Bigelowiella natans CCMP623

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	Bnat623.1
NCGR Sample ID	MMETSP1052
Sample accession number	CAM_SMPL_002602
Assembly accession number	CAM_ASM_000420
Combined Assembly Name	N/A
Genus	Bigelowiella
Species	natans
Strain	CCMP623
Clonal	Unknown
Axenic	Unknown
Prelim. NCBI Taxon ID	227086
18S rRNA	TGTCTAAGTATAAACAACTTTGTACTGTGAAACTGCAGATGGCTCATTAAATCAGAAATAATTTCTTTGA TGGTGAAAATCTACACGGATACCCGTAGTAATTCTAGAGCTAATACGTGCGTAAACTCCATTGGAGGTAT TTATTAGATCCGAACCAGCCCCTTCGGGGTTTTCTGGTGAATCATGATAACTGTTCGAATCGCCAGGCTC TGCCAGGCGATAGTTCATTCAAATTTCTGCCCTATCAGCTATTGACGGTAGTGTAGTGGACTACCGTGGC CGTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG GAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTAGTGACAAGAAATATCGTGACTGTG CCCTTTGTGGCAGAGTCTTCGAAATGAGCAATACGTAAATGCCTTTGCGAGTATCCATTGGAGGGCAAGT CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCG TAGTTGGATATCTGGCTTTGCACGGACGGCCCGCCTTCGGGTTGAGTGCTGGAAGTGCTAGCCTATTTTC TGGTTGGCGTTCTTTCCCTTAATTGTGGCGAGTTTCGCGATCCGGATCGTTTACTTTGAAGAAATTCGAG TGTTCAAAGCAGGCTTACGCCGTGAAGATTGCAGCATGGAATAATGACATAGGACTATCGTGTTCTATTT TGTTGGTTCTGGGACCGTAGTAATGATTGATAGGGACAGTTGGGGGTGCTGCTATTCCGCGGCCAGAGGT GAAATTCTCAGATTCCCGGAAGAGCAACTTATGCGAAAGCATTCACCAAGGATGTCTTCTTTAATCAAGA ACGAAAGTAGGGGGATCGAAGACGATCAGATACCGTCGTAGTCTCTACCATAAACTATGCCGACTAGGGA TTGGTGGACGTTGTTTTATCCGACTCCATCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGG AGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGACACCACCAGAAGTGGAACTTGCGGCTT AATTTGACTCAACACGGGAAAACTTTCCAGGTCCAGACATAGTAAGGATTGACAGATTGAAGATCTTTCT TGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAAC GAACGAGACCTCGACCTGCTAAATAGGTTCAGTATCGATGCGTCGATGCTTGGAGCTTCTTAGAGGGACT ATCGGATCCAAACCGGTGGAAGTGCGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCA CGCGTGTTACACTGACGCATTCATCAAGCGCCACACCTTGGCTCGAGAGGCCTCGGTAATCTTGAAAGTG CGTCGTGTTAGGGATAGATTATTGTAATTATTAGTCTTGAACGAGGAATTTCTAGTAAACGCGAGTCATC AACTCGCATTGATTACGTCCCTGTCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATCATTTGGT GAGGCTCACGGACTGTCGTGCTTCCTTCCTCGTGTTGGTTGTACTTCTTGGGAA
Importance of organism and transcriptomes	CCMP strain 623 is a chlorarachniophyte alga closely related to Bigelowiella natans. Chlorarachniophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between chlorarachniophytes and other red and green algal secondary plastid-bearing organisms will also be possible.
Additional citations and references
Experimental Data
Date of experiment	01-DEC-11
Growth medium	K
Modifications to growth medium	K without silicate
Temperature (ºC)	23
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Ammonium (μmol/L)	50
Phosphate (μmol/L)	10
Trace elements (total) (nmol/L)	122766