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Lingulodinium polyedra CCMP 1738

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Principle Investigator(s) Mary Ann Moran
External sample ID1738 dark 8 hrs
NCGR Sample IDMMETSP1033
Sample accession numberCAM_SMPL_002587
Assembly accession numberCAM_ASM_000405
Combined Assembly NameLingulodinium-polyedra-CCMP1738
GenusLingulodinium
Speciespolyedra
StrainCCMP 1738
ClonalYes
AxenicNo
Prelim. NCBI Taxon ID160621
18S rRNA
Importance of organism and transcriptomesDinoflagellates and bacteria are intimately associated in the marine environment, and many bacteria can break down phytoplankton-derived dimethylsulfonium compounds, like DMSP. L. polyedra also produces some of the lesser known dimethylsulfonium derivatives, including gonyauline and/or gonyol. We are interested in how the production of these compounds as well as the interaction between dinoflagellates and bacteria vary under different conditions (i.e. light versus dark). This work will also provide insight into multi-derivative-dimethylsulfonium-producing dinoflagellates (i.e., those that produce sulfonium compounds other than DMSP) and the fate of these compounds in the oceans and atmosphere.
Additional citations and referencesTanikawa, N., H. Akimoto, K. Ogoh, W. Chun, and Y. Ohmiya. 2004. Expressed sequence tag analysis of the dinoflagellate Lingulodinium polyedrum during dark phase. Photochem Photobiol 80:31-5. Mayali, X., P. J. Franks, and F. Azam. 2008. Cultivation and ecosystem role of a marine Roseobacter clade-affiliated cluster bacterium. Appl. Environ. Microbiol. 74:2595-603
Environmental Data
Latitude27.8
Longitude97.13
Sample collection siteGulf of Mexico
Other collection site infoTexas Coast
ENVO term for habitat - primary termAcquatic: marine
ENVO term for habitat - secondary termNot Applicable
Habitat descriptionGulf of Mexico off the coast of Texas
Habitatmarine habitat
CountryUNITED STATES
Experimental Data
Date of experiment09-SEP-11
Growth mediumf/2 - Si artificial seawater
Modifications to growth mediumf/2 for all nutrients, minus the addition of Si
Temperature (ºC)18
Salinty (psu)28
pH8.3
Light (µmol photons / m2 / sec)150
Day portion of day:night cycle in hours12
Night portion of day:night cycle in hours12
Nitrate (μmol/L)882
Phosphate (μmol/L)36.2
Trace elements (total) (nmol/L)24500
Investigation typeEukaryotes
Other experimental metadata availableChlorophyll, DMSPt, DMSPd, and DMSPp will be measured. Dimethylsulfonium derivatives will also be analyzed.