAAAGGGATTTAAATTCTACTCATTCCAATTACAAGACATGGATGCCCTGTATTGTTATTTCTTGTCACTACCTC CCTGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA TCGAACCCTAATTCTCCGTTACCCGTCATTGCCACGGTAGGCCAATACCCTACCGTCGGAAGCTGATAGGTCAGAAACTT GAATGATTTATCGCCAGCGGAAGCTATGCGATTCGTTCGGTTACTATGAATCACCACAATACCAGGGCGAACCTGGGTTG GTTCTGTATCTAATAAACACAACCCTTCCACGAAGTCGGGTTTGGGCGCATGTATTAGCTCTAGAATTACCACAGTTATC CATGTAATGAATGACCATCAAATAAACTATAACTGTTTTAATGAGCCATTCGCAGTTTCGCCGTATAGAAGCTTATACTG AGACATGCA
Importance of organism and transcriptomes	Dinoflagellates are a key component of the eukaryotic phytoplankton in all of the world?s oceans. Despite their importance, mechanistic understanding of their physiological responses to important environmental changes, such as nutrient loading remains relatively poorly understood, since very little genetic information is available due to the immense size of their genomes. Transcriptome sequencing will provide significant insight into the genomic complexity of this group and a foundation upon which to explore the linkages between genomic and phenotypic responses in response to environmental changes that threaten coastal ecosystems.
Additional citations and references
Latitude	32.2167
Longitude	80.7355
Habitat description	coastal
Other environmental metadata available	Isolate from tidally influenced retention pond, Marshide Properties, Hilton Head, South Carolina USA
Other environmental metadata available	Isolate from tidally influenced retention pond, Marshide Properties, Hilton Head, South Carolina USA
Habitat	marine habitat
Experimental Data
Date of experiment	01-JUL-07
Growth medium	L1
Modifications to growth medium	none
Temperature (ºC)	22
Salinty (psu)	20
pH	8.2
Light (µmol photons / m2 / sec)	60
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	60
Trace elements (total) (nmol/L)	1170
Prey organism, if applicable (genus and species)	none