Ammonia sp. Unknown

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Environmental Data
Principle Investigator(s)	Jan Pawlowski
External sample ID	12AB
NCGR Sample ID	MMETSP1384
Sample accession number	CAM_SMPL_003162
Assembly accession number	CAM_ASM_000880
Combined Assembly Name	N/A
Genus	Ammonia
Species	sp.
Strain	Unknown
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	29189
18S rRNA	AAGGGCACCACAAGAACGCGTGGAGNNNNGGCTTAATTTGACTCAACGCGGGAAATCTTACCGGGTCCGG ACACACTGAGGATTGACAGATATACGCGCATATACTCTCTCGTGGAGTATATACGTTCAAAGATGCTAGT TCTTTCATGATTATGTGATAGGTGGTGCATGGCCGTTCTTAGCTCGTGGAGTGATCTGTCTGCTTAATTG CGTATCAATAATAGAGACCTAGTATACGCGTAGACTCGTATGGTAGTGACCCCCTCTTAATTGAGGCGCG TGTCGCACATACGTTATACGCACTGGTCTCAGATAGCAACGAACGTGACCGTACTCTATTGTTGCAGTGA AAATGTTGCTTCGGCAACAAACCCACTGCTTAGTACGCGTGTATTTCGGTACGCGCCGTACATTAAACTA TAGAGACCCGCTGTTTCTTCTTTAAACCAGAGGAAGGATACGGCAATAACAGGTCTGTGATGCCCTCAGA TGTTCCGGGCTGCACACGTGCTACAATGATCATTGCACCGTGCATCTAACCCAATGTGCGCGGACGCCGC GTATACGTGTTTTCCTTTCGGGACTCATGTATATTGCTGAGCGACCGCGCCGAACCTACTTCGAAAGTAA AATTTCTCTGTGGGTAATCCATTAGAAGTAATGACTCGCATAGACCATGGCACACTATAAAAGTACGCGC AGGTTCTACCCGGCTCGCCTTTGTGTGAGTGCAGTGCGTAGCTTGTTGTTTCGTACGTGCCACCTCGTAT TAATTCGTACGTGGGGACAGATCATTGTTTAATTGTTGGTCTCGGTCTTAACTAGGAATGCCTTGTACGG GTCTCTGGTTCAACATACCACCCGGAATACNTCCCTGCCCTTTGTACACACCGCCCGTCGCTCTTACCGA TGGATTATACTATGAATCTATAGGACTGCCAAAGCTCTCACGCTCTCGCGCGGAAGCTTAGTGGAAATAT ATATGAATAGTGTGATCTAAAGGAAAGAGAAGTCGAACAAGGCA
Importance of organism and transcriptomes	Foraminifera are among the most abundant and diverse marine protists, characterized by granuloreticulopodia. Their tests or shells are an important component of marine sediments and widely used as biostratigraphic markers. Ammonia sp. is classified with the order Rotaliida for which no published EST exists up to date. The genus is common in the coastal waters and our strain was collected from the North Sea. To date, the molecular data on this organism, and foraminifers in general, is extremely poor represented in the available databases. Our knowledge on the gene expression repertoire and metabolic pathways used by foraminifers is still very limited. Additionally, transcriptomic data has shown to be a powerful tool for finding orthologous genes for further phylogenetic analysis, in an aim to resolve the phylogenetic relationships among the group and within the Eukaryotes.
Additional citations and references	i. Hayward, B., W., Holzmann, M., Grenfell, H., R., Pawlowski, J., 2004, Mar. Micropal., 50, 237-271
Primary citation for organism's characterization, if available	Linnaeus, C. (1758). Systema Naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Editio decima, reformata. Laurentius Salvius: Holmiae. ii, 824 pp.
Sample collection site	North Sea
ENVO term for habitat - primary term	Acquatic: marine
Habitat	marine habitat
Country	GERMANY
Experimental Data
Date of experiment	03-DEC-12
Growth medium	nonculturable
Temperature (ºC)	20
Salinty (psu)	30
pH	8
Day portion of day:night cycle in hours	10
Night portion of day:night cycle in hours	14
Nitrate (μmol/L)	12
Ammonium (μmol/L)	4
Phosphate (μmol/L)	2
Silicate (μmol/L)	1
Trace elements (total) (nmol/L)	15
Prey organism, if applicable (genus and species)	Bacteria and algae
Investigation type	Eukaryotes