Skeletonema marinoi FE60

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Environmental Data
Principle Investigator(s)	Giovanna Romano
External sample ID	FE60
NCGR Sample ID	MMETSP1040
Sample accession number	CAM_SMPL_002594
Assembly accession number	CAM_ASM_000412
Combined Assembly Name	N/A
Genus	Skeletonema
Species	marinoi
Strain	FE60
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	267567
18S rRNA	AACCTGGTTGATCCTGCCAGTAGTCATACGCTCGTCTCAAAGATTAAGCCATGCATGTGTAAGTATAAGATACTTTATCGAAACTGCGGACGGCTCATTATATCAGTTATAGTTTATTTGATAATCCCTCACTACTTGGATAACCGTAGTAATTCTAGAGCTAATATATGCATCAAAGCGGAACTCTCGGGAACCGCCGTGTTTATTAGTATTAAACCTTCACTCTTCGGAGTTGATTTGGTGAGTCATAATAACCTTTCGAATTGCATGACCACGTGTCGGCAATGGATCATTCAAGTTTCTGCCCTATCAGCTTGGGATGGTAGTGTATTGGACTACCATGGCTTTAACGGGTAACGAATTGTTAGGGCAAGATTTCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATGCCGGGCCTTTACAGGTCTGGCAATTGGAATGAGAACAATTTAAATCCCTTATCGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGCAGGAGTGACTGACCACAAACTCTGTTTGTGAGTTGTGTCATTCTGGCCATCCTTGGTAAGATCCTTTTTGGCATTAAGTTGTCGGGAAGGGGATGACCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCTTATGCCGTTGAATATATTAGCATGGAATAATAAGATAGGACTTTGAGTCTATTTTGTTGGTTTGCGAGTCAAAGTAATGATTAATAGGGACAGTTGGGGGTATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTCTGAAAGACGAACTACTGCGAAAGCATTTACCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTCAGGATTGGCGGTTGTTTTTTGACTCCGTCAGCACTGTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGAAATTGACGGAAGGGCACCACCAGGAGTGGAACCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGTTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGCCGCCTGCTAAATAGACCCGCGAATGGCTTTTCATTGGCGAGGTCTTCTTAGAGGGACGTTCATTCTACAAGATGAAGGAAGATGGCGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCCGCACGCGCGTTACACTGATGCACTCAACGAGTATATAACCTTGGCCGAGAGGCCTGGGTAATCTTGTTAACTTGCATCGTGATAGGGATAGATTATTGCAATTATTAATCTTGAACGAGGAATTCCTAGTAATCGCAGTTCATCAAACTGCAATGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAGACTCGAGATNTTGGTCTAGCTCCTTTATTGGGGCTTGACCGCGAGAACCTGTCTAAACCTTATCATTTAGAGGAAGGTGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATCAA
Importance of organism and transcriptomes	Skeletonema marinoi is a successful centric planktonic marine diatom responsible for spring blooms in North Adriatic sea and in many coastal areas around the world. It has been shown that diatoms produce a class of compounds, the oxylipins, that have been found to negatively impact copepod reproduction with possible negative consequence on the flow of energy through the marin food chain. Definition of the transcriptome of two clones of Skeletonema marinoi showing different levels in oxylipin production, will help to understand the physiological mechanisms underlying the activation and the modulation of oxylipins methabolism in diatoms.
Additional citations and references
Primary citation for organism's characterization, if available	Sarno, D., Kooistra, W. H. C. F., Medlin, L. K., Percopo, I. and Zingone, A. (2005), DIVERSITY IN THE GENUS SKELETONEMA (BACILLARIOPHYCEAE). II. AN ASSESSMENT OF THE TAXONOMY OF S. COSTATUM-LIKE SPECIES WITH THE DESCRIPTION OF FOUR NEW SPECIES. Journal of Phycology, 41: 151?176. doi: 10.1111/j.1529-8817.2005.04067.x
Latitude	43.5
Longitude	14.5
Depth (m)	2
Salinity (psu)	37.0423
Temperature (ºC)	18
Collection date	25-FEB-05
Sample collection site	Adriatic Sea
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
Habitat description	coastal waters, surface layer
Habitat	marine habitat
Country	ITALY
Experimental Data
Date of experiment	15-SEP-11
Growth medium	f/2 amended sea water
Temperature (ºC)	20
Salinty (psu)	36
pH	8.2
Light (µmol photons / m2 / sec)	100
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	883
Phosphate (μmol/L)	36.3
Silicate (μmol/L)	107
Trace elements (total) (nmol/L)	24500
Investigation type	Eukaryotes