Marine Microbial Eukaryote Transcriptome Sequencing Project
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Euplotes crassus CT5
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| Principle Investigator(s) | Cristina Miceli |
|---|---|
| External sample ID | ECTR |
| NCGR Sample ID | MMETSP1380 |
| Sample accession number | CAM_SMPL_003159 |
| Assembly accession number | CAM_ASM_000877 |
| Combined Assembly Name | N/A |
| Genus | Euplotes |
| Species | crassus |
| Strain | CT5 |
| Clonal | Unknown |
| Axenic | No |
| Prelim. NCBI Taxon ID | 5936 |
| 18S rRNA | |
| Importance of organism and transcriptomes | The E. crassus transcriptome will provide a good reference of a mesophilic Euplotes to be compared with data of the psychrophilic E. focardii MMETSP0205 |
| Additional citations and references | Turanov AA, Lobanov AV, Fomenko DE, Morrison HG, Sogin ML, Klobutcher LA, Hatfield DL, Gladyshev VN. Science. 2009 Jan 9;323(5911):259-61 |
| Environmental Data | |
| Sample collection site | Tyrrhenian Sea |
| Sample material (e.g. "seawater," "sediment," etc.) | seawater and sediment trap material |
| ENVO term for habitat - primary term | Acquatic: marine |
| ENVO term for habitat - secondary term | Sediment |
| Habitat | marine habitat |
| Country | ITALY |
| Experimental Data | |
| Date of experiment | 28-JAN-13 |
| Growth medium | Cells were grown in natural sea water collected in Adriatic Sea. Twice per week 50ml of the algae Dunaliella tertiolecta grown in Walne's medium (http://www.ccap.ac.uk/media/recipes/Walnes.htm) were added to each one-liter cell cultures |
| Temperature (ºC) | 25 |
| Salinty (psu) | 38 |
| pH | 8 |
| Day portion of day:night cycle in hours | 12 |
| Night portion of day:night cycle in hours | 12 |
| Nitrate (μmol/L) | 300 |
| Ammonium (μmol/L) | 100 |
| Phosphate (μmol/L) | 50 |
| Silicate (μmol/L) | 100 |
| Trace elements (total) (nmol/L) | 750 |