Bigelowiella longifila CCMP242

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Environmental Data
Principle Investigator(s)	John Archibald
External sample ID	Blo242.1
NCGR Sample ID	MMETSP1359
Sample accession number	CAM_SMPL_002927
Assembly accession number	CAM_ASM_000745
Combined Assembly Name	N/A
Genus	Bigelowiella
Species	longifila
Strain	CCMP242
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	552665
18S rRNA	ATGGATCCGTAGTCATATGCTTGTCTCAAAGACTAAGCCATGCATGTCTAAGTATAAACAACTTTGTACT GTGAAACTGCAGATGGCTCATTAAATCAGAAATAATTTCTTTGATGGTGAAAATCTACACGGATACCCGT AGTAATTCTAGAGCTAATACGTGCGTAAACTCCATTGTGAGGTATTTATTAGATCCGAACCAGCCCCTTC GGGGTATTTTGGTGAATCATGATAACTGTTCGAATCGCCAGGCTCTGCCAGGCGATAGTTCATTCAAATT TCTGCCCTATCAGCTATTGACGGTAGTGTAGTGGACTACCGTGACCGTAACGGGTGACGGAGAATTAGGG TTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCCCAAATTACC CAATCCCGACACGGGGAGGTAGTGACAAGAAATATCGTGACTGTGCCCTTTGTGGCAGAGTCTTCGAAAT GAGCAATACGTAAATGCCTTTTCGAGGGACATTGGAGGGACAAAGATCTGGTTACCAGGCAAGCCGCGGT AATTCCAGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATATCTGGCCTTT GCACGGACGGCCCGCCTTCGGGTTGAGTGCTGGAAGTGCAGGTCTATATTCTGGTTGGCGTGCTTTCCCT TTATTGTGGCGAGTATCGCGATCCGGATCGTTTACTTTGAAGAAATTCGAGTGTTCAAAGCAGGCTTACG CCGTGAAGATTGCAGCATGGAATAATGACATAGGACTATCGTGTTCTATTTTGTTGGTTCTGGGACCGTA GTAATGATTGATAGGGACAAGTTGGGGGTGCTGCTATTCCGCGGCCAGAGGTGAAATTCTCAGATTCCCG GAAGAGCAACTTATGCGAAAGCATTCACCAAGGATGTCTTCTTTAATCAAGAACGAAAGTAGGGGGATCG AAGACGATCAGATACCGTCGTAGTCTCTACCATAAGCTATGCCGACTAGGGATTGGTGGACGTTGTTTTA TCCGACTCCATCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTG AAACTTAAAGGAATTGACGGAAGGACACCACCAGAAGTGGAACTTGCGGCTTAATTTGACTCAACACGGG AAAACTTTCCAGGTCCAGACATAGTAAGGATTGACAGATTGAAGATCTTTCTTGATTCTATGGGTGGTGG TGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCGACCTG CTAAATAGGTTCAGTATCGATGCGTCGATGCTTGGAGCTTCTTAGAGGGACTATCGGATCCAAACCGGTG GAAGTGCGAGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGCCGCACGCGTGTTACACTGACGCAT TCATCAAGCGCCACACCTTGGCTCGAGAGGCCTCGGTAATCTTGAAAGTGCGTCGTGTTAGGGATAGATT ATTGTAATTATTAGTCTTGAACGAGGAATTTCTAGTAAACGCGAGTCATCCACTCGCATTGATNACGTCC CTGTCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATCATTTGGTGAGGCTCACGGACTGTCGTG CATCCTTCCTCGTGTTGGTTGTGCTTCTTGGGAAGTGATACAAACCTGATGATTTAGAGGAAGTAAAAGT CGTAACAAGGTTTCCGTAGGTGAACCTGCGGATCCAT
Importance of organism and transcriptomes	Bigelowiella longifila is a chlorarachniophyte alga. Chlorarachniophytes are unusual among secondary plastid-containing algae in that they still possess the nucleus (nucleomorph) and cytoplasm of their algal endosymbionts in a highly reduced and simplified form. Investigating the nuclear genome of these organisms will improve our understanding of secondary endosymbiosis and the integration of their respective hosts and endosymbionts at the genetic, biochemical, and cellular levels. A better understanding of the relationships between chlorarachniophytes and other red and green algal secondary plastid-bearing organisms will also be possible.
Additional citations and references
Experimental Data
Date of experiment	01-JUL-12
Growth medium	blue star agar
Modifications to growth medium	blue star agar without silica
Temperature (ºC)	20
Salinty (psu)	35
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	882
Phosphate (μmol/L)	26
Trace elements (total) (nmol/L)	115345