Aplanochytrium stocchinoi GSBS06

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Environmental Data
Principle Investigator(s)	Jackie Collier
External sample ID	Aplano. GSBS06 horse serum
NCGR Sample ID	MMETSP1347
Sample accession number	CAM_SMPL_002872
Assembly accession number	CAM_ASM_000690
Combined Assembly Name	Aplanochytrium-stocchinoi-GSBS06
Genus	Aplanochytrium
Species	stocchinoi
Strain	GSBS06
Clonal	Yes
Axenic	Yes
Prelim. NCBI Taxon ID	215587
18S rRNA	GTCATACGCTCGTcTCAAAGATTAAGCCATGCATGTCTAaGTGCAAGCACTTGTACTGTGAAACTGCGAATGGCTCATTATATCAGTTATAGTTTATTTGATAGATGCCTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCAACAAAGCCCAACTTTTCGGACGGGCTGTATTTATTAGATAGAAACCAATGCAGGGTTTTCCCTGGTGTTGTGGTGAGTCATAATAACTAaGCGAATCGCAGTGGCTTCGGCCGGCGATGAATCATTCAAGTTTCTGCCCTATCAGCTGTCGATGGTAGGGTATTGGCCTACCATGGCGTTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTACCCAATCCTGATACGGGGAGGTAGTGACAATAAATAACAATACGGGGCCCATTGGGTCTTGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCTGGTAGGAGCGACCACTCCGGACTTGATTGTTCGTGTATTGTGTTGTCTCCAGCCATCCTTGTGGAGAACTTTTCTTGCATTAATTTGTTGGGATTGGGACCCGCATCGTTTACTGTGAAAAAATTAGAGTGTTTAAAGCAGGCAATCGCTTGAATACATTAGCATGGAATAATAAGATAGGACTTTGGTACTATTTTGTTGGTTTGCATACCAAATTAATGATCAACAGGAACAGTTTGAGGATATTCGTATGAACATGTCAGAGGTGAAATTCTTGGATTTTGATCAGACGAACTACTGCGAAAGCATTTATCAAGGATGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATTAGATACCATCGTAGTCTTAACCATAAACTATGCCGACTAGGGATTGGCGGACGTTGTCTATATGACTCCGCCAGCACCTCATGAGAAATCAAAGTCTTTGGGTtCcGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAATAGTGTGCATATTCGAAAGAATGTGTTCGACTTCTTAGAGGGACATTTCGGTTTTACCGGAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACAATGATGAATTCAACGAGTTTATAACCTTGGTTGAAAAGCCTGGGTAATCTTTTGAACTTTCGTCGTGATGGGGCTAGACCCTTGCAATTATTGGTCTCCAACGAGGAATTCCTAGTAAACGCAAGTCATCAGCTTGCATTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAATGGTCCGGTGAAATCTTCGGATCGAGACTTTGATTTGTTTACTCAAAGCGCTGTTTCGAGAAGTTGATTAAACCTTACCATTAGAGGAAGggaagt
Importance of organism and transcriptomes	The labyrinthulomycetes (including labyrinthulids, oblongichytrids, thraustochytrids, and aplanochytrids) are ubiquitous, diverse, and abundant marine protists (e.g. Collado Mercado et al. 2010 and references therein). They are thought to live mainly as saprobes, obtaining their nutrition from non-living particulate organic matter (POM) of algal, higher plant, or animal origin. Thus, while labyrinthulomycetes are not fungi in a taxonomic sense, they function as fungi in an ecological sense, likely playing important roles in the decomposition of marine POM. This isolate, Aplanochtyrium GSBS06, was isolated from sediment collected in Great South Bay (Long Island, NY, USA) in July 2008. The 18S rDNA sequence of this strain is ~99% identical to Aplanochytrium PBS07, but the two strains also show different growth forms and media preferences. Comparative analysis of the genes expressed by this organism during growth on a variety of substrates, and comparison of GSBS06 with PBS07, will provide unprecedented insight into the physiology and role of aplanochytrids in marine ecosystems.
Additional citations and references	Collado Mercado, E., Radway, J. C. & Collier, J. L. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58:215-28. Damare, V. & Raghukumar, S. 2010. Association of the stramenopilan protists, the aplanochytrids, with zooplankton of the equatorial Indian Ocean. Mar. Ecol. Prog. Ser. 399:53-68. Yokoyama, R. & Honda, D. 2007. Taxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomic characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov. Mycoscience 48:199-211. Leander CA, Porter D (2000) Redefining the genus Aplanochytrium (Phylum Labyrinthulomycota). Mycotaxon 76:439-444. Leander CA, Porter D (2001) The Labyrinthulomycota is comprised of three distinct lineages. Mycologia 93:459-464.
Primary citation for organism's characterization, if available	Moro I, Negrisolo E, Callegaro A, Andreoli C. 2003. Aplanochytrium stocchinoi: a new Labyrinthulomycota from the southern ocean (Ross Sea, Antarctica). Protist 154(3-4):331-40.
Latitude	40.7
Longitude	-73.1
Depth (m)	1
Salinity (psu)	25
Collection date	10-JUL-08
Sample collection site	OTHER
Other collection site info	Great South Bay, Long Island, NY
Sample material (e.g. "seawater," "sediment," etc.)	Sediment
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Other
Habitat description	Sediment from Great South Bay, Long Island, NY, USA
Other environmental metadata available	none available
Other environmental metadata available	none available
Habitat	marine habitat
Country	UNITED STATES
Experimental Data
Date of experiment	24-MAY-12
Growth medium	artificial seawater with 1% horse serum (0.8% agar plates)
Modifications to growth medium	artificial seawater (25 grams per liter Instant Ocean) plus 0.8% agar autoclaved, then filter sterilized horse serum (1% vol:vol) added just before pouring plates
Temperature (ºC)	23
Salinty (psu)	25
pH	8
Pressure (atm)	1
Investigation type	Eukaryotes
Other experimental metadata available	Harvested 502 mg (wet weight) biomass from 50 plates after 16 days of growth