Ceratium fusus PA161109

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Environmental Data
Principle Investigator(s)	Susanne Menden-Deuer
External sample ID	C. fusus - LL
NCGR Sample ID	MMETSP1075
Sample accession number	CAM_SMPL_002610
Assembly accession number	CAM_ASM_000428
Combined Assembly Name	Ceratium-fusus-PA161109
Genus	Ceratium
Species	fusus
Strain	PA161109
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	2916
18S rRNA	CTAGTGATAAGGTTCACTAAACTTCCCACNGCAATGTCAGAAGCTGAGCAATGCAGCAGTCCAAATTATTCACCGGATC ACTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAA TCAACATGAGCTGATGACTCACGCTTACTAGGAATTCCTCGTTGAAGATT AATAATTGCAATAATCTATCCCCATCACGATGCGCTTTGACAAGATTACC AAACCTTTCCAGGACAGGTTAAAGACTCATTGACCGCATCAGTGTAGCGC GCGTGCAGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAA ACTTCCTTGCATTAAACACACAAAGTCCCTCTAAGAAGTTGCCCACATAA CAGAAGTTGAGTGTAACTATTTAGCAGGTTAAGGTCTCGTTCGTTAACGG AATTAACCAGACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCA CCCATAGAATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTATGTCTG GACCTGGTAAGTTTCCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACT CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCAT ACTCCCCCCGGAACCCAAAGACTTTGATTTATCATAAGGTGCTGAAGGAA TCATGCACATAACAATCTCCAATCTCTAGTTGGCATGGTTTATGGTTAAGACTAGGA CGGTATCTAATCGTCTTCGATCCCCTAACTTTCGTTCTTGATCAATGAAA GCATCCTTGGCAAATGCTTTCGCAGTCGTTCATCCTTAACAAATCCAAGA ATTTCACNTCTGACAGTTAAATATGAATGCCCCCAACTATCCCTATTAAC CATTACTCTAATTCTAGAAGCCAACAAAATAAAACCACAGTCATATCATA TTATTCCATGCTAGTGTATTCAAGGCAATTGCCTGCTTGAAACACTCTGA TTTCCTCAAAGTAAATGGCTTAGCCTTCACACCACACAGTCAAGTGCAGC TGCAAAGTCTAAGCATATGCCAAAATAAAGCCAGATGCTCACCCAGAGGG CGGACCGGTTTGCTTCAGCAGAAATCCAACTACGAGCTTTTTAACCGCAA CAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACC AGACTTGCCCTCCAATTGATACTCACAGAGAAAGTTAACATCTGTCCATT CCAATTACAAAACATGGATGCCCTGTATTGTTATTTCTTGTCACTACCTC CCTATGCCAAGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTAGATGTG GTAGCCATTTCTCAGGCTCCCTCTCCGGAATCAAACCCTAATTCTCCGTC ACCCGTCACTGCCACGGTAAGCCCATACTTTACCGTCGGAAGCTGATAGG TCAGAAACTAAAACAATTCATTGCCAGCATGCGCCACGCAATTCATTAAT TTATCATCAGTCATACAAAACATCAAGCAGAGCTTGAGTTGGTTTTGAAG TCAATAAACACAACCATACTTGAAGGTCAGGTTTGTGTGCATGTATTAGC TCTAGAATTACCACAGTTATCCATGTAAGCTAAGGTTATTGAGCATATTA TAACTGATATAATGAGCCATTCGCAGTTTCACCAAAAATTAGCTTAAACT CAGACATGCA
Importance of organism and transcriptomes	The genus Ceratium represents a prominent mixotrophic dinoflagellate group. The transcriptome will provide information on gene expression as a function of light level, and possibly help identify genes indicative of hetero- vs. phototrophy. Ultimately, we aim to use these data to quantify mixotrophy in situ.
Additional citations and references	doi:10.3354/ame017167; doi:10.1016/j.hal.2008.02.007
Primary citation for organism's characterization, if available	doi:10.1111/j.1550-7408.2002.tb00343.x
Latitude	41.57083
Longitude	-71.40517
Depth (m)	5
Salinity (psu)	27.87
Temperature (ºC)	11.3
Collection date	16-NOV-10
Collection time	07:00 AM
Sample collection site	OTHER
Other collection site info	Long term plankton time series station, Narragansett Bay
Sample material (e.g. "seawater," "sediment," etc.)	seawater
ENVO term for habitat - primary term	Acquatic: marine
Habitat description	Estuary
Other environmental metadata available	www.gso.uri.edu/phytoplankton
Other environmental metadata available	www.gso.uri.edu/phytoplankton
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	17-NOV-11
Growth medium	f/2-Si amended seawater
Modifications to growth medium	f/2 for all nutrients except eliminate Si
Temperature (ºC)	15
Salinty (psu)	29.4
Light (µmol photons / m2 / sec)	20
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	10
Investigation type	Eukaryotes
Other experimental metadata available	not axenic; grown at 120uE/m2/s until near peak exponential and moved to 20uE/m2/s for three days