Bigelowiella natans CCMP1258.1

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	CCMP1258.1
NCGR Sample ID	MMETSP1055
Sample accession number	CAM_SMPL_002604
Assembly accession number	CAM_ASM_000422
Combined Assembly Name	N/A
Genus	Bigelowiella
Species	natans
Strain	CCMP1258.1
Clonal	Unknown
Axenic	Unknown
Prelim. NCBI Taxon ID	227086
18S rRNA	GCATGTCTAAGTACAAACAACTTTGTACTGTGAAACTGCAGATGGCTCATTAAATCAGAAATAATTTCTT TGATGGTGAAAATCTACACGGATACCCGTAGTAATTCTAGAGCTAATACGTGCGTAAACTCCATTGGAGG TATTTATTAGATCCGAACCAGCCCCTTCGGGGTTTTCTGGTGAATCATGATAACTGTTCGAATCGCCAGG CTCTGCCAGGCGATAGTTCATTCAAATTTCTGCCCTATCAGCTATTGACGGTAGTGTAGTGGACTACCGT GGCCGTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC AAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACACGGGGAGGTAGTGACAAGAAATATCGTGACT GTGCCCTTTGTGGCAGAGTCTTCCGAAATGAGCAATACGTAAATGCCTTTGCGAGTATCCATTGGAAGGC AAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAGC TCGTAGTTGGATATCTGGCTTTGCACGGACGGCCCGCCTTCGGGTTGAGTGCTGGAAGTGCTAGCCTATT TTCTGGTTGGCGTTCTTTCCCTTAATTGTGGCGAGTTTCGCGATCCGGATCGTTTACTTTGAAGAAATTC GAGTGTTCAAAGCAGGCTTACGCCGTGAAGATTGCAGCATGGAATAATGACATAGGACTATCGTGTTCTA TTTTGTTGGTTCTGGGACCGTAGTAATGATTGATAGGGACAGTTGGGGGTGCTGCTATTCCGCGGCCAGA GGTGAAATTCTCAGATTCCCGGAAGAGCAACTTATGCGAAAGCATTCACCAAGGATGTCTTCTTTAATCA AGAACGAAAGTAGGGGGATCGAAGACGATCAGATACCGTCGTAGTCTCTACCATAAACTATGCCGACTAG GGATTGGTGGACGTTGTTTTATCCGACTCCATCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGG GGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGACACCACCAGAAGTGGAACTTGCGG CTTAATTTGACTCAACACGGGAAAACTTTCCAGGTCCAGACATAGTAAGGATTGACAGATTGAAGATCTT TCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTT AACGAACGAGACCTCGACCTGCTAAATAGGTTCAGTATCGATGCGTCGATGCTTGGAGCTTCTTAGAGGG ACTATCGGATCCAAACCGGTGGAAGTGCGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCC GCACGCGTGTTACACTGACGCATTCATCAAGCGCCACACCTTGGCTCGAGAGGCCTCGGTAATCTTGAAA GTGCGTCGTGTTAGGGATAGATTATTGTAATTATTAGTCTTGAACGAGGAATTTCTAGTAAACGCGAGTC ATCAACTCGCATTGATTACGTCCCTGTCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATCATTT GGTGAGGCTCACGGACTGTCGTGCTTCCTTCCTCGTGTTGGTTGTACTTCTTGGGAAGTGATACAACCTG ATGA
Importance of organism and transcriptomes	CCMP strain 1258 is a chlorarachniophyte alga closely related to Bigelowiella natans. Chlorarachniophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between chlorarachniophytes and other red and green algal secondary plastid-bearing organisms will also be possible.
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Experimental Data
Date of experiment	01-DEC-11
Growth medium	K
Modifications to growth medium	K without silicate
Temperature (ºC)	23
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Ammonium (μmol/L)	50
Phosphate (μmol/L)	10
Trace elements (total) (nmol/L)	122766