Cyanoptyche gloeocystis SAG4.97

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Environmental Data
Principle Investigator(s)	Alexandra Z. Worden
External sample ID	CyaGle4.97
NCGR Sample ID	MMETSP1086
Sample accession number	CAM_SMPL_002619
Assembly accession number	CAM_ASM_000437
Combined Assembly Name	N/A
Genus	Cyanoptyche
Species	gloeocystis
Strain	SAG4.97
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	77921
18S rRNA	GAGAATGGCTCATTAAATCAGTTATAGTTTATTTGATGGTACTCCCTACTTGGATAACCGTAGATAATTCTAGAGCTAAT ACATGCTTAAAATCCCAACTTCTGGAAGGGATGTATTTATTACATAAAAAACCAATGTGTTGGGTTCACCCCCGACAGCC CCTTGGTGATTCATAGTAACTCCTCGGATCGCATGGCTTCACGCCAGTGACGCTTCATTCAAATTTCTGCCCTATCAACT TTCGATGGTAGGATAGAGGCCTACCATGGTGTTAACGGGTAACGGAGAATTAGGGTTTGATTCCGGAGAGGGCGCCTGAG AGATGGCGACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCGCAACTCGCGGAGGTAGTGACNATAAATAA CAATATGGGGCTTCAATAGTCTCGTAATTGGAATGAGAACAATTTAAATCCCTTATCGAGGATCAATTGGAGGGAAAGTC TGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTT CNGAGTAGGGCATTTGGTCCGCCTTATGGTGTGCACTTCTTGTCCCTTCTCTTCTTTCTGAGGACAGTCACTGCCCTTAA CTGAGTGGTGGCTGGACACAGATCCTTTACTTTGAGTAAATTGGAGTGTTTAAAGCAGGCATATGCTCTGAACATTATTA GCATGGAATAATAAAATAAGACTCTGGNTCTTATTTTGTGGTTTCAAGGCCCAGGGTAATGATTAACAGGGATAGTTGGG GCCATTCGTATTCCGTTGTCAGAGGTGAAATTCTTGGATTTACGGAAGACGAACTACTGCGAAAGCATTTGCCAAGGATG TTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTCTTAACCATAAACAATGCCGGC TAGCAATTATTGGGTGGTATTATAACGCCCCCAGTGGAGGCTTATGAGAAATCAAAGCGTTTGGGTTCCGGGGGGAGTAT GGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACAC GGGGAAACTTACCAGGTCCGGACATAGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTACTAAATAGTTACGCGA ACTTTTCGTTCGTGGTCAACTTCTTAGAGGGACTATCTGTGTCTAACTGATGGAAGTTTGAGGCAATAACAGGTCTGTGA TGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGAATTCAACGAGTTCTCCCTTGACCGAAAGGTCTGGGTAA TCTTTTGAAACTTCATCGTGATGGGGATAGATCATTGCAATTATTGATCTTCAACGAGGAATTCCTAGTAAGCGTGAGTC ATCAGCTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAATTT TCGGATGCAA
Importance of organism and transcriptomes	Cyanoptyche gloeocystis (Pascher, 1929) is colonial a Glaucophyte and represent one of the only two species described in the order Gloeochaetales (Kies and Kremer, 1986). This particular isolate was collected in Portugal 1989. Despite the evolutionary relevance of Glaucophytes the genomic data available for this algal lineage is very limited. We aim to study genetic diversity within this early diverging algal group.
Additional citations and references	Kies, L. & Kremer, B.P. (1986). Typification of the Glaucocystophyceae. Taxon 35: 128-133
Primary citation for organism's characterization, if available	Pascher, A. (1929). Studien uber symbiosen. Uber einige Endosymbiosen von Blaualgen in Einzellern. Jahrb. Wiss. Bot. 71: 386-462.
Collection date	01-JAN-89
Sample collection site	OTHER
Other collection site info	Sintra, Monserrate Park
ENVO term for habitat - primary term	Aquatic: freshwater
Habitat	freshwater habitat
Country	PORTUGAL
Experimental Data
Date of experiment	27-OCT-11
Growth medium	SVM (Standard Volvox Media). Based on Kirk and Kirk (1983) Dev. Biol. 96:493?506
Modifications to growth medium	none
Temperature (ºC)	18
pH	8
Light (µmol photons / m2 / sec)	200
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	500
Phosphate (μmol/L)	230
Total Fe (nmol/L)	2000
Trace elements (total) (nmol/L)	20
Urea (μmol/L)	500
Prey organism, if applicable (genus and species)	NA
Investigation type	Eukaryotes