Hemiselmis rufescens PCC563

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	Hru563.1
NCGR Sample ID	MMETSP1357
Sample accession number	CAM_SMPL_002914
Assembly accession number	CAM_ASM_000732
Combined Assembly Name	N/A
Genus	Hemiselmis
Species	rufescens
Strain	PCC563
Clonal	Unknown
Axenic	Unknown
Prelim. NCBI Taxon ID	77926
18S rRNA	CAGTAGTCATATGCTTGTCTTAAGGATTAAGCCATGCATGTCTCAGTATAAACGATTTTATACGGTGAAA CTGCGAATGGCTCATTACAACAGTTATAGTTTATTTGATCGTGCGGTACTACGTGGATAACCGTAGGAAT TCTAGAGCTAATACATGCCCCATCGCTTTCGAAATTTTTCTCCTTTAAGGAAAAAAATTAGAAAGTGGGA TTTATTAGATCAAAAACCAATCGCTTTAGAGCGTGGCGGGATGAATCATAATAACTTTCCGAATCGCAAG GCTATAAATTGAGCCGGCGATATATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGGTAGTGGC CTACCATGGTGTTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC CACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACTAAGGGAGGTAGCGACAAGAAATAA CGATAAAAGGCCTTTTCAGGTCTGTTTATTGGAATGAGGATAATTTACACCCCTTCTCGAGTATCCATTG GAGGGCAAGCCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGTGTATATTAAAGTTGTTGCAGTT AAAAAGCTCGTAGTCGAATGTAGAGTCTTGAGCGCCGAGATGTCTTTTTTTATTAGGAGACAATTTTTTT TGGCGTTCTAGATTCTTTTTTGCCGTTTACTATTTGTTTTTCGTTAAACGATTTTGGTACATCGGTAATT TTTACTGTGAATAAATTAGAGTGTTCATGGCAGGCCAAGGGCTTAAATATCTCAGCATGGAATAATAGAA TAGGACTTCTGGTTCTATTTTGTTGGTTTTTGGAGCCGAAGTAATGATTAATAGGAATAGTTGGGGGCAC TCGTACTCCGTTGTCAGAGGTGAAATTCTTGGATTTACGGAAGACGAACAACTGCGAAAGCATCTGCCAA GGATGTTTTCATTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATTAGATACCGTCGTAGTCTTAACC ATAAACTATGCCGACTGGGGATTGGTAGATGTATCAATAACGACTCTATCAGCACCCTATGAGAAATCAA AGTCTCTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACC AGGCGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAAACTTACCAGGTCCGGACATAGGAAGGATT GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT TGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACTTACTAATTAGTTACCTGGGCGGCAACGTCCAAG GAAAAAAATTTCTTAGTAGGACGATCCGTGATTAGCGGATGGAGGTGTGAGGCAATAACAGGTCTGTGAT GCCCTTAGATGTTCTGGGCCGCACGCGCGCTACAATGATGCAATCAACGAGTGCTGTACCTTAACCGAAA GGTTTGGGAAAACTTGCAAATTGCATCGTGTTAGGGATAGATGATTGCAATTATTCATCTTGAACGAGGA ATGCCTTGTAAGCGTGAGTCATCAACTCGCGCTGAATACGTCCCTGCCCTTTGTACA
Importance of organism and transcriptomes	Hemiselmis rufescens is a cryptophyte alga. Cryptophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between cryptophytes and other red algal secondary plastid-bearing organisms will also be possible.
Additional citations and references
Experimental Data
Date of experiment	01-JUL-12
Growth medium	f/2-Si
Temperature (ºC)	20
Salinty (psu)	35
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	26
Trace elements (total) (nmol/L)	115345