Symbiodinium sp. CCMP421

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Environmental Data
Principle Investigator(s)	William Wilson
External sample ID	CCMP421 T0 Control
NCGR Sample ID	MMETSP1110
Sample accession number	CAM_SMPL_003116
Assembly accession number	CAM_ASM_000834
Combined Assembly Name	N/A
Genus	Symbiodinium
Species	sp.
Strain	CCMP421
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	174948
18S rRNA	TCAGTTTAAGTTTTTATACGGCGAAACTGCGAATGGCTCATTAAAGCAGTTATAGTTCATTTGATGGTCACTGCTACATGGATAACTGTGGTAATTCTAGAGCTAATACATGCGCAAAAACCCGACTTTGCAGGAGGGTTGTGTTTATTAGATACAGAACCAACCCAGGCTCTGCCTGGACATGTGGTGATTCATGGTAACTTGATGAATCGTGTGGCTCTGCCGACGATGCATCATTCAAGTTTCTGACCTATCAGCTTCCGACGGTAGGGTATTGGCCTACCGTGGCAATGACGGGTAACGGAGAATTAGGGTTTGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCTAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCATCCATGTCTTGTAATTGGAATGAATAGAATTTAAACCCCTTTGTGAGTATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTGGATTTCTGTTGGGGGTGACCGGTCCGCCTTCTGGGTGTGCATCTGGTTCAGCCTTGACATCTTCCTGAGGAACGTGTCTGCACTTCGCTGTGTGGCGCGGCATTTAGGACTTTTACCTTGAGGAAATTAGAGTGTTTCAAGCACGCGCACGCCTTGAATACATTAGCATGGAATAATAAGATAGGACCCCAGTTCTATTTTGTTGGTTTCTAGAGCTGAGGTAATGATCGATAGGGATAGTTGGGGGCATTCGTATTTAACTGTCAGAGGTGAAATTCTTGGATTTGTTAAAGACGGACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATCAAGAACGAAAGTAGGGGATCGAAGACGATCAGATACCGTCCTAGTCTTAACCATAAACTATGCCAACTAGAGATTGGAGGTCGTTAATTGCACGACTCCTTCAGCACCTTACGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACATGTAACCTCGGTTATATGGGCAACTTCTTAGAGGGACTTTGTGTGTCTAACGCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACACTGATGCGCTCAACGAGTTTTTGATCTTGCCTGAAATGGCTGGGTAATCTTTTTAAAATGCATCGTGATGGGGATAGATCATTGCAATTATTGATCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGTGCTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAGTGATCCGGTGAATAATTCGGACTGACGCAGTGCTCAGCTTCTGGACGTTGCGTTGGAAAGCTTCATGAACCTTATCACTAGGA
Importance of organism and transcriptomes	CCMP421 is a putatively free-living marine dinoflagellate belonging to the genus Symbiodinium. The genus Symbiodinium consists almost exclusively of symbiosis-forming species, thus the potentially free-living nature of this strain provides a scientifically interesting study organism. As part of our ongoing research into latent viruses in Symbiodinium, we would like to sequence transcriptomes from plus and minus UV treated cultures of this strain (preliminary flow cytometry evidence from our lab suggests this strain harbors a latent virus infection which can be induced by exposure to UV radiation).
Additional citations and references
Latitude	-41.27
Longitude	174.795
Collection date	01-MAY-80
Sample collection site	Pacific_Ocean
Habitat	marine habitat
Country	NEW ZEALAND
Experimental Data
Date of experiment	01-OCT-11
Growth medium	f/2 amended seawater
Modifications to growth medium	f/2 for all nutrients, no Na2SiO3 added
Temperature (ºC)	25
Salinty (psu)	34
pH	8.1
Light (µmol photons / m2 / sec)	200
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Trace elements (total) (nmol/L)	12794
Investigation type	Eukaryotes
Other experimental metadata available	RNA will be harvested from 1 L of culture during normal exponential growth