Hemiselmis tepida CCMP443

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	Hte443.1
NCGR Sample ID	MMETSP1355
Sample accession number	CAM_SMPL_002903
Assembly accession number	CAM_ASM_000721
Combined Assembly Name	N/A
Genus	Hemiselmis
Species	tepida
Strain	CCMP443
Clonal	Unknown
Axenic	Unknown
Prelim. NCBI Taxon ID	464990
18S rRNA	AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTTAGTGTAAATAACTCTTATGAAACTGCG AATGGCTCATTAAATCAGTTATCGTTTATTTGATGGTCTCTTACTACATGGATAACCGTAGTAATTCTAG AGCTAATACATGCACAAAGGCTCGACTCACGAAGAGCTGTATTTATTAGATTCAAAGCCGACCTTGTTTT GGTGATTCATAATAACTTTTCGAACCTCATGCCCTTGCGGTGGAGGTGATTCATTCAAATTTCTGCCCTA TCAACTTTCGATGGTAGGATAGAGGCCTACCATGGTTTTAACGGGTGACGGAGAATTAGGGTTCGATTCC GGAGAGGGAGCCTGAGAGACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGA CTCGGGGAGGTAGTGACAATAAATAACAATACAGGGCTTACAGTCTTGTTATTGGAATGAGAACAATTTA AACCCCTTAACGAGGATCAATTAGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTAATAG CGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCGGATGTCGGGCTCGGGCAGGCTGTCGGCCTCT GGTCGGACGGCAGGCTCGGGTCTTTTTGCCTGGGGGCTCCGTCACACTTAACTGTGGGGCGGTAGGTCGC AGGCCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTACGCTTGAATACATTAGCATGGAATAA TGGAATAGGACTTTGGTGCTATTTTGTTGGTTTATGGGACTGAAGTAATGATTAATAGGGACAGTTGGGG CCGTTTATATTTCGTTGTCAGAGGTGAAATTCTTGGATTTACGAAAGATAAACTTCTGCGAAAGCATTCG GCAAGGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTCTT AACCATAAACTATGCCGACTAGGGATCAGTGGATGTCAATTTGCGACTCCATTGGCACCTTGTGAGAAAT CAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACC ACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGG ATTGACAGATTGAAAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTG ATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGTTACGCGAATATTTATCGTG GCGATACTTCTTAGAGGGACTATTTGTGTTTAATGAATGGAAGTTTGAGGCAATAACAGGTCTGTGATGC CCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGAACGCAACGAGCTCTACCCTGCTCCGAAAGGT CCGGGTAAACTTGTGAAAGTTCATCGTGATGGGGATAGATTATTGCAATTATTAATCTTCAACGAGGAAT TCCTAGTAAGCGTGATTCATCAGATCGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTC CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGTCTTGGCTTGGCAACTTGCTTTGACTGCGA AAAGTTGATTAAACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGG AAGGATCATTG
Importance of organism and transcriptomes	Hemiselmis tepida is a cryptophyte alga. Cryptophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between cryptophytes and other red algal secondary plastid-bearing organisms will also be possible.
Additional citations and references
Experimental Data
Date of experiment	01-JUL-12
Growth medium	K
Modifications to growth medium	K without silicate
Temperature (ºC)	23
Salinty (psu)	35
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Ammonium (μmol/L)	50
Phosphate (μmol/L)	10
Trace elements (total) (nmol/L)	122766