Phaeocystis antarctica Caron Lab Isolate

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Environmental Data
Principle Investigator(s)	David Caron
External sample ID	P.antarctica
NCGR Sample ID	MMETSP1100
Sample accession number	CAM_SMPL_002642
Assembly accession number	CAM_ASM_000460
Combined Assembly Name	N/A
Genus	Phaeocystis
Species	antarctica
Strain	Caron Lab Isolate
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	33657
18S rRNA	AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCGACTTGTACT GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTTCTTTGATGGTACCTTGCTACTTGGATACCCGTAGTAATTCTA GAGCTAATACATGCAGGACTGCCCGACTTCGGAAGGGCTGTATTTATTAGATAAGAAACCATCTCGGGTCCGCCCGGTTG TGTGCTGAGTCATAATAACTGCTCGAATCGCACGGCTCTACGCCGGCGATGGTTCATTCAAATTTCTGCCCTATCAGCTT TCGATGGTAGGATAGAGGCCTACCATGGCGTTAACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGA AATGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTGCCCGAATCCTGACACAGGGAGGTAGTGACAAGAAATAA CAATACAGGGCTACTTCTAGTCTTGTAATTGGAATGAGTACAACTTACATCTCTTCACGAGGATCAATTGGAGGGCAAGT CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCGGATT TCGGGTCGGGCCGAGCGGTCTGCCGATGGGTATGCACTGTTTGGCGCGGCCTTCTTTCCGGAGACCGCGGCTACTCTTAA CTGAGCGGGCGTGGGAGACGGATCGTTTACTTTGAAAAAATCAGAGTGTTTCAAGCAGGCAGCTCGCTCTTGCATGGATT AGCATGGGATAATTGAATAGGACTCTGGTGCTATTTTGTTGGTTTCGAACACCGGAGTAATGATTAACAGGGACAGTCAG GGGCACTCGTATTCCGTCGAGAGAGGTGAAATTCTCAGACCAATGGAAGACGAACCACTGCGAAAGCATTTGCCAGGGAT GTTTTCACTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTCTTAACCATAAACCATGCCGA CTAGGGATTGGAGGGTGTTCGTTTCGACCTCTTCAGCACCTTACGGGAAACTAAAGTCTTTGGGTTCCGGGGGGAGTATG GTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACG GGGAAACTTACCAGGTCCAGACATTGTTAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGATGGGTGGTGGTGCATGG CCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGTAGCCTGCTAAATAGTTCCGCGAA CTTCTTGTTGGCGGTCGACTTCTTAGAGGGACAACTTGTGACCAACAAGTGGAAGTTTGCGGCAATAACAGGTCTGTGAT GCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGAAGCACTCAACGAGTCCACCTCGACCGACAGGTCTGGGAAACC TTTTGAAATTGCTTCGTGATGGGGATAGATTATTGCAACTATTAATCTTCAACGAGGAATTCCTAGTAAGCGCATGTCAT CAGCGTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAGGCCCCC GGACTGGGGCGACGCATTCGGTTCGCCGGACGCGACGCTCCGGGAAGCTGTCCAAATCTTATTATTTAGAGGAAGGAGAA GTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC
Importance of organism and transcriptomes	This second set of 15 sample submissions includes mixotrophic protists and related heterotrophs from a wide diversity of taxa. We hope looking at these transcriptomes will shed light on evolutionary and ecological adaptation of single celled eukaryotes. While Phaeocystis is not known to be mixotrophic it is related to other mixotrophic organisms.
Additional citations and references	Caron, D.A. 2000. Symbiosis and mixotrophy among pelagic microorganisms. In: Kirchman, D.L. (Ed.), Microbial ecology of the oceans, John Wiley & Sons, Inc., New York, pp. 495-523.
Latitude	-77.625
Longitude	-179.23
Salinity (psu)	36
Temperature (ºC)	1
Collection date	03-DEC-05
Sample material (e.g. "seawater," "sediment," etc.)	seawater
Habitat	marine habitat
Country	ANTARCTICA
Experimental Data
Date of experiment	11-OCT-11
Growth medium	Marine Media K
Modifications to growth medium	Soil extract
Temperature (ºC)	1
Salinty (psu)	36
pH	8
Light (µmol photons / m2 / sec)	300
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Investigation type	Eukaryotes