Bigelowiella natans CCMP1242

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	Bna1242.1
NCGR Sample ID	MMETSP1358
Sample accession number	CAM_SMPL_002915
Assembly accession number	CAM_ASM_000733
Combined Assembly Name	N/A
Genus	Bigelowiella
Species	natans
Strain	CCMP1242
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	227086
18S rRNA	GTCATATGCTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTATAAACAACTTTGTACTGTGAAACTGCA GATGGCTCATTAAATCAGAAATAATTTCTTTGATGGTGAAAATCTACACGGATACCCGTAGTAATTCTAG AGCTAATACGTGCGTAAACTCCATTGGAGGTATTTATTAGATCCGAACCAGCCCCTTCGGGGTTTTCTGG TGAATCATGATAACTGTTCGAATCGCCAGGCTCTGCCAGGCGATAGTTCATTCAAATTTCTGCCCTATCA GCTATTGACGGTAGTGTAGTGGACTACCGTGGCCGTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGA GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGACAC GGGGAGGTAGTGACAAGAAATATCGTGACTGTGCCCTTTGTGGCAGAGTCTTCGAAATGAGCAATACGTA AATGCCTTTGCGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG CGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATATCTGGCTTTGCACGGACGGCCCGCCTT CGGGTTGAGTGCTGGAAGTGCTAGCCTATTTTCTGGTTGGCGTTCTTTCCCTTAATTGTGGCGAGTTTCG CGATCCGGATCGTTTACTTTGAAGAAATTCGAGTGTTCAAAGCAGGCTTACGCCGTGAAGATTGCAGCAT GGAATAATGACATAGGACTATCGTGTTCTATTTTGTTGGTTCTGGGACCGTAGTAATGATTGATAGGGAC AGTTGGGGGTGCTGCTATTCCGCGGCCAGAGGTGAAATTCTCAGATTCCCGGAAGAGCAACTTATGCGAA AGCATTCACCAAGGATGTCTTCTTTAATCAAGAACGAAAGTAGGGGGATCGAAGACGATCAGATACCGTC GTAGTCTCTACCATAAACTATGCCGACTAGGGATTGGTGGACGTTGTTTTATCCGACTCCATCAGCACCT TATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACG GAAGGACACCACCAGAAGTGGAACTTGCGGCTTAATTTGACTCAACACGGGAAAACTTTCCAGGTCCAGA CATAGTAAGGATTGACAGATTGAAGATCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTT GGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCGACCTGCTAAATAGGTTCAGTATCG ATGCGTCGATGCTTGGAGCTTCTTAGAGGGACTATCGGATCCAAACCGGTGGAAGTGCGAGGCAATAACA GGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGTGTTACACTGACGCATTCATCAAGCGCCACACC TTGGCTCGAGAGGCCTCGGTAATCTTGAAAGTGCGTCGTGTTAGGGATAGATTATTGTAATTATTAGTCT TGAACGAGGAATTTCTAGTAAACGCGAGTCATCAACTCGCATTGATTACGTCCCTGTCCTTTGTACACAC CGCCCGTCGCTACTACCGATTGAATCATTTGGTGAGGCTCACGGACTGTCGTGCTTCCTTCCTCGTGTTG GTTGTACTTCTTGGGAAGTGATACAAACCTG
Importance of organism and transcriptomes	CCMP strain 1242 is a chlorarachniophyte alga closely related to Bigelowiella natans. Chlorarachniophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between chlorarachniophytes and other red and green algal secondary plastid-bearing organisms will also be possible.
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Experimental Data
Date of experiment	01-JUL-12
Growth medium	K
Modifications to growth medium	K without silicate
Temperature (ºC)	23
Salinty (psu)	35
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Ammonium (μmol/L)	50
Phosphate (μmol/L)	10
Trace elements (total) (nmol/L)	122766