Azadinium spinosum 3D9

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Environmental Data
Principle Investigator(s)	Uwe John
External sample ID	3D9_C
NCGR Sample ID	MMETSP1036_2
Sample accession number	CAM_SMPL_002590
Assembly accession number	CAM_ASM_000408
Combined Assembly Name	Azadinium-spinosum-3D9
Genus	Azadinium
Species	spinosum
Strain	3D9
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	632150
18S rRNA	CCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTCAGTATAAGCTTCTATACGGCGAAACTGCGAATGGCTCATTAAAACAGTTATAGTTTATTTGATGGTCATTTCTTACATGGATAACCGTGGTAATTCTAGAGCTAATACATGCGCCCATACCCGACTCTGTGGAAGGGTTGTGTTTATTAGTTACAGAACCAACCCAGGCTCTGCCTGGTCTTGTGGTGATTCATAATAACCGAACGAATCGCATGGCATCTGCTGGCGATGAATCATTCAAGTTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCCTACCGTGGCAATGACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAGTAGAATTTAAATCCCTTTGCGAGTATCGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGCCGAGGACGACCGGTCCGCCCTCTGGGTGAGTATCTGGCTCGGCCTGGGCATCTTCTTGGAGAACGTAGCTGCACTTGACTGTGTGGTGCGGTATCCAGGACATTTACTTTGAGGAAATTAGAGTGTTTCAAGCAGGCATACGCCTTGAATACATTAGCATGGAATAATAAGATAGGACCTCGGTTCTATTTTGTTGGTTTCTAGAGCTGAGGTAATGATTAATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACCATGCCGACTAGAGATTGGAGGTCGTTATCTATACGACTCCTTCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACACCTAACCTCGGTTAGGTGGGCAACTTCTTAGAGGGACTTTGCGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCTGCACGCGCGCTACACTGATGCGCTCAACGAGTTTATGACCTTGTCCGGCAGGATTGGGTAAT
Importance of organism and transcriptomes	Azadinium spinosum is the source organism of azaspiracid. The ordinal affiliation of the genus is still under debate and might represent in new group within the dinoflagellates. The size group of organisms of 3-20?m is relative under represented in diversity, taxonomic and genomic studies.
Additional citations and references
Primary citation for organism's characterization, if available	Tillmann,U., Elbraechter,M., Krock,B., John,U. and Cembella,A.(2009). Azadinium spinosum gen. et sp. nov. (Dinophyceae) identified as a primary producer of azaspiracid toxins. Eur. J. Phycol. 44 (1), 63-79
Latitude	57.0525
Longitude	2.50056
Salinity (psu)	34
Temperature (ºC)	12
Collection date	15-JUN-07
Sample collection site	North Sea
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
Filter fraction minimum (μm)	3
Filter fraction maximum (μm)	20
Volume filtered (L)	1
Habitat description	coastal North Sea water
Habitat	marine habitat
Country	UNITED KINGDOM
Experimental Data
Date of experiment	15-MAY-11
Growth medium	half stength K (Keller et al. 1987) amended seawater
Modifications to growth medium	supplemented with selenite (Dahl et al. 1989)
Temperature (ºC)	20
Salinty (psu)	32
pH	8
Light (µmol photons / m2 / sec)	90
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	88
Phosphate (μmol/L)	3.6
Total Fe (nmol/L)	1.17
Trace elements (total) (nmol/L)	107.6