Home :: List of Publicly Available Datasets :: Aplanochytrium stocchinoi GSBS06

Aplanochytrium stocchinoi GSBS06

Downloads:


Principle Investigator(s) Jackie Collier
External sample IDAplano. GSBS06 Artemia detritus
NCGR Sample IDMMETSP1348
Sample accession numberCAM_SMPL_002882
Assembly accession numberCAM_ASM_000700
Combined Assembly NameAplanochytrium-stocchinoi-GSBS06
GenusAplanochytrium
Speciesstocchinoi
StrainGSBS06
ClonalYes
AxenicYes
Prelim. NCBI Taxon ID215587
18S rRNA
Importance of organism and transcriptomesThe labyrinthulomycetes (including labyrinthulids, oblongichytrids, thraustochytrids, and aplanochytrids) are ubiquitous, diverse, and abundant marine protists (e.g. Collado Mercado et al. 2010 and references therein). They are thought to live mainly as saprobes, obtaining their nutrition from non-living particulate organic matter (POM) of algal, higher plant, or animal origin. Thus, while labyrinthulomycetes are not fungi in a taxonomic sense, they function as fungi in an ecological sense, likely playing important roles in the decomposition of marine POM. This isolate, Aplanochtyrium GSBS06, was isolated from sediment collected in Great South Bay (Long Island, NY, USA) in July 2008. The 18S rDNA sequence of this strain is ~99% identical to Aplanochytrium PBS07, but the two strains also show different growth forms and media preferences. Comparative analysis of the genes expressed by this organism during growth on a variety of substrates, and comparison of GSBS06 with PBS07, will provide unprecedented insight into the physiology and role of aplanochytrids in marine ecosystems.
Additional citations and referencesCollado Mercado, E., Radway, J. C. & Collier, J. L. 2010. Novel uncultivated labyrinthulomycetes revealed by 18S rDNA sequences from seawater and sediment samples. Aquatic Microbial Ecology 58:215-28. Damare, V. & Raghukumar, S. 2010. Association of the stramenopilan protists, the aplanochytrids, with zooplankton of the equatorial Indian Ocean. Mar. Ecol. Prog. Ser. 399:53-68. Yokoyama, R. & Honda, D. 2007. Taxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomic characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov. Mycoscience 48:199-211. Leander CA, Porter D (2000) Redefining the genus Aplanochytrium (Phylum Labyrinthulomycota). Mycotaxon 76:439-444. Leander CA, Porter D (2001) The Labyrinthulomycota is comprised of three distinct lineages. Mycologia 93:459-464.
Environmental Data
Primary citation for organism's characterization, if availableMoro I, Negrisolo E, Callegaro A, Andreoli C. 2003. Aplanochytrium stocchinoi: a new Labyrinthulomycota from the southern ocean (Ross Sea, Antarctica). Protist 154(3-4):331-40.
Latitude40.7
Longitude-73.1
Depth (m)1
Salinity (psu)25
Collection date10-JUL-08
Sample collection siteOTHER
Other collection site infoGreat South Bay, Long Island, NY
Sample material (e.g. "seawater," "sediment," etc.)Sediment
ENVO term for habitat - primary termAcquatic: marine
ENVO term for habitat - secondary termOther
Habitat descriptionSediment from Great South Bay, Long Island, NY, USA
Other environmental metadata availablenone available
Other environmental metadata availablenone available
Habitatmarine habitat
CountryUNITED STATES
Experimental Data
Date of experiment11-JUN-12
Growth mediumartificial seawater with 0.125% Artemia detritus (0.8% agar plates)
Modifications to growth mediumartificial seawater (25 grams per liter Instant Ocean), 0.125% freeze dried and ground Artemia (wt:vol), solidified with 0.8% agar
Temperature (ÂșC)23
Salinty (psu)25
pH8
Pressure (atm)1
Investigation typeEukaryotes
Other experimental metadata availableHarvested 652 mg (wet weight) biomass from 10 plates after 11 days of growth